A5044946533- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Pluripotent Stem Cells Research
- Trace Elements in Health
- TGF-β signaling in diseases
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- Neurobiology and Insect Physiology Research
- Cellular Mechanics and Interactions
- Zebrafish Biomedical Research Applications
- Renal and related cancers
- Antioxidant Activity and Oxidative Stress
- RNA Research and Splicing
- Retinoids in leukemia and cellular processes
- Heavy Metal Exposure and Toxicity
- Cell Adhesion Molecules Research
- CRISPR and Genetic Engineering
- Neuroscience and Neuropharmacology Research
- Biochemical Analysis and Sensing Techniques
- Gene expression and cancer classification
- Cancer-related gene regulation
- Behavioral and Psychological Studies
- Animal Nutrition and Physiology
The Francis Crick Institute
2016-2025
University College London
2025
National Park Service
2025
University of Florida
2024
North Florida/South Georgia Veterans Health System
2024
Geriatric Research Education and Clinical Center
2024
University of Pittsburgh
2024
University of Arizona
2024
Medical University of South Carolina
2006-2023
UConn Health
2022-2023
Gastrulation in the amphibian embryo is driven by cells of mesoderm. One genes that confers mesodermal identity Xenopus Brachyury (Xbra), which required for normal gastrulation movements and ultimately posterior mesoderm notochord differentiation development all vertebrates. Xbra a transcription activator, interference with activation leads to an inhibition morphogenetic during gastrulation. To understand this process, we have screened downstream target (Tada, M., Casey, E., Fairclough, L....
Abstract The mesoderm of amphibian embryos such as Xenopus laevis arises through an inductive interaction in which cells the vegetal hemisphere embryo act on overlying equatorial and animal pole cells. Three classes ‘mesoderm-inducing factor’ (MIF) that might be responsible for this vivo have been discovered. These are members transforming growth factor type β (TGF-β), flbroblast (FGF) Wnt families. Among most potent MIFs activins, TGF-βfamily, but RNA activin A B is not detectable until...
Thirty-four carotenoids, including 13 geometrical isomers and eight metabolites, in breast milk serum of three lactating mothers have been separated, identified, quantified, compared by high-performance liquid chromatography (HPLC)−photodiode array (PDA) detection−mass spectrometry (MS). Among the metabolites were two oxidation products lycopene four lutein/zeaxanthin. In addition, lutein, formed as a result dehydration this dihydroxycarotenoid under acidic conditions similar to those...
Proteins in the TGF-beta superfamily transduce their effects through binding to type I and II serine/threonine kinase receptors. Osteogenic protein-1 (OP-1, also known as bone morphogenetic protein-7 or BMP-7), a member of which belongs BMP subfamily, was found bind activin receptor (ActR-I), receptors IA (BMPR-IA) IB (BMPR-IB) presence (ActR-II) IIB (ActR-IIB). The affinity OP-1 ActR-II two- threefold lower than that A. A transcriptional activation signal transduced after complex ActR-I...
Activation of transforming growth factor β receptors causes the phosphorylation and nuclear translocation Smad proteins, which then participate in regulation expression target genes. We describe a novel Smad-interacting protein, SIP1, was identified using yeast two-hybrid system. Although SIP1 interacts with MH2 domain receptor-regulated Smads andin vitro, its interaction full-length mammalian cells requires receptor-mediated activation. is new member δEF1/Zfh-1 family two-handed zinc...
ABSTRACT Mouse embryos homozygous for a null mutation in nodal arrest development at early gastrulation and contain little or no embryonic mesoderm. Here, two Xenopus nodalrelated genes (Xnr-1 Xnr-2) are identified shown to be expressed transiently during embryogenesis, first within the vegetal region of late blastulae later marginal zone gastrulation, with enrichment dorsal lip. Xnrs mouse function as dosedependent dorsoanterior ventral mesoderm inducers whole explanted animal caps. Using...
Using a 1.5-tesla GE Signa MR scanner, we imaged the brains of 14 right-handed Tourette9s syndrome (TS) patients (11 men, three women), aged 18 to 49 years, who had minimal lifetime neuroleptic exposure. We also studied an equal number normal controls individually matched for age, sex, and handedness group-matched socioeconomic status. circumscribed basal ganglia on sequential axial images from spin-echo proton density-weighted acquisitions (TR 1,700, TE 20; slice thickness, 3 mm with 1.5-mm...
BMP is thought to induce hESC differentiation toward multiple lineages including mesoderm and trophoblast. The BMP-induced trophoblast phenotype a long-standing paradox in stem cell biology. Here we readdressed function hESCs mouse epiblast-derived cells. We found that BMP4 cooperates with FGF2 (via ERK) inhibit endoderm differentiation. These conditions induced cells high levels of BRACHYURY (BRA) coexpressed CDX2. BRA was necessary for preceded CDX2 expression; both genes were essential...
Regulating the MBT It has been known for more than 30 years that a defined number of cell divisions in frog embryo precede crucial developmental event called midblastula transition (MBT). Collart et al. (p. 893 , published online 1 August) now elucidate mechanism involved control MBT. DNA replication initiation factors are titrated out during early divisions, which controls elongation cycle and onset zygotic transcription
Numerous epidemiological studies have demonstrated that consuming large quantities of fruits and vegetables reduces the risk for several types human cancers. Carotenoids are abundant in been extensively studied as cancer preventive agents. A proposed mechanism action protective effect carotenoids against is based on their antioxidant capability. Recently, we isolated characterized 14 new carotenoids, including seven metabolites from extracts serum/plasma. This brings total number identified...
We have isolated a novel secreted molecule, Wise, by functional screen for activities that alter the anteroposterior character of neuralised Xenopus animal caps. Wise encodes protein capable inducing posterior neural markers at distance. Phenotypes arising from ectopic expression or depletion resemble those obtained when Wnt signalling is altered. In cap assays, can be induced family members, and induction these requires components canonical pathway. This indicates in this context activates...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSeparation and identification of carotenoids their oxidation products in the extracts human plasmaFrederick Khachik, Gary R. Beecher, Mudlagiri B. Goli, William Lusby, James C. Smith Jr.Cite this: Anal. Chem. 1992, 64, 18, 2111–2122Publication Date (Print):September 15, 1992Publication History Published online1 May 2002Published inissue 15 September...
ABSTRACT The Brachyury (T) gene is required for formation of posterior mesoderm and axial development in both mouse zebrafish embryos. In this paper, we first show that the Xenopus homologue Brachyury, Xbra, homologue, no tail (ntl), function as transcription activators. activation domains proteins map to their carboxy terminal regions, note domain absent two mutations, suggesting it function. A dominant-interfering Xbra construct was generated by replacing with repressor Drosophila...
Inductive interactions play a major role in the diversification of cell types during vertebrate development. These have been extensively studied amphibian embryos (usually Xenopus laevis) where earliest is mesoderm induction, which an equatorial mesodermal rudiment induced from animal hemisphere under influence signal vegetal hemisphere. The molecular basis induction unknown, although Tiedemann has isolated protein form 9- to 13-day chick that properties one would expect mesoderm-inducing...