A5027561338- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Renal and related cancers
- Ovarian function and disorders
- Tissue Engineering and Regenerative Medicine
- Reproductive Health and Technologies
- Seed Germination and Physiology
- Pluripotent Stem Cells Research
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Xenotransplantation and immune response
- Cancer Risks and Factors
- Birth, Development, and Health
- Urological Disorders and Treatments
- Trace Elements in Health
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cell death mechanisms and regulation
- Lipid metabolism and biosynthesis
- Prenatal Screening and Diagnostics
- Selenium in Biological Systems
- Epigenetics and DNA Methylation
- Phagocytosis and Immune Regulation
- Prenatal Substance Exposure Effects
University of Cologne
2015-2024
University Hospital Cologne
2011-2021
Klinik und Poliklinik für Psychosomatik und Psychotherapie
2020
Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe
2003-2016
Klinik für Frauenheilkunde
2003-2016
Instituto Nacional de las Mujeres
2013
Universität Ulm
2008-2012
University Hospital Ulm
2008-2012
UCLouvain
2011
Universidad de La Frontera
2011
BACKGROUND: In contrast to the technique of conventional freezing, vitrification spermatozoa requires high cooling rates (720 000°K/min), which could be damaging for spermatozoa. The aim our study was compare slowly frozen and vitrified in terms their post‐thaw DNA integrity motility. METHODS: Semen samples were prepared according routine swim‐up divided into aliquots comparison fresh, conventionally from same ejaculate presence or absence cryoprotectants. Spermatozoa motility determined....
This study investigates the ability of sucrose to protect spermatozoa against mitochondrial damage, artificial cryoinduction capacitation, and acrosome reaction. Spermatozoa were isolated using swim-up procedure performed three different media: (a) human tubal fluid (HTF, control) medium; (b) HTF with 1% serum albumin (HSA); (c) HSA 0.25 M sucrose. From each group, 30 mul suspensions cells dropped directly into liquid nitrogen stored for at least 24 h. Cells thawed by quickly submerging...
Cryopreservation as a process can be divided into two methods: conventional freezing and vitrification. The high effectiveness of vitrification in comparison with for human oocytes embryos is shown, whereas data on ovarian tissue are limited. aim this study was to compare the safety tissue. Ovarian fragments from 15 patients were transported laboratory within 22–25 h special, isolated transport box that maintain stable temperature between 5 8 °C 36 h. Small pieces (0.3–1×1–1.5×0.7–1 mm)...
ABSTRACT: The aim of this study was to develop and test the standardized aseptic technology permeable cryoprotectant‐free vitrification human spermatozoa in capillaries (for intracytoplasmic sperm injection [ICSI] or vitro fertilization [IVF]). To effect on basic parameters, each 68 swim‐up–prepared ejaculates from oligo‐astheno‐terato‐zoospermic patients were aliquoted distