A5040368039- Hepatitis B Virus Studies
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Hepatitis C virus research
- Cardiac Imaging and Diagnostics
- Pancreatic function and diabetes
- Cancer, Lipids, and Metabolism
- Cardiovascular Disease and Adiposity
- Diabetes and associated disorders
- Cardiac Structural Anomalies and Repair
- Hepatitis Viruses Studies and Epidemiology
- Cardiovascular Function and Risk Factors
- Lipid metabolism and disorders
- Coronary Interventions and Diagnostics
- Neurofibromatosis and Schwannoma Cases
- Meningioma and schwannoma management
- Cardiovascular Health and Disease Prevention
- Adipose Tissue and Metabolism
- Cervical Cancer and HPV Research
- Ion channel regulation and function
- Atherosclerosis and Cardiovascular Diseases
- Cardiac Ischemia and Reperfusion
- Metabolism, Diabetes, and Cancer
- Adipokines, Inflammation, and Metabolic Diseases
Saint Barnabas Medical Center
2018-2024
Capital Medical University
2011-2024
Hepatitis B Foundation
2024
Third People's Hospital of Hangzhou
2024
Zhejiang University
2021-2024
First Affiliated Hospital Zhejiang University
2021-2024
Second Xiangya Hospital of Central South University
2024
Central South University
2024
University of Science and Technology of China
2024
Nanjing Medical University
2024
The reduction in podocyte density to levels below a threshold value drives glomerulosclerosis and progression ESRD. However, technical demands prohibit high-throughput application of conventional morphometry for estimating density. We evaluated method using single paraffin-embedded formalin-fixed sections. Podocyte nuclei were imaged indirect immunofluorescence detection antibodies against Wilms' tumor-1 or transducin-like enhancer split 4. To account the large size relation section...
DNA vaccines encoding for murine rotavirus proteins VP4, VP6, or VP7 were tested in adult BALB/cmice their ability to induce immune responses and protect against challenge. A gene gun was used inoculate into the epidermis. Rotavirus-specific serum antibodies, as measured by ELISA, virus-specific cytotoxicT lymphocyte generated each of three vaccines, but virus-neutralizing antibodies detected only mice that with VP4 VP7. Efficacy determined challenge 100 ID50 homotypic rotavirus. Each...
Three weeks after myocardial infarction (MI) in the rat, remodeled hypertrophy of noninfarcted myocardium is at its maximum and heart a compensated stage with no evidence failure. Our hemodynamic measurements this showed slight but insignificant decrease +dP/dt significantly higher left ventricular end-diastolic pressure. To investigate basis diastolic dysfunction, we explored possible defects beta-adrenergic receptor-G(s/i) protein-adenylyl cyclase-cAMP-protein kinase A-phosphatase pathway,...
Abstract Purpose: Aberrant arachidonic acid (AA) metabolism, especially through the cyclooxygenase (Cox) and 5-lipoxygenase (5-Lox) pathways, has been suggested to play an important role in development of esophageal adenocarcinoma (EAC). The purpose this study was investigate expression 5-Lox EAC a rat model human samples as well chemopreventive effects zileuton (a specific inhibitor) celecoxib Cox2 model. Experimental Design: esophagogastroduodenal anastomosis humans examined with...
Norwalk virus, an important cause of epidemic, acute, nonbacterial gastroenteritis in adults and children, has eluded adaptation to tissue culture, the development animal model, molecular cloning. In this study, a portion viral genome encoding immunoreactive region was cloned from very small quantities infected stool using sequence-independent single primer amplification. Six overlapping complementary DNA (cDNA) clones were isolated by immunologic screening. The expressed recombinant protein...
Hyperinsulinemia is commonly viewed as a compensatory response to insulin resistance, yet studies have demonstrated that chronically elevated may also drive resistance. The molecular mechanisms underpinning this potentially cyclic process remain poorly defined, especially on transcriptome-wide level. Transcriptomic meta-analysis in >450 human samples fasting reliably and negatively correlated with INSR mRNA skeletal muscle. To establish causality study the direct effects of prolonged...
Fibroblast growth factor 1 (FGF1) has been recently characterized as a potent insulin sensitizer that regulates adipose tissue remodeling, but the physiological role of FGF1 remains unclear. This study measured serum levels for first time in patients with newly diagnosed type 2 diabetes mellitus (T2DM), and further explored correlations between various metabolic parameters T2DM. Serum were determined using ELISA age-, sex- BMI- matched subjects normal glucose tolerance (NGT) (n=80) T2DM...
This study was designed to explore the protective effect of 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside (TSG) against ischemia/reperfusion (I/R) injury-induced cardiomyocytes apoptosis.The H9c2 cell I/R injury model induced by simultaneous shortage nutrients and oxygen. TSG administration (0.10, 0.25, 0.50 mM) performed before during stimulation. Cell apoptosis evaluated using terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Expression cell-related proteins detected...
Mice over-expressing the alpha(1)_subunit (pore) of L-type Ca2+ channel (alpha(1C)TG) by 4 months (mo) age exhibit an enlarged heart, hypertrophied myocytes, increased current and transient amplitude, but a normal SR load. With advancing (8-11 mo), some mice demonstrate advanced hypertrophy are not in congestive heart failure (NFTG),while others evolve to frank dilated (FTG). We that older NFTG myocytes hypercontractile state over wide range stimulation frequencies, maintain load compared...
Five unprecedented chromone derivatives involving a 6/6/5/5/5/6 hexacyclic scaffold (1, 2), 6/6/5/6/6/6/6 heptacyclic (3), and 6/6/6/5/5/6 (4, 5) were obtained from the fungus Aspergillus deflectus NCC0415. Their structures identified using comprehensive spectroscopic analysis, single-crystal X-ray diffraction, electronic circular dichroism calculations. Except for 3, other compounds, especially (4 5), exhibited potent inosine-5′-monophosphate dehydrogenase (IMPDH) inhibitory activities.
The expression profile of miRNAs and their function in condyloma acuminatum (CA) remains unknown. In this study, we aimed to detect the effects miR-143 miR-145, most downregulated CA samples using high-throughput sequencing, on cell proliferation apoptosis, determine a novel therapeutic target for recurrence. RT-qPCR was used validate lower miR-145 larger size samples, NRAS significantly higher than self-controls as determined by western blotting assay. Luciferase assay performed confirm...
The aim of this study was to evaluate the efficacy intracranial aneurysm treatment with help neuroendoscope.Eighty-eight patients were treated from February 2000 November 2003 for aneurysms which 89 lesions clipped neuroendoscope, including 82 anterior circulation (in 81 cases) and 7 posterior aneurysms. diameters between 5 40 mm mean value 12.5 mm. In Hunt Hess preoperative classification, 10 cases grade 0, 37 I, 36 II, III.Postoperative complications observed in (7.9%), hemiplegia (1 case...
Objectives. To measure disparities in hepatitis B virus (HBV) infection and immunity among a high-risk patient population at community health center New York City. Methods. We performed retrospective chart review of 25 565 adults with HBV surface antigen, antibody, total core antibody tests from 1997 to 2017. categorized test results by status analyzed demographic characteristics χ 2 logistic regression analysis. Results. Of adults, 13.4% were currently infected, 52.1% ever 33.4% immune...
This case report describes tofacitinib treatment for 2 patients with pretibial myxedema.
Introduction: MDM2 is known as the primary negative regulator of p53, promotes lung cancer fibrosis and injury through p53-dependent p53-independent pathways. However, mechanism by which influences pathogenesis asthma unknown. In this study, we investigated function in epithelial cells type 2 inflammation. Methods: We used II alveolar cell-specific heterozygous knockout Mdm2 mice to validate its function. Then papain-induced model was established, changes inflammation were observed measuring...
Abstract: Prurigo nodularis (PN) is an intense pruritic skin condition. Treatment of PN challenging. We described elderly patient with who had contradictions cyclosporine or methotrexate and achieved significant improvement after treatment dupilumab. also reviewed published cases patients were refractory to traditional therapy. Keywords: prurigo nodularis, dupilumab, patient, targeted therapy