A5019287469- Marine Sponges and Natural Products
- Advanced biosensing and bioanalysis techniques
- Microbial Natural Products and Biosynthesis
- RNA Interference and Gene Delivery
- Advanced oxidation water treatment
- Analytical chemistry methods development
- Synthesis and Biological Activity
- Chemical synthesis and alkaloids
- Synthetic Organic Chemistry Methods
- Nanoplatforms for cancer theranostics
- Biochemical Analysis and Sensing Techniques
- Metal-Catalyzed Oxygenation Mechanisms
- Electrochemical Analysis and Applications
- Icing and De-icing Technologies
- Metal and Thin Film Mechanics
- Alkaloids: synthesis and pharmacology
- Polysaccharides and Plant Cell Walls
- MicroRNA in disease regulation
- Nanoparticle-Based Drug Delivery
- RNA and protein synthesis mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant biochemistry and biosynthesis
- ZnO doping and properties
- Microbial bioremediation and biosurfactants
- EEG and Brain-Computer Interfaces
Jiangnan University
2021-2025
Central South University
2017-2024
Gifu University
2024
University of Southern California
2021-2024
Tianjin Institute of Industrial Biotechnology
2024
Chinese Academy of Sciences
2024
Shizuoka University
2024
Beijing Institute of Aeronautical Materials
2024
Harbin Engineering University
2023-2024
Southern Medical University
2021-2024
Direct, rapid, sensitive, and selective detection of nucleic acids in complex biological fluids is crucial for medical early diagnosis. We herein combine the trans-cleavage ability clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a with Au-nanobeacon to establish a CRISPR-based biosensor, providing rapid miRNA high speed attomolar sensitivity. In this strategy, we first report that activity CRISPR/cas12a, which was previously reported be triggered only by target ssDNA...
Radiation therapy plays a crucial role in cancer treatment, necessitating precise delivery of radiation to tumors while sparing healthy tissues over multiple days. Computed tomography (CT) is integral for treatment planning, offering electron density data accurate dose calculations. However, accurately representing patient anatomy challenging, especially adaptive radiotherapy, where CT not acquired daily. Magnetic resonance imaging (MRI) provides superior soft-tissue contrast. Still, it...
Abstract Plant-derived alkaloids are an important class of pharmaceuticals. However, they still rely on phytoextraction to meet their diverse market demands. Since multistep biocatalytic cascades have begun revolutionize the manufacture natural or unnatural products, address synthetic challenges alkaloids, herein we establish artificially concise four-enzyme cascade with avoiding plant-derived P450 modification for synthesizing phenethylisoquinoline (PEIAs) after enzyme discovery and...
Steviol glycosides (SGs) are highly valued for their sweetness, safety, and zero calories, but bitter taste low solubility limit application. Modifying glycosyl units is a promising strategy to improve sensory qualities. In this study, we identified the enzyme UGT94E13 through phylogenetic analysis screening, which catalyzes glycosylation of rebaudioside M2 (Reb M2) at C-13 position, producing novel β-1,6-O-glycosylated product M9 M9). Subsequently, catalytic activity toward Reb was enhanced...
Owing to zero-calorie and advanced organoleptic properties similar sucrose, the plant-derived rebaudioside M (Reb M) has been considered as a next generation sweetener. However, low content of Reb in Stevia rebaudiana Bertoni enzymatic activity UGT76G1, which is an uridine diphosphate glucose (UDPG)-dependent glycosyltransferase with ability glycosylate D D) produce through formation β-1,3 glycosidic bond, restrict its commercial usage. To improve catalytic variant UGT76G1-T284S/M88L/L200A...
New-to-nature biocatalysis in organic synthesis has recently emerged as a green and powerful strategy for the preparation of valuable chiral products, among which oxygen-containing benzo-fused heterocycles are important structural motifs pharmaceutical industry. However, asymmetric these compounds through radical-mediated methods is challenging. Herein, novel photoenzymatic developed to realize efficient enantioselective structure-guided engineering flavin-dependent 'ene'-reductase GluER. It...
Abstract The construction of structural complexity and diversity natural products is crucial for drug discovery development. To overcome high dark toxicity poor photostability photosensitizer perylenequinones (PQs) photodynamic therapy, herein, we aim to introduce the biosynthesize desired unnatural PQs in fungus Cercospora through synthetic biology‐based strategy. Thus, first elucidate intricate biosynthetic pathways class B reveal how branching enzymes create their from a common ancestor....
An atypical nonheme iron-dependent dioxygenase BTG13 with a rare iron coordination of four histidine residues and carboxylated-lysine (Kcx) was recently reported to catalyze the C4a–C10 bond cleavage anthraquinone. However, reaction mechanism remains elusive. Herein, detailed is studied using molecular dynamics simulations density functional theory calculations. The comprehensive mechanistic study shows that most favorable pathway for C–C anthraquinone involves two unusual steps: (1)...
The grain boundary phase of Dy-free sintered Nd-Fe-B magnets is modified by using Pr68Cu32 eutectic alloy. coercivity the reaches 21 kOe, which highest value in magnets. Microstructural investigations show that a smooth and thick layer formed, content ferromagnetic elements decreases from 65 at. % to 9 %. In addition, mean size (4.5 μm) doped smaller than (6.5 original modification reduces magnetic interactions among grains hinders nucleation reversed domains, resulting enhancement.
Deep neural networks (DNNs) have the powerful ability to automatically extract efficient features, which makes them prominent in electroencephalogram (EEG) based seizure prediction tasks. However, current research this field cannot take model uncertainty into account, causing less credible. To end, we introduce a novel end-to-end patient-specific framework via learning. Specifically, propose reparameterized EEG-based lightweight CNN architecture and modified Monte Carlo dropout (RepNet-MMCD)...
The precise detection of extracellular ATP, although a challenging task, is great significance for understanding the related cell processes. Herein, we developed ratiometric DNA nanoswitch by employing tweezer and split aptamer. composed three specially designed ssDNA strands, namely, central strands O1, O2, O3. This can be anchored on membrane cholesterol labeled at 3' end Initially, adopts an open state, separating dual fluorophores giving rise to low FRET (fluorescence resonance energy...
We, herein, indicated for the first time Rnase activities of LbCas12a on linear ssRNA above 11 bases, and hairpin RNA substrates. Meanwhile, bound to ssDNA or exhibited different cleavage efficiencies various substrates, including short ssDNA, DNA, RNA. With DNA as a reporter, we attained detection limit 5 pM 50 targets, respectively. We believe that these findings will pave new avenue expanding reporter toolbox Cas12a-based diagnostics in biosensing biochemistry.
Methylenedioxy bridges (MDBs) are architecturally important motifs in natural products and bioactive molecules. Cercosporin, a typical perylenequinone pigment, contains an unusual seven-membered MDB, which has versatile biological photocatalytic activities. Although cercosporin been isolated, characterized, studied for several decades, its biosynthetic pathway, especially the formation of remained unclear. Here, we show that MDB is catalyzed by Fe(II)/α-ketoglutaric acid (α-KG)-dependent...
In this work, we present a highly sensitive, specific, and versatile method to quantify miRNA expression by coupling CRISPR-Cas12a with cyclic reverse transcription (CRT), termed as CRISPR-CRT. Each target was first converted amplified into multiple hairpin RT products via CRT. Afterward, the could serve activators initiate collateral cleavage activity of CRISPR-Cas12a. Due above two-stage amplification, assay detect at sub-femtomolar level (LOD, 0.201 fM). Since sequence is double checked:...
Plant-derived exosome-like nanovesicles carry a variety of bioactive substances and have multiple health benefits. Pomegranate (Punica granatum) is popular fruit with hepatoprotective effects. However, the effects pomegranate-derived (PENs) on nonalcoholic fatty liver disease (NAFLD) are still unknown. Here, PENs were successfully isolated characterized, found to be internalized by HepG2 cells. Interestingly, survived gastrointestinal environment ameliorated lipid accumulation as well...