A5081893010- Malaria Research and Control
- Protein Structure and Dynamics
- Alzheimer's disease research and treatments
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Prion Diseases and Protein Misfolding
- Supramolecular Self-Assembly in Materials
- Sepsis Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Glycosylation and Glycoproteins Research
- Protein Kinase Regulation and GTPase Signaling
- Drug-Induced Hepatotoxicity and Protection
- Mosquito-borne diseases and control
- Hemodynamic Monitoring and Therapy
- Trypanosoma species research and implications
- RNA Research and Splicing
- Methemoglobinemia and Tumor Lysis Syndrome
- Histone Deacetylase Inhibitors Research
- Advanced Glycation End Products research
- Chromatography in Natural Products
- Endoplasmic Reticulum Stress and Disease
- Parasites and Host Interactions
- Infections and bacterial resistance
- Antibiotic Use and Resistance
Holy Family Hospital
2022-2024
Jamia Millia Islamia
2014-2023
National Institute of Malaria Research
2012-2021
Vardhman Mahavir Medical College & Safdarjung Hospital
2014-2021
Ionis Pharmaceuticals (United States)
2018-2020
Indian Council of Medical Research
2012
Rockefeller University
2010-2012
National Institute of Immunology
2011
Stony Brook University
2007-2010
State University of New York
2007-2009
There is considerable interest in uncovering the pathway of amyloid formation because toxic properties likely stems from prefibril intermediates and not fully formed fibrils. Using a recently invented method collecting 2-dimensional infrared spectra site-specific isotope labeling, we have measured development secondary structures for 6 residues during aggregation process 37-residue polypeptide associated with type 2 diabetes, human islet (hIAPP). By monitoring kinetics at different labeled...
Interactions of chemically modified nucleic acid therapeutics with plasma proteins play an important role in facilitating distribution from the injection site to peripheral tissues by reducing renal clearance. Despite importance these interactions, analytical methods that can characterize binding constants individual a reliable and high throughput manner are not easily available. We developed fluorescence polarization (FP) based assay measured for 25 most abundant human phosphorothioate (PS)...
Phosphorothioate-modified antisense oligonucleotides (PS-ASOs) interact with a host of plasma, cell-surface and intracellular proteins which govern their therapeutic properties. Given the importance PS backbone for interaction proteins, we systematically replaced anionic PS-linkages in toxic ASOs charge-neutral alkylphosphonate linkages. Site-specific incorporation alkyl phosphonates altered RNaseH1 cleavage patterns but overall rates activity versus on-target gene cells mice were only...
Amyloid formation has been implicated in a wide range of human diseases, and diverse set proteins is involved. There considerable interest elucidating the interactions which lead to amyloid contribute fibril stability. Recent attention focused upon potential role aromatic−aromatic aromatic−hydrophobic by short midsized polypeptides. Here we examine whether aromatic residues are necessary for islet polypeptide (IAPP). IAPP responsible type II diabetes thought play pathology disease. 37 length...
The 37-residue human islet amyloid polypeptide (hIAPP or amylin) self-assembles into fibers, the assembly of which has been associated with disease mechanism type II diabetes. Infrared spectroscopy in conjunction isotope labeling is proving to be a powerful tool for studying aggregation process hIAPP and other forming proteins residue specific structure kinetic information, but relationship between spectroscopic observables not fully established. We report detailed analysis linear 2D IR...
ABSTRACT Azithromycin is an effective treatment for uncomplicated infections with Salmonella enterica serovar Typhi and Paratyphi A (enteric fever), but there are no clinically validated MIC disk zone size interpretative guidelines. We studied individual patient data from three randomized controlled trials (RCTs) of antimicrobial in enteric fever Vietnam, azithromycin used one arm, to determine the relationship between response infecting isolate. additionally compared susceptibility sizes...
Conjugation of antisense oligonucleotide (ASO) with a variety distinct lipophilic moieties like fatty acids and cholesterol increases ASO accumulation activity in multiple tissues. While lipid conjugation tissue exposure mice reduces excretion urine, histological review skeletal cardiac muscle indicates that the increased conjugated is isolated to interstitium. Administration palmitic acid-conjugated (Palm-ASO) results rapid substantial interstitium followed by relatively clearance only...
Objective To describe India's National Antimalarial Drug Resistance Monitoring System, measure the efficacy of first-line malaria treatments, and determine risk factors for treatment failure.Methods In 2009-2010, prospective studies with 28 days follow-up were conducted at 25 sentinel sites.Patients infected Plasmodium falciparum given artesunate plus sulfadoxine-pyrimethamine (AS+SP); those P. vivax chloroquine.Polymerase chain reaction was used to distinguish post-treatment reinfection...
