A5023953483- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Redox biology and oxidative stress
- Nitric Oxide and Endothelin Effects
- Neuroinflammation and Neurodegeneration Mechanisms
- NF-κB Signaling Pathways
- Blood properties and coagulation
- Congenital heart defects research
- Immune cells in cancer
- Alzheimer's disease research and treatments
- Hippo pathway signaling and YAP/TAZ
- Genetics and Neurodevelopmental Disorders
- Monoclonal and Polyclonal Antibodies Research
- Mitochondrial Function and Pathology
- Biotin and Related Studies
- Peroxisome Proliferator-Activated Receptors
- Complement system in diseases
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Nanoparticle-Based Drug Delivery
- Nicotinic Acetylcholine Receptors Study
Otto-von-Guericke University Magdeburg
2020-2023
Leibniz Institute for Neurobiology
2016
Microglia, the resident immune cells of central nervous system (CNS), are derived from yolk-sac macrophages that populate developing CNS during early embryonic development. Once established, microglia population is self-maintained throughout life by local proliferation. As a scalable source microglia-like (MGLs), we here present forward programming protocol for their generation human pluripotent stem (hPSCs). The transient overexpression PU.1 and C/EBPβ in hPSCs led to homogenous mature...
Tauopathies are a major type of proteinopathies underlying neurodegenerative diseases. Mutations in the tau-encoding MAPT-gene lead to hereditary cases frontotemporal lobar degeneration (FTLD)-tau, which span wide phenotypic and pathological spectrum. Some these mutations, such as N279K mutation, result shift physiological 3R/4R ratio towards more aggregation prone 4R isoform. Other mutations V337M cause decrease vitro affinity tau microtubules reduced ability promote microtubule assembly....
Abstract Background Nanoparticles represent one of the most important innovations in medical field. Among nanocarriers, polymeric nanoparticles (PNPs) attracted much attention due to their biodegradability, biocompatibility, and capacity increase efficacy safety encapsulated drugs. Another improvement use as delivery systems is conjugation a targeting agent that enables accumulate specific tissue. Despite these advantages, clinical translation therapeutic approaches based on prevented by...
Abstract Cell death by glutamate excitotoxicity, mediated N -methyl- d -aspartate (NMDA) receptors, negatively impacts brain function, including but not limited to hippocampal neurons. The NF-κB transcription factor (composed mainly of p65/p50 subunits) contributes neuronal in while its inhibition should improve cell survival. Using the biotin switch method, subcellular fractionation, immunofluorescence, and luciferase reporter assays, we found that NMDA-stimulated activity selectively...
Abstract Learning and memory processes are accompanied by rearrangements of synaptic protein networks. While various studies have demonstrated the regulation individual proteins during these processes, much less is known about complex proteomes. Recently, we reported that auditory discrimination learning in mice associated with a relative down‐regulation involved structural organization synapses brain regions. Aiming at identification biological signaling pathways formation, here, label‐free...
The molecular synaptic mechanisms underlying auditory learning and memory remain largely unknown. Here, the workflow of a proteomic study on discrimination in mice is described. In this paradigm, are trained shuttle box Go/NoGo-task to discriminate between rising falling frequency-modulated tones order avoid mild electric foot-shock. protocol involves enrichment synaptosomes from four brain areas, namely cortex, frontal hippocampus, striatum, at different stages training. Synaptic protein...
Duplications and deletions of short chromosomal fragments are increasingly recognized as the cause for rare neurodevelopmental conditions disorders. The NDR2 gene encodes a protein kinase important neuronal development is part microduplication region on chromosome 12 that associated with intellectual disabilities, autism, epilepsy. We developed conditional transgenic mouse increased Ndr2 expression in postmigratory forebrain neurons to study consequences an dosage this Hippo pathway brain...
Abstract Cell death by glutamate excitotoxicity, mediated N-methyl-D-aspartate (NMDA) receptors, negatively impacts brain function, including but not limited to hippocampal neurons. The NF-κB transcription factor (composed mainly of p65/p50 subunits) contributes neuronal in while its inhibition should improve cell survival. Using the biotin switch method, subcellular fractionation, immunofluorescence and luciferase reporter assays, we found that NMDA stimulated activity selectively neurons,...
Translation initiation comprises complex interactions of eukaryotic factor (eIF) subunits and the structural elements mRNAs. is a key process for building cell’s proteome. It not only determines total amount protein synthesized but also controls translation efficiency individual transcripts, which important cancer or ageing. Thus, understanding during one that contributes to how eIF subunit composition influences other pathways yet attributed eIFs. We applied BioID technique two rapidly...