A5032022895- Microbial Metabolic Engineering and Bioproduction
- Metabolism and Genetic Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Metabolomics and Mass Spectrometry Studies
- Enzyme Catalysis and Immobilization
- Biochemical and Molecular Research
- Proteins in Food Systems
- Gene Regulatory Network Analysis
- Meat and Animal Product Quality
- Biofuel production and bioconversion
- Insect Resistance and Genetics
- Sirtuins and Resveratrol in Medicine
- Mitochondrial Function and Pathology
- Microencapsulation and Drying Processes
- Algal biology and biofuel production
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- thermodynamics and calorimetric analyses
- Virus-based gene therapy research
- Polyamine Metabolism and Applications
- Neurological diseases and metabolism
Repsol (Spain)
2013-2023
Universidad de Murcia
2008-2018
Universidade Nova de Lisboa
2008-2011
Instituto de Biologia Experimental e Tecnológica
2008-2010
Universidad de Huelva
2004
University of Guelph
1985-1989
University of Alberta
1984-1985
Abstract Background Acetate metabolism in Escherichia coli plays an important role the control of central and bioprocess performance. The main problems related to use E. as cellular factory are i) deficient utilization carbon source due excretion acetate during aerobic growth, ii) inhibition growth protein production by iii) need for cofactor recycling (namely redox coenzymes free CoASH) sustain balanced homeostasis. Results This work analyzes effect mutations excretion/assimilation...
The cell density effect (i.e., the drop in specific productivity baculovirus-insect cells expression system when are infected at high densities) has been extensively described literature. In this article, a model for central metabolism of serum-free suspension cultures Spodoptera frugiperda Sf9 is proposed and used to investigate metabolic basis phenomenon. main pathways (glycolysis, pentose phosphate pathway, tricarboxylic acids cycle, glutaminolysis, amino metabolism), cellular growth...
Summary Lysine acetylation is a well‐established post‐translational modification widely conserved and distributed in bacteria. Although multiple regulatory roles have been proved, little known about its regulation. Here, we present evidence that the transcription of Gcn5‐like acetyltransferase YfiQ Escherichia coli (proposed name: PatZ) regulated by cAMP‐CRP implications on acetate metabolism The scavenging acetyl‐CoA synthetase (Acs) at transcriptional levels. Post‐translational regulation...
ABSTRACT Protein‐polysaccharide‐calcium gels were prepared using combinations of crude myofibrillar protein, whey protein concentrate, sodium alginate, methylcellulose, low methoxyl pectin, carrageenan and CaCl. The nature the interactions taking place in effect freezing or heating on viscosities water losses investigated. In most cases stability was increased by lowered heating. Myofibrillar with added concentrate formed stronger polysaccharide gums than did extract alone. With exception...
Abstract Background Stoichiometric models constitute the basic framework for fluxome quantification in realm of metabolic engineering. A recurrent bottleneck, however, is establishment consistent stoichiometric synthesis recombinant proteins or viruses. Although optimization algorithms silico redesign have been developed context genome-scale small molecule production, still rudimentary knowledge how different cellular levels are regulated and phenotypically expressed prevents their full...
Baculoviruses are insect viruses extensively exploited as eukaryotic protein expression vectors. Molecular biology studies have provided exciting discoveries on virus-host interactions, but the application of omic high-throughput techniques baculovirus-insect cell system has been hampered by lack host genome sequencing. While a broader, systems-level analysis biological responses to infection is urgently needed, recent advances proteomic yielded new insights impact cell. These works reviewed...
Different operating conditions of wet air oxidation and catalytic have been studied for the treatment highly concentrated methyldiethanolamine wastewater streams from amine units acid gas recovery in petrol refineries. These occasionally generate high content that require special actions to avoid undesirable impacts on downstream biological process petrochemical plant due its inhibition effect. The achieved remarkable removals methyldiethanolamine, sulfides, chemical oxygen demand total...
The halophilic bacterium Chromohalobacter salexigens has been proposed as promising cell factory for the production of compatible solutes ectoine and hydroxyectoine. This evolved metabolic adaptations to efficiently grow under high salt concentrations by accumulating ectoines solutes. However, overflow, which is a major drawback efficient conversion biological feedstocks, occurs result unbalances during growth production. Optimal conditioned interplay carbon nitrogen metabolisms. In this...
The halophilic bacterium Chromohalobacter salexigens is a natural producer of ectoines, compatible solutes with current and potential biotechnological applications. As production ectoines an osmoregulated process that draws away TCA intermediates, bacterial metabolism needs to be adapted cope salinity changes. To explore use C. as cell factory for ectoine(s) production, comprehensive knowledge at the systems level its essential. For this purpose, construction robust high-quality genome-based...
Abstract The increasing commercial demand for L-carnitine has led to a multiplication of efforts improve its production with bacteria. use different cell environments, such as growing, resting, permeabilized, dried, osmotically stressed, freely suspended and immobilized cells, maintain enzymes sufficiently active is discussed in the text. states enterobacteria, Escherichia coli Proteus sp ., which can be used produce from crotonobetaine or D-carnitine substrate, are analyzed. Moreover,...
Disruption of brain energy metabolism is the hallmark cerebral ischemia, a major cause death worldwide. Astrocytes play key role in regulation and their vulnerability to ischemia has been described. Aiming quantify effects an ischemic insult astrocytic metabolism, primary cultures astrocytes were subjected 5 h oxygen glucose deprivation bioreactor. Flux distributions, before after estimated by metabolic flux analysis using isotopic information consumption/secretion rates relevant...
The aim was to understand how interaction of the central carbon and secondary carnitine metabolisms is affected under salt stress its effect on production L-carnitine by Escherichia coli. biotransformation crotonobetaine into resting cells E. coli O44 K74 improved stress, a yield nearly twofold that for control being obtained with 0.5 M NaCl. Crotonobetaine formed acted as an osmoprotectant during cell growth in presence enzyme activities involved process (crotonobetaine hydration reaction...