Graphical Abstract Subtle ways: Analogues of amyloid-forming polypeptides have been developed by using p-cyanophenylalanine as a sensitive, noninvasive, intrinsic probe amyloid formation. p-Cyanophenylalanine (see figure) is very conservative substitution for phenylalanine or tyrosine, and its fluorescence incredibly sensitive to the environment; these facets make it powerful
Sepsis is a global health problem with high morbidity and mortality. Low- middle-income countries have higher incidence poorer outcome sepsis. Large epidemiological studies in sepsis using Sepsis-3 criteria, addressing the process of care deriving predictors mortality are scarce India. A multicentre, prospective registry was conducted 3 criteria suspected or confirmed infection SOFA score 2 more 19 ICUs India over period one year (August 2022–July 2023). All adult patients admitted to...
Nucleobindin 1 (NUCB1) is a widely expressed multidomain calcium-binding protein whose precise physiological and biochemical functions are not well understood. We engineered heterologously soluble form of NUCB1 (sNUCB1) characterized its biophysical properties. show that sNUCB1 exists as dimer in solution each monomer binds two divalent calcium cations. Calcium binding causes conformational changes judged by circular dichroism fluorescence spectroscopy experiments. Earlier reports suggested...
Sepsis is associated with wide variable coagulation abnormalities. Thromboelastography (TEG) effectively measures the viscoelastic properties of clots. This study aims to illustrate clot quality and mass in sepsis septic shock patients using TEG, as an effective tool over standard tests.A single-center, prospective observational was conducted. 50 each meeting criteria for shock, a healthy group 30 included study. Blood samples were obtained analyzed tests, platelet count, fibrinogen, TEG...
Widespread resistance to chloroquine was the mainstay implement artemisinin-based combination therapy (ACT) in year 2007 few malaria endemic states India including Jharkhand as first line of treatment for uncomplicated Plasmodium falciparum malaria. This study conducted state country just after implementation ACT assess prevailing antimalarial drug prescribing practices, availability drugs and acceptability new policy by health professionals P. patients particularly children ≤ 15 yr age.This...
The C-terminal domain of ribosomal protein L9 (CTL9) is a 92-residue alpha-beta which contains an unusual three-stranded mixed parallel and antiparallel beta-sheet. folds in two-state fashion, the folding rate slow. It thought that slow may be caused by necessity forming this beta-sheet architecture transition state for folding. This hypothesis makes CTL9 interesting target studies. was characterized phi-value analysis. set hydrophobic core mutants analyzed together with truncation mutants....
To establish the relative importance of Salmonella enterica serovar Typhi with non-classical quinolone resistance. Eight hundred and ninety-one isolates S. Typhi, isolated between 2004 2011, were tested for antibiotic susceptibility determination using disc diffusion E-test. The mechanisms fluoroquinolone resistance studied in a sub-set NALS (nalidixic acid susceptible) by wave nucleic fragment analysis PCR products from gyrA, gyrB, parC parE plasmid borne determinants: qnrA,B,S;...
High temperatures present a formidable challenge to the cultivation of hot pepper, profoundly impacting not only vegetative growth but also leading flower and fruit abscission, thereby causing significant reduction in yield. To unravel intricate genetic mechanisms governing heat tolerance an F 2 population was developed through crossing two distinct genotypes exhibiting contrasting characteristics: DLS-161-1 (heat tolerant) DChBL-240 susceptible). The population, along with parental lines,...
Fluoroquinolone resistance is mediated by mutations in the quinolone-resistance determining region (QRDR) of topoisomerase genes. Denaturing high performance liquid chromatography (DHPLC) was evaluated for detection clinically important gyrB among Salmonella. Salmonella Typhi and S. Paratyphi A characterised mutation QRDR gyrA, parC parE were studied DHPLC validated sequencing. The analysis able to resolve test mutant from isolates with wild type distinguished mutants other peak profile...
Tyrosinase (polyphenolic oxidase) is a multifunctional and copper containing enzyme. an enzyme which responsible for melanin biosynthesis color of the skin. Melanin synthesised in melanocyte cells by melanogenesis process. Melanogenesis protects skin surface from harmful ultraviolet radiations. mainly synthesized plants, micro organisms mammalian cells. pigment hyperpigmentation hypopigmentation. When present very less amount it causes local vitiligo posttraumatic Abnormal deposit specific...
Background Minisatellites are an integral part of eukaryotic genomes and show variation in the complexity their organization. Besides presence non-coding regions, a small fraction them transcriptome, possibly participating gene regulation, expression silencing. We studied minisatellite (TGG)n tagged transcriptome water buffalo Bubalus bubalis across various tissues spermatozoa, characterized genes TSPY1 COL6A1 discovered process. Results Minisatellite associated sequence amplification (MASA)...