A5071350063- Microtubule and mitosis dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Reproductive Biology and Fertility
- Advanced MEMS and NEMS Technologies
- Spaceflight effects on biology
- Plant Molecular Biology Research
- Acoustic Wave Resonator Technologies
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Protist diversity and phylogeny
- Mechanical and Optical Resonators
- Biocrusts and Microbial Ecology
- Hippo pathway signaling and YAP/TAZ
- Multi-Criteria Decision Making
- Pancreatic function and diabetes
- Nonlinear Dynamics and Pattern Formation
- Scheduling and Timetabling Solutions
- Carbohydrate Chemistry and Synthesis
- Fungal and yeast genetics research
- Genetic and Kidney Cyst Diseases
- Plant nutrient uptake and metabolism
Université de Montpellier
2010-2025
Centre National de la Recherche Scientifique
2011-2025
Institut Jacques Monod
2015-2025
Université Paris Cité
2014-2025
La Ligue Contre le Cancer
2022-2024
Centre de Recherche en Biologie cellulaire de Montpellier
2010-2024
National Oceanography Centre
2022
Délégation Paris 7
2014-2017
Sorbonne Paris Cité
2016-2017
Université de Montréal
2011-2014
Posttranslational glutamylation of tubulin is present on selected subsets microtubules in cells. Although the modification expected to contribute spatial and temporal organization cytoskeleton, hardly anything known about its functional relevance. Here we demonstrate that glutamylation, particular generation long glutamate side chains, promotes severing microtubules. In human cells, chains induces spastin-dependent microtubule disassembly and, consistently, only modified by are efficiently...
Polyglutamylation is a post-translational modification that generates lateral acidic side chains on proteins by sequential addition of glutamate amino acids. This was first discovered tubulins, and it important for several microtubule functions. Besides only the nucleosome assembly NAP1 NAP2 have been shown to be polyglutamylated. Here, using proteomic approach, we identify large number putative substrates polyglutamylation in HeLa cells. By analyzing selection these substrates, show them...
In most species, oocytes lack centrosomes. Accurate meiotic spindle assembly and chromosome segregation - essential to prevent miscarriage or developmental defects thus occur through atypical mechanisms that are not well characterized. Using quantitative in vitro vivo functional assays the C. elegans oocyte, we provide novel evidence kinesin-13 KLP-7 promotes destabilization of whole cellular microtubule network. By counteracting ectopic disorganization network, this function is strictly...
This paper presents fast switching RF microelectromechanical systems (MEMS) capacitors with measured times between 150-400 ns. By introducing bent sides on a planar microbeam, it is shown experimentally that the resonance frequency of aluminum bridges increased by factor 25 compared to standard MEMS components. In addition, this original shape can be implemented easily in post-processing complementary metal-oxide-semiconductor circuits. Several designs are presented mechanical frequencies...
During cytokinesis, the cell undergoes a dramatic shape change as it divides into two daughter cells. Cell changes in cytokinesis are driven by cortical ring rich actin filaments and nonmuscle myosin II. The closes via actomyosin contraction coupled with depolymerization. Of interest, closure hence furrow ingression nonconcentric (asymmetric) within division plane across Metazoa. This nonconcentricity can occur persist even without preexisting asymmetric cues, such spindle placement or...
Animal cell cytokinesis, or the physical division of one into two, is thought to be driven by constriction an actomyosin contractile ring at plane. The mechanisms underlying type-specific differences in cytokinesis remain unknown. Germ cells are totipotent that pass genetic information next generation. Previously, using formin cyk-1 (ts) mutant Caenorhabditis elegans 4-cell embryos, we found P2 germ precursor protected from failure and can divide with greatly reduced F-actin levels Here,...
MAST-like, or Greatwall (Gwl), an atypical protein kinase related to the evolutionarily conserved MAST family, is crucial for cell cycle control during mitotic entry. Mechanistically, activated by Cyclin B-Cdk1 phosphorylation of a 550 amino acids-long insertion in its activation segment. Subsequently, Gwl phosphorylates Endosulfine and Arpp19 convert them into inhibitors PP2A-B55 phosphatase, thereby preventing early dephosphorylation M-phase targets B-Cdk1. Here, searching elusive Gwl-like...
The physical separation of a cell into two daughter cells during cytokinesis requires cell-intrinsic shape changes driven by contractile ring. However, in vivo, interact with their environment, which includes other cells. How occurs tissues is not well understood. Here, we studied an intact animal tissue biogenesis. We used high-resolution microscopy and quantitative analysis to study the three rounds division C. elegans vulval precursor (VPCs). VPCs are cut half longitudinally each...
During cell division, chromosome segregation is orchestrated by a microtubule-based spindle. Interaction between spindle microtubules and kinetochores central to the bi-orientation of chromosomes. Initially dynamic allow assembly kinetochore attachments, which essential for alignment, are eventually stabilized efficient sister chromatids homologous chromosomes during mitosis meiosis I, respectively. Therefore, precise control microtubule dynamics utmost importance meiosis. Here, we study...
Abstract Entry into mitosis has been classically attributed to the activation of a cyclin B/Cdk1 amplification loop via partial pool this kinase becoming active at end G2 phase. However, how initial is activated still unknown. Here we discovered new role recently identified PP2A-B55 inhibitor FAM122A in triggering mitotic entry. Accordingly, depletion orthologue C. elegans prevents entry germline stem cells. Moreover, data from Xenopus egg extracts strongly suggest that FAM122A-dependent...
The microtubule-associated protein TOGp, which belongs to a widely distributed family from yeasts humans, is highly expressed in human tumors and brain tissue. From purified components we have determined the effect of TOGp on thermally induced tubulin association vitro presence 1 mm GTP 3.4 m glycerol. Physicochemical parameters describing mechanism polymerization were deduced kinetic curves by application classical theoretical models assembly. We calculated time range characteristic...
Microtubules (MTs) are cytoskeletal polymers that participate in diverse cellular functions, including cell division, intracellular trafficking, and templating of cilia flagella. MTs undergo dynamic instability, alternating between growth shortening via catastrophe rescue events. The rates frequencies MT parameters appear to be characteristic for a given type. We recently reported all vary throughout differentiation smooth muscle type intact Caenorhabditis elegans. Here we describe local...
In most species, oocytes lack centrosomes. Accurate meiotic spindle assembly and chromosome segregation - essential to prevent miscarriage or developmental defects thus occur through atypical mechanisms that are not well characterized. Using quantitative in vitro vivo functional assays the C. elegans oocyte, we provide novel evidence kinesin-13 KLP-7 promotes destabilization of whole cellular microtubule network. By counteracting ectopic disorganization network, this function is strictly...