A5073688909- Genetic and phenotypic traits in livestock
- Epigenetics and DNA Methylation
- Genetic Mapping and Diversity in Plants and Animals
- Genetic Syndromes and Imprinting
- Prenatal Screening and Diagnostics
- Animal Nutrition and Physiology
- Animal Genetics and Reproduction
- Rabbits: Nutrition, Reproduction, Health
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Agriculture and Rural Development Research
- melanin and skin pigmentation
- Genetics, Bioinformatics, and Biomedical Research
- Nutrition, Genetics, and Disease
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Gut microbiota and health
- Genetic diversity and population structure
- Animal Virus Infections Studies
- Reproductive Biology and Fertility
- Biochemical Analysis and Sensing Techniques
- Organ Transplantation Techniques and Outcomes
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
Génétique Physiologie et Systèmes d'Elevage
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Département Génétique Animale
2020-2025
École Nationale Vétérinaire de Toulouse
2013-2025
Université de Toulouse
2017-2024
Institut National Polytechnique de Toulouse
2017-2022
École Nationale Supérieure Agronomique de Toulouse
2022
Baker Heart and Diabetes Institute
2009-2014
Laboratoire de Génétique Cellulaire
2004-2013
Laboratoire de Génie Chimique
2012-2013
Some sheep breeds are naturally prolific, and they very informative for the studies of reproductive genetics physiology. Major genes increasing litter size (LS) ovulation rate (OR) were suspected in French Grivette Polish Olkuska populations, respectively. To identify genetic variants responsible highly prolific phenotype these two breeds, genome-wide association (GWAS) followed by complementary functional analyses performed. Highly ewes (cases) normal (controls) from each breed genotyped...
The imprinted expression of the IGF2 and H19 genes is controlled by imprinting control region 1 (ICR1) located at chromosome 11p15.5. This methylation-sensitive chromatin insulator works binding zinc-finger protein CTCF in a parent-specific manner. DNA methylation defects involving ICR1 H19/IGF2 domain result two growth disorders with opposite phenotypes: an overgrowth disorder, Beckwith-Wiedemann syndrome (maternal gain 10% BWS cases) retardation Silver-Russell (paternal loss 60% SRS...
The composition and structure of fleece variation observed in mammals is a consequence strong selective pressure for fiber production after domestication. In sheep, discriminates ancestral species carrying long hairy from modern domestic sheep (Ovis aries) owning short woolly fleece. Here, we report that the “woolly” allele results insertion an antisense EIF2S2 retrogene (called asEIF2S2) into 3′ UTR IRF2BP2 gene leading to abnormal transcript. We provide evidence this chimeric...
The imprinted 11p15 region is organized in two domains, each of them under the control its own imprinting (ICR1 for IGF2/H19 domain and ICR2 KCNQ1OT1/CDKN1C domain). Disruption results fetal growth disorders with opposite phenotypes: Beckwith–Wiedemann (BWS) Silver–Russell (SRS) syndromes. Various genetic epigenetic defects have been demonstrated BWS SRS. Among them, isolated DNA methylation account approximately 60% patients. To investigate whether cryptic copy number variations (CNVs)...
Genomic imprinting, a mechanism resulting in parent-of-origin expression of genes through epigenetic regulation, intersects with broad range biological fields including evolution, molecular genetics and epigenetics determinism complex traits. Although next generation sequencing technologies enable nowadays to detect imprinted genome-wide manner, wide spectrum this phenomena is evaluated only humans mice. Here, we propose map showing parental bias hypothalamus, muscle placenta piglets around...
Summary The ability of chickens to carry Salmonella without displaying disease symptoms is responsible for propagation in poultry stocks and subsequent human contamination through the consumption contaminated eggs or meat. selection animals more resistant carrier state might be a way decrease its transmission humans. Five QTL controlling variation resistance chicken F 2 progeny derived from White Leghorn inbred lines N 6 1 had been previously identified using selective genotyping approach....
Abstract Genomic imprinting represents an original model of epigenetic regulation resulting in a parent-of-origin expression. Despite the critical role imprinted genes mammalian growth, metabolism and neuronal function, there is no molecular tool specifically targeting them for systematic evaluation. We show here that enzymatic methyl-seq consistently outperforms bisulfite-based standard capturing 165 candidate regions genomic pig. This highlights potential turnkey, fully customizable...
Coat color dilution corresponds to a specific pigmentation phenotype that leads of wild type pigments. It affects both eumelanin and pheomelanin containing melanosomes. The mode inheritance the is autosomal recessive. Candidate gene approaches focused on melanophilin (MLPH) highlighted two variants associated with in rabbits: c.111-5C>A variant located an acceptor splice site or c.585delG variant, frameshift mutation. On transcript level, skipping exons has been reported as molecular...
The sequencing of total RNA depleted for ribosomal sequences remains the method choice study circRNAs. Our objective was to characterize non-canonical circRNAs, namely not originating from back splicing and circRNA produced by non-coding genes. To this end, we analyzed a dataset porcine testis known contain about 100 intron-derived Labelling reads containing circular junction provided information on very small contribution long genes production canonical Analyses other revealed two origins...
Abstract Background New preservation solutions are emerging, of various ionic compositions and with hydroxyethyl starch replaced by polymers such as polyethylene glycols (PEGs), offering the potential for ‘immunocamouflage’. This experimental study investigated which three clinically available protocols offered best graft protection, based on epithelial-to-mesenchymal transition (EMT) fibrosis. Methods Kidneys were preserved 24 h at 4 °C University Wisconsin solution (UW) standard, compared...
Fetal growth is a complex process depending on the genetics of fetus, availability nutrients to maternal nutrition and various factors hormones maternal, fetal placental origin. The IGF system, more particularly IGF2, one most important endocrine paracrine systems regulating (reviewed in [1]). IGF2 gene regulated by genomic imprinting expressed only from paternally-inherited allele tissues during development after birth. Imprinted genes are tightly therefore susceptible changes, including...
Pig chromosome 7 (SSC 7) has been shown to be rich in QTL affecting performance and quality traits. Most studies mapped the close swine leukocyte antigens (SLA), which a large effect on adaptability natural selection. Previous comparative mapping suggested that 15-cM region limited by markers LRA1 (mapped at 55 cM) S0102 70 contains hundreds of genes. To decrease number candidate genes, we improved resolution with genetic dissection through backcross recombinant progeny test program between...
Abstract Background On porcine chromosome 7, the region surrounding Major Histocompatibility Complex (MHC) contains several Quantitative Trait Loci (QTL) influencing many traits including growth, back fat thickness and carcass composition. Previous studies highlighted that a fragment of ~3.7 Mb is located within Swine Leucocyte Antigen (SLA) complex. Internal rearrangements this were suggested, partial contigs had been built, but further characterization identification all human chromosomal...
<h3>Background</h3> Disruption of 11p15 imprinting results in two fetal growth disorders with opposite phenotypes: the Beckwith–Wiedemann (BWS; MIM 130650) and Silver–Russell (SRS; 180860) syndromes. DNA methylation defects account for 60% BWS SRS cases and, most cases, occur without any identified mutation a <i>cis-</i>acting regulatory sequence or <i>trans</i>-acting factor. <h3>Methods</h3> We investigated whether variants primary patients loss at ICR1 ICR2, respectively. <h3>Results</h3>...
Feed efficiency is a major production trait in animal genetic breeding schemes. To further investigate the control of feed rabbits, we performed genome-wide association study (GWAS) for growth and on 679 rabbits genotyped with Affimetrix Axiom Rabbit 200K Genotyping Array. After quality control, 127 847 single-nucleotide polymorphisms (SNP) were retained analyses. The GWAS using GEMMA software, applying mixed univariate model linear regression each SNP allele. traits analysed weight at...
Circular intronic RNAs (ciRNAs) are still unexplored regarding mechanisms for their emergence. We considered the ATXN2L intron lariat-derived circular RNA (ciRNA-ATXN2L) as an opportunity to conduct a cross-species examination of ciRNA genesis. To this end, we investigated 207 datasets from 4 tissues and 13 mammalian species. While in eight species, ciRNA-ATXN2L was never detected, pigs rabbits, expressed all sometimes at very high levels. Bovine were intermediate case macaques cats, only...
Renal failure due to ischemic injury is a common denominator of various clinical situations in critically ill patients. This study was designed characterize the TPSO/Cholesterol synthesis and cell division pathways response different levels ischemia. Porcine kidneys were subjected either 60 min-warm ischemia (WI) or auto-transplanted after cold storage for 24 h at 4°C (CS), both conditions (WI+CS), pathway activation function evaluated 3 h, 7 days reperfusion. CS combined WI affects renal...