A5076590763- Glycosylation and Glycoproteins Research
- Trypanosoma species research and implications
- Carbohydrate Chemistry and Synthesis
- Cystic Fibrosis Research Advances
- Drug Transport and Resistance Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
University of Zurich
2017
ETH Zurich
2017
The biosynthesis of eukaryotic lipid-linked oligosaccharides (LLOs) that act as donor substrates in protein N-glycosylation starts on the cytoplasmic side endoplasmic reticulum and includes sequential addition five mannose units to dolichol-pyrophosphate-GlcNAc2. These reactions are catalyzed by Alg1, Alg2 Alg11 gene products yield Dol-PP-GlcNAc2Man5, an LLO intermediate is subsequently flipped lumen reticulum. While purification active Alg1 has previously been described, have mostly studied...
The initial transfer of a complex glycan in protein N-glycosylation is catalyzed by oligosaccharyltransferase (OST), which generally multisubunit membrane the endoplasmic reticulum but single-subunit enzyme (ssOST) some protists. To investigate reaction mechanism ssOST, we recombinantly expressed, purified and characterized STT3A from Trypanosoma brucei (TbSTT3A). We analyzed vitro activity TbSTT3A synthesizing fluorescently labeled acceptor peptides as well lipid-linked oligosaccharide...
Abstract PglK is an ABC transporter that flips a lipid-linked oligosaccharide (LLO) serves as donor in protein N-glycosylation. Previous structures revealed two inward-facing conformations, both with very large separations of the nucleotide binding domains (NBDs), and closed, ADP-bound state featured occluded cavity. To investigate additional states, we developed conformation-sensitive, single-domain camelid nanobodies (Nb) studied their effect on activity. Biochemical, structural, mass...
ABSTRACT The Burkholderia cepacia complex (Bcc) displays a wealth of metabolic diversity with great biotechnological potential, but the utilization these bacteria is limited by their opportunistic pathogenicity to humans. third replicon Bcc, megaplasmid pC3 (0.5 1.4 Mb, previously chromosome 3), important for various phenotypes, including virulence, antifungal, and proteolytic activities certain substrates. Approximately half plasmid well conserved throughout sequenced Bcc members, while...