A5064849293- Retinal Development and Disorders
- Glaucoma and retinal disorders
- Cell death mechanisms and regulation
- Retinopathy of Prematurity Studies
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Genetic Associations and Epidemiology
- Pharmacological Effects and Assays
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Retinal and Optic Conditions
- Zebrafish Biomedical Research Applications
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Neurobiology and Insect Physiology Research
- T-cell and B-cell Immunology
- Alzheimer's disease research and treatments
- Advanced Fluorescence Microscopy Techniques
- Corneal surgery and disorders
- Receptor Mechanisms and Signaling
- S100 Proteins and Annexins
- Melanoma and MAPK Pathways
- interferon and immune responses
- Traumatic Ocular and Foreign Body Injuries
Harvard University
2021-2025
Boston Children's Hospital
2021-2025
University of Wisconsin–Madison
2016-2023
Siena College
2015
New York State Department of Health
2013
Wadsworth Center
2013
Historically, association of disease with the major histocompatibility complex (HLA) genes has been tested HLA alleles that encode antigen-binding affinity. The Parkinson (PD), however, was discovered noncoding SNPs in a genome-wide study (GWAS). We show here several HLA-region have since associated PD form two blocks tagged by rs3129882 (p = 9 × 10−11) and rs9268515 and/or rs2395163 3 10−11). investigated whether these SNP-associations were driven HLA-alleles at adjacent loci. imputed class...
Abstract The brain controls nearly all bodily functions via spinal projecting neurons (SPNs) that carry command signals from the to cord. However, a comprehensive molecular characterization of brain-wide SPNs is still lacking. Here we transcriptionally profiled total 65,002 SPNs, identified 76 region-specific SPN types, and mapped these types into companion atlas whole mouse 1 . This taxonomy reveals three-component organization SPNs: (1) molecularly homogeneous excitatory cortex, red...
Abstract Axonal degeneration of retinal ganglion cells (RGCs) causes blindness in glaucoma. Currently, there are no therapies that target axons to prevent them from degenerating. Activation the BAX protein has been shown be determining step intrinsic apoptotic pathway RGCs die A putative role for axonal is less well elucidated. BCLX L (BCL2L1) primary antagonist RGCs. We developed a mCherry-BCLX fusion protein, which prevented recruitment and activation mitochondria tissue culture exposed...
Optineurin (OPTN) is a crucial component of the homeostatic pathway, playing pivotal role in regulating number essential signaling pathways including NF-kB, interferon, autophagy, and vesicular trafficking. The dysfunction OPTN has been implicated pathogenesis several diseases, such as primary open angle glaucoma (POAG), amyotrophic lateral sclerosis (ALS), frontotemporal lobar dementia. Interestingly, mutations are gain-of-function pathology loss-of-function ALS. However, remains unclear....
Pro-apoptotic BAX is a central mediator of retinal ganglion cell (RGC) death after optic nerve damage. activation occurs in two stages including translocation latent to the mitochondrial outer membrane (MOM) and then permeabilization MOM facilitate release apoptotic signaling molecules. As critical component RGC death, an attractive target for neuroprotective therapies understanding kinetics mechanisms controlling this process RGCs potentially valuable informing development strategy.
The α 2A ‐adrenergic receptor (α AR) is a member of the G‐protein coupled (GPCR) superfamily that responds to catecholamines such as epinephrine. In contrast β‐adrenergic receptors, 2 receptors act decrease blood pressure and control pain perception during “flight or fight” response. GPCR kinases (GRKs) are responsible for deactivating these by phosphorylating their third intracellular loop carboxyl terminal tail peptide. phosphate group attracts proteins known arrestins terminate signal....
Background Pro-apoptotic BAX is a central mediator of retinal ganglion cell (RGC) death after optic nerve damage. activation occurs in two stages including translocation latent to the mitochondrial outer membrane (MOM) and then permeabilization MOM facilitate release apoptotic signaling molecules. As critical component RGC death, an attractive target for neuroprotective therapies understanding kinetics mechanisms controlling this process RGCs potentially valuable informing development...
SUMMARY Neurodegenerative diseases are characterized by neuronal death and regenerative failure. However, gene regulatory programs governing how initial injuries lead to remain poorly understood. In adult mice, optic nerve crush (ONC) injury, which severs all axons of retinal ganglion cells (RGCs), results in massive axotomized RGCs failure survivors. We performed an vivo CRISPR/Cas9-based genome-wide screen 1893 transcription factors (TFs) seek repressors RGC survival axon regeneration...