A5091315259- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- RNA modifications and cancer
- Plant biochemistry and biosynthesis
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- Synthetic Organic Chemistry Methods
- Advanced Proteomics Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Coordination Chemistry and Organometallics
- Signaling Pathways in Disease
- Genomics and Phylogenetic Studies
- Inflammasome and immune disorders
- Pancreatic function and diabetes
- Lanthanide and Transition Metal Complexes
- Cancer-related Molecular Pathways
- Cellular transport and secretion
- Click Chemistry and Applications
- Marine Sponges and Natural Products
- Lipid metabolism and biosynthesis
- Bone health and treatments
- Bone Metabolism and Diseases
MRC Laboratory of Molecular Biology
2016-2018
Medical Research Council
2016-2018
The University of Queensland
2010-2017
Incorporation of unnatural amino acids (uAAs) via codon reassignment is a powerful approach for introducing novel chemical and biological properties to synthesized polypeptides. However, the site-selective incorporation multiple uAAs into polypeptides hampered by limited number reassignable nonsense codons. This challenge addressed in current work developing Escherichia coli vitro translation system depleted specific endogenous tRNAs. The translational activity this dependent on addition...
A marine-derived actinomycete, Nocardiopsis sp. (CMB-M0232), obtained from a sediment sample collected at depth of 55 m off the coast Brisbane, Australia, yielded two new macrolide polyketides. Structures for nocardiopsins and B were assigned by detailed spectroscopic analysis, degradation chemical derivatization. Marfey's analysis revealed an unexpected acid-mediated partial racemization L-pipecolic acid incorporated within nocardiopsins. The scope this was assessed against selection...
Genetic code expansion is a key objective of synthetic biology and protein engineering. Most efforts in this direction are focused on reassigning termination or decoding quadruplet codons. While the redundancy genetic provides large number potentially reassignable codons, their utility diminished by inevitable interaction with cognate aminoacyl-tRNAs. To address problem, we sought to establish an vitro synthesis system simplified tRNA complement, thereby orthogonalizing some sense This...
Bisphosphonate drugs such as zoledronic acid (ZOL), used for the treatment of common bone disorders, target skeleton and inhibit resorption by preventing prenylation small GTPases in bone-destroying osteoclasts. Increasing evidence indicates that bisphosphonates also have pleiotropic effects outside skeleton, most likely via cells monocyte/macrophage lineage exposed to nanomolar circulating drug concentrations. However, no low concentrations ZOL been reported using existing approaches. We...
Abstract Protein modification with isoprenoid lipids affects hundreds of signaling proteins in eukaryotic cells. Modification isoprenoids reporter groups is the main approach for creation probes analysis protein prenylation vitro and vivo. Here, we describe a new strategy synthesis functionalized phosphoisoprenoids that uses an aminederivatized scaffold as starting point phosphoisoprenoid libraries. This overcomes long‐standing problem field, where multistep had to be carried out each...
Vesicular transport of cellular cargo requires targeted membrane fusion and formation a SNARE protein complex that draws the two apposing fusing membranes together. Insulin-regulated delivery glucose transporter-4 storage vesicles at cell surface is dependent on key proteins: integral Syntaxin4 (Sx4) soluble regulatory Munc18c. Many reported in vitro studies Munc18c:Sx4 interactions have used Sx4 constructs lacking native transmembrane domain. As consequence, importance C-terminal anchor...