A5091801810- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Heart Failure Treatment and Management
- COVID-19 Clinical Research Studies
- Nanopore and Nanochannel Transport Studies
- SARS-CoV-2 and COVID-19 Research
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Long-Term Effects of COVID-19
- Analytical Chemistry and Chromatography
- Computational Drug Discovery Methods
- Nicotinic Acetylcholine Receptors Study
- Prostate Cancer Treatment and Research
- Fuel Cells and Related Materials
- Ion-surface interactions and analysis
- Inflammasome and immune disorders
- Gout, Hyperuricemia, Uric Acid
- Acute Kidney Injury Research
- Diabetes and associated disorders
- Chronic Kidney Disease and Diabetes
- Mechanical Circulatory Support Devices
- Cardiovascular Function and Risk Factors
- Protease and Inhibitor Mechanisms
- Cancer Cells and Metastasis
- Cancer Diagnosis and Treatment
Hôpital Lariboisière
2014-2024
Inserm
2012-2024
Université Paris Cité
2009-2024
NeuroDiderot
2019-2024
Délégation Paris 7
2014-2024
Assistance Publique – Hôpitaux de Paris
2014-2024
Celescreen (France)
2020
Assistance Publique Hôpitaux de Marseille
2020
Université Paris Nanterre
2009-2020
Hôpital Fernand-Widal
2002-2020
There are still unmet needs in finding new technologies for biomedical diagnostic and industrial applications. A technology allowing the analysis of size sequence short peptide molecules only few molecular copies is challenging. The fast, low-cost label-free single-molecule nanopore could be an alternative addressing these critical issues. Here, we demonstrate that wild-type aerolysin enables size-discrimination several uniformly charged homopeptides, mixed solution, with a single amino acid...
Untreated gout is characterised by monosodium urate (MSU) crystal accumulation responsible for recurrent flares that are commonly separated asymptomatic phases. Both phases inflammatory conditions of variable intensity. Gout self-limited reactions involving multiple mediators. This study aimed to characterise the profiles at different
Cystatin C (CST3), a strong inhibitor of cysteine proteinases, is freely filtered by the kidney glomerulus and reabsorbed tubules, where it almost totally catabolized, with remainder then eliminated in urine. In tubular diseases, seems sensible to postulate that CST3 degradation would be reduced consequently an increase its urinary elimination observed.We report here development automatic quantitative assay measure concentrations urine using Behring N-Latex kit on BNII laser nephelometer. We...
Neuropathological conditions might affect adult granulogenesis in the human dentate gyrus. However, radial glial cells (RGCs) have not been well characterized during development and aging. We previously described progenitor neuronal layer establishment hippocampal pyramidal gyrus from embryonic life until mid-gestation. Here, we describe RGC subtypes hippocampus 13 gestational weeks (GW) to mid-gestation characterize their evolution dynamics of neurogenesis adulthood normal Alzheimer's...
Increases in plasma B-type natriuretic peptide (BNP) concentrations those with acutely decompensated heart failure (ADHF) has been mainly attributed to an increase NPPB gene transcription. Recently, proBNP glycosylation emerged as a potential regulatory mechanism the production of amino-terminal (NT)-proBNP and BNP. The aim present study was investigate glycosylation, corin furin activities ADHF patients.Plasma levels proBNP, NT-proBNP, BNP, well concentration activity were measured large...
Background —Identification of factors regulating myocardial structure and function is important to understand the pathogenesis heart disease. Because little known about molecular mechanism cardiac functions triggered by serotonin, link between downstream signaling circuitry its receptors physiology widespread interest. None serotonin receptor (5-HT 1A , 5-HT 1B or 2C ) disruptions in mice have resulted cardiovascular defects. In this study, we examined 2B receptor–mutant assess putative role...
This study sought to hypothesize that elevated B-type natriuretic peptide (BNP) could act as an endogenous neprilysin inhibitor. A hallmark of acute decompensated heart failure (ADHF) is the overproduction peptides (NPs) by stretched cardiomyocytes. Various strategies have been developed potentiate beneficial effect NPs, including recent use angiotensin receptor inhibitors. Contrary rodents, human BNP poorly sensitive degradation while retaining affinity neprilysin. We enrolled 638 patients...
Early in the COVID-19 pandemic, type 2 diabetes (T2D) was marked as a risk factor for severe disease and mortality. Inflammation is central to aetiology of both conditions where variations immune responses can mitigate or aggravate course. Identifying at-risk groups based on immunoinflammatory signatures valuable directing personalised care developing potential targets precision therapy. This observational study characterised immunophenotypic variation associated with severity T2D....
Taking advantage of three cellular systems, we established that 5-HT<sub>2B</sub> receptors are coupled with NO signaling pathways. In the 1C11 serotonergic cell line and<i>Mastomys natalensis</i> carcinoid cells, which naturally express receptor, as well in transfected LMTK<sup>−</sup> fibroblasts, stimulation 5-HT<sub>2B</sub>receptor triggers intracellular cGMP production through dual activation constitutive nitric-oxide synthase (cNOS) and inducible NOS (iNOS). The group I PDZ motif at C...
Microenvironmental conditions in normal or tumour tissues and cell lines may interfere on further biological analysis. To evaluate transcript variations carefully, it is common to use stable housekeeping genes (HKG) normalise quantitative microarrays real-time polymerase chain reaction results. However, recent studies argue that HKG fluctuate according treatments. So, as an example of variation under array are the cancer field, we whether hypoxia could have impact expression. Expression 10...
TMEM127 is a novel pheochromocytoma (PCC) susceptibility gene.Our aim was to clearly determine the indications for genetic testing in patients with PCC and/or paraganglioma (PGL).Germline DNA from 642 unrelated who did not carry mutations major genes analyzed. Five hundred fifty-nine were affected by PCC, 72 PGL (22 head and neck 50 thoracic or abdominal location), 11 both PGL. Analysis of gene performed direct sequencing quantitative multiplex PCR short fluorescent fragments.In our cohort...
Background:Concentrations of the anti-angiogenic factor soluble fms-like tyrosine kinase-1 (sFlt-1) are altered in peripartum cardiomyopathy (PPCM). In this study we investigated changes angiogenesis balance PPCM.
Mandibuloacral dysplasia syndromes are mainly due to recessive LMNA or ZMPSTE24 mutations, with cardinal nuclear morphological abnormalities and dysfunction. We report five homozygous null mutations in MTX2, encoding Metaxin-2 (MTX2), an outer mitochondrial membrane protein, patients presenting a severe laminopathy-like mandibuloacral characterized by growth retardation, bone resorption, arterial calcification, renal glomerulosclerosis hypertension. Loss of MTX2 patients' primary fibroblasts...
Inherited prion diseases are characterized by mutations in the PRNP gene encoding protein (PrP). As other sporadic or infectious disease forms, they almost all accumulation brain of an abnormal misfolded form patient's PrP. Brain extracts can often transmit once inoculated a recipient animal. with Creutzfeldt-Jakob (CJD) phenotype autosomal although cases have been reported. We report three novel unrelated patients clinical and histopathologic features CJD. The were missense: c635G>A...
The study of signaling cascades and functional interactions between 5-hydroxytryptamine (5-HT) receptor pathways with heterogenous brain cell populations remains an arduous task. We took advantage a serotonergic line to elucidate cross-talks 5-HT receptors demonstrate the involvement two 5-HT2 subtypes in regulation 5-HT1B/1D function. inducible 1C11 has unique property acquiring within 4 days complete phenotype (1C11* cells), including three receptors. 5-HT2B are expressed since day 2...
The mechanisms by which weight loss decreases serum uric acid (SUA) levels are poorly known. We aimed to investigate the role played xanthine oxidase (XOD), metabolic status, and low-grade inflammation in decreased SUA induced obese patients.Data were from a series of consecutive patients with severe obesity involved bariatric surgery program. Measurements body composition biologic samples obtained before 6 months after surgery.Among 154 (mean ± SD age 41.0 12.3 years, mass index [BMI] 47.8...
One of the most important health challenges is early and ongoing detection disease for prevention, as well personalized treatment management. Development new sensitive analytical point-of-care tests are, therefore, necessary direct biomarker from biofluids critical tools to address healthcare needs an aging global population. Coagulation disorders associated with stroke, heart attack, or cancer are defined by increased level fibrinopeptide A (FPA) biomarker, among others. This exists in more...