A5103237707- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- Cytokine Signaling Pathways and Interactions
- Genetics, Aging, and Longevity in Model Organisms
- interferon and immune responses
- Lipid Membrane Structure and Behavior
- Ovarian cancer diagnosis and treatment
- Cancer Mechanisms and Therapy
- Viral Infectious Diseases and Gene Expression in Insects
- Vitamin C and Antioxidants Research
- Uterine Myomas and Treatments
- Antimicrobial Peptides and Activities
- Complement system in diseases
- Biochemical effects in animals
- Endometrial and Cervical Cancer Treatments
- Protein purification and stability
Korea Advanced Institute of Science and Technology
2014-2020
The University of Texas Health Science Center at San Antonio
1990
Interleukin-6 (IL-6) is a multifunctional cytokine that regulates immune responses for host defense and tumorigenic process. Upregulation of IL-6 known to constitutively phosphorylate signal transducer activator transcription 3 (STAT3), leading activation multiple oncogene pathways inflammatory cascade. Here, we present the development high-affinity protein binder, termed repebody, which effectively suppresses non–small cell lung cancer in vivo by blocking IL-6/STAT3 signaling. We selected...
The importance of a downstream process for the purification immunoglobulin antibodies is increasing with growing application monoclonal in many different areas. Although protein A most commonly used affinity antibodies, certain properties could be further improved: higher stability alkaline solution and milder elution condition. Herein, we present development Fc-specific repebody by modular engineering approach its potential as an ligand human antibodies. We previously developed scaffold...
The major difficulty in defining the mechanisms for action of food restriction relates to its diversity and lack specificity. In searching common factor(s) commonality involved such diversified effects, a cellular homeostasis mechanism is proposed. A study initiated our laboratory strongly indicates that homeostatic seriously compromised by oxidative damage free radical reaction with aging. Data on mitochondrial hydroperoxide, microsomal cytochrome P-450 breakdown, reduction cytosolic...
Precise binding mode identification and subsequent affinity improvement without structure determination remain a challenge in the development of therapeutic proteins. However, relevant experimental techniques are generally quite costly, purely computational methods have been unreliable. Here, we show that integrated epitope localization followed by full-atom energy minimization can yield an accurate complex model which ultimately enables effective redesign specificity. As proof-of-concept,...
Abstract Precise binding mode identification and subsequent affinity improvement without structure determination remain a challenge in the development of therapeutic proteins. However, relevant experimental techniques are generally quite costly, purely computational methods have been unreliable. Here, we show that integrated epitope localization followed by full-atom energy minimization can yield an accurate complex model which ultimately enables effective redesign specificity. As...