Michal P. Glogowski
A5013923590- Renal and related cancers
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- DNA Repair Mechanisms
- RNA modifications and cancer
- Cancer-related gene regulation
- Digestive system and related health
- Asymmetric Synthesis and Catalysis
- Radical Photochemical Reactions
- Coordination Chemistry and Organometallics
- Fluorine in Organic Chemistry
- Biochemical and Molecular Research
- Genomics and Chromatin Dynamics
- Endoplasmic Reticulum Stress and Disease
- Sulfur-Based Synthesis Techniques
- Catalytic C–H Functionalization Methods
South College
2021-2024
Providence College
2024
GlaxoSmithKline (United States)
2021
Microsatellite-unstable (MSI) cancers require WRN helicase to resolve replication stress due expanded DNA (TA)n dinucleotide repeats. is a promising synthetic lethal target for MSI tumors, and inhibitors are in development. In this study, we used CRISPR-Cas9 base editing map residues critical cells, validating the domain as primary drug target. Fragment-based screening led development of potent highly selective covalent inhibitors. These compounds selectively suppressed model growth vitro...
The discussion herein describes a metallaphotoredox reaction that allows for efficient exploration of benzyl structure–activity relationships in medicinal chemistry. use HTE (high-throughput experimentation) and ChemBeads rapid optimization. formation di(hetero)arylmethanes via cross-electrophile coupling between aryl bromides provides access to diverse chemical space. breadth the substrate scope will be discussed, along with utilization batch photochemistry preparation this...
<p>Supplementary Figure 1. Generating base editor-expressing MSI cell lines for screening. Supplementary 2. Quality control base-editor screens in lines. 3. Discovery and characterization of novel inhibitors WRN helicase. 4. Correlation inhibitor sensitivity with genetic molecular markers models. 5. Comparative analysis DNA damage MSS cells treated inactivation. 6: Effects GSK_WRN4 on colorectal cancer xenografts.</p>
<p>This table lists the results from Base editing screen</p>
<p>This file contains details of the BE sgRNA library</p>
<p>This file contain information on the Cell lines and organoids used in this study</p>
<div>Abstract<p>Microsatellite-unstable (MSI) cancers require WRN helicase to resolve replication stress due expanded DNA (TA)<sub>n</sub> dinucleotide repeats. is a promising synthetic lethal target for MSI tumors, and inhibitors are in development. In this study, we used CRISPR–Cas9 base editing map residues critical cells, validating the domain as primary drug target. Fragment-based screening led development of potent highly selective covalent inhibitors. These...
<p>Supplementary Figure 1. Generating base editor-expressing MSI cell lines for screening. Supplementary 2. Quality control base-editor screens in lines. 3. Discovery and characterization of novel inhibitors WRN helicase. 4. Correlation inhibitor sensitivity with genetic molecular markers models. 5. Comparative analysis DNA damage MSS cells treated inactivation. 6: Effects GSK_WRN4 on colorectal cancer xenografts.</p>
<p>This file contain information on the Cell lines and organoids used in this study</p>
<p>This table lists the results from Base editing screen</p>
<p>This file contains details of the BE sgRNA library</p>
<div>Abstract<p>Microsatellite-unstable (MSI) cancers require WRN helicase to resolve replication stress due expanded DNA (TA)<sub>n</sub> dinucleotide repeats. is a promising synthetic lethal target for MSI tumors, and inhibitors are in development. In this study, we used CRISPR–Cas9 base editing map residues critical cells, validating the domain as primary drug target. Fragment-based screening led development of potent highly selective covalent inhibitors. These...
A two-step metal–halogen exchange and diastereoselective copper-mediated Michael addition onto a complex α,β-unsaturated system has been developed applied toward the synthesis of bisaryl Nrf2 activators. Optimization using (n-Bu)3MgLi allowed for preparation custom aryl-functionalized magnesiate reagents at noncryogenic temperatures. Following transmetalation, these were used in highly reactions.