A5056771762- Retinal Development and Disorders
- Optical Coherence Tomography Applications
- Corneal Surgery and Treatments
- Advanced Fluorescence Microscopy Techniques
- Corneal surgery and disorders
- Retinal Diseases and Treatments
- Barrier Structure and Function Studies
- Neurogenesis and neuroplasticity mechanisms
- Glaucoma and retinal disorders
- Digital Holography and Microscopy
- Ocular Surface and Contact Lens
- Stress Responses and Cortisol
- Cell Image Analysis Techniques
- Immunotherapy and Immune Responses
- Zebrafish Biomedical Research Applications
- Cell Adhesion Molecules Research
- Xenotransplantation and immune response
- Connexins and lens biology
- Adipose Tissue and Metabolism
- Ophthalmology and Visual Impairment Studies
- Advanced Proteomics Techniques and Applications
- Drug-Induced Ocular Toxicity
- Characterization and Applications of Magnetic Nanoparticles
- Photoreceptor and optogenetics research
- Sulfur Compounds in Biology
University Hospital Carl Gustav Carus
2013-2024
Technische Universität Dresden
2010-2024
Klinik und Poliklinik für Augenheilkunde am Universitätsklinikum Carl Gustav Carus
2023
German Center for Neurodegenerative Diseases
2021
Goethe University Frankfurt
2021
Tunneling nanotubes (TNTs) may offer a very specific and effective way of intercellular communication. Here we investigated TNTs in the human retinal pigment epithelial (RPE) cell line ARPE-19. Morphology was examined by immunostaining scanning electron microscopy. To determine function between cells, studied TNT-dependent communication at different levels including electrical calcium signalling, small molecular diffusion as well mitochondrial re-localization. Further, organelles transfer...
The neural crest-derived adrenal medulla is closely related to the sympathetic nervous system; however, unlike tissue, it characterized by high plasticity which suggests involvement of stem cells. Here, we show that a defined pool glia-like nestin-expressing progenitor cells in adult contributes this plasticity. These have features adrenomedullary sustentacular cells, are multipotent, and able differentiate into chromaffin neurons. central body's response stress making its proper adaptation...
Cell transplantation and stem cell therapy are promising approaches for regenerative medicine of interest to researchers clinicians worldwide. However, currently, no imaging technique that allows three-dimensional in vivo inspection therapeutically administered cells host tissues is available. Therefore, we investigate magnetomotive optical coherence tomography (MM-OCT) labeled with magnetic particles as a potential noninvasive tracking method. We develop mesenchymal future monitoring. Cells...
Perivascular cells of microvascular niches are the prime candidates for being a reservoire mesenchymal stem cell (MSC)-like in many tissues and organs that could serve as potential source target novel cell-based therapeutic approaches. In present study, by utilising typical markers pericytes (neuronal-glial antigen 2, NG2, chondroitin sulphate proteoglycan) those MSCs (CD146 CD105) primitive pluripotent (sex-determining region Y-box Sox2), phenotypic traits distribution murine rat retinal...
In this paper, we present a reliable and robust method for magnetomotive optical coherence tomography (MM-OCT) imaging of single cells labeled with iron oxide particles. This employs modulated longitudinal transverse magnetic fields to evoke alignment rotation anisotropic structures in the sample volume. Experimental evidence suggests that particles assemble themselves elongated chains when exposed permanent field. Magnetomotion intracellular space was detected visualized by means 3D OCT as...
Abstract Objective Investigating retinal thickness may complement existing biological markers for dementia and other neurodegenerative diseases. Although thinning is predictive cognitive decline, it remains to be investigated if how this feature aligns with neurodegeneration elsewhere in the brain, specifically early disease stages. Methods Using optical coherence tomography magnetic resonance imaging, we examined as well hippocampal structure patients amnestic mild impairment healthy...
Spectral domain dual-band optical coherence tomography for simultaneous imaging of rodent retina in the 0.8 μm and 1.3 wavelength region non-invasive monitoring posterior eye microstructure field retinal degeneration research is demonstrated. The system illuminated by a supercontinuum laser source allows three-dimensional with high axial resolution better than 3.8 5.3 tissue at 800 nm 1250 nm, respectively, precise thickness measurements. A fan-shaped scanning pattern pivot point close to...
Recent studies in animal models provided proof-of-principle evidence for cell transplantation as a potential future therapeutic approach retinal pathologies humans such Retinitis pigmentosa or age-related macular degeneration. In this case, donor cells are injected into the eye order to protect replace degenerating photoreceptors pigment epithelium. However, currently there is no three-dimensional imaging technique available that allows tracking of migration and integration host tissue under...
Purpose: The purpose of this study was to investigate corneal stiffening after epithelium-off accelerated cross-linking (CXL; 9 mW/cm²) in progressive keratoconus (KC) with different methods epithelial debridement. Methods: This a retrospective, interventional, and non-randomized study. In group 1, the epithelium removed using hockey knife (N = 45). 2 39) 3 22), thickness measured by optical coherence tomography (OCT) ablated excimer laser, but, 3, stromal ablation performed additionally...
Abstract Purpose To compare the parameters and indices of a novel swept‐source optical coherence tomography device (SS‐OCT, ANTERION) with those rotating Scheimpflug camera (RSC)‐based tomograph (Pentacam) in normal keratoconic (KC) eyes. Methods This prospective, monocentric, cross‐sectional study included individuals unoperated KC eyes, selecting one eye per subject. Ectasia‐specific analysed SS‐OCT were difference mean keratometry ( K ) inferior superior meridians, maximum value max ),...
For the study of disease mechanisms and development novel therapeutic strategies for retinal pathologies in human, rodent models play an important role. Nowadays, optical coherence tomography (OCT) allows three-dimensional investigation events over time. However, a detailed analysis how different degenerations are reflected OCT images is still lacking biomedical field. Therefore, we use to visualize degeneration specific mouse order progression <i>in vivo</i> improve image interpretation...
Abstract Background In vascular endothelial cells, cysteine metabolism by the cystathionine-γ lyase (CSE), generates hydrogen sulfide-related sulfane sulfur compounds (H2Sn), that exert their biological actions via S-sulfhydration of target proteins. This study set out to map “S-sulfhydrome” i.e. spectrum proteins targeted H2Sn in human cells. Methods LC-MS/MS was used identify S-sulfhydrated cysteines cell and β3 integrin intra-protein disulfide bond rearrangement. Functional studies...
The neural crest derived adrenal medulla is closely related to the sympathetic nervous system; however, unlike tissue, it characterized by high plasticity which suggests involvement of stem cells. In this study, using two transgenic mouse models, Nestin-GFP and inducible Nestin-CreER:Rosa26YFP, we define a pool multipotent glia-like nestin-GFP expressing progenitor cells in adult medulla, contribute its plasticity. plays central role response stress. However, adrenomedullary stress...
Zusammenfassung Hintergrund Die Chorioretinopathia centralis serosa (CCS) ist eine häufige Erkrankung, die durch Dekompensation des retinalen Pigmentepithels zum Verlust der Ionen-/Flüssigkeitshomöostase Retina und damit zur Ansammlung von Flüssigkeit mit Abhebung neurosensorischen und/oder führt. Wir untersuchten Auswirkung Eplerenon, einem Hemmer Mineralokortikoid-Rezeptors, auf chronisch rezidivierende CCS (cr-CCS). Methoden Es erfolgte retrospektive Auswertung Daten 17 Augen (12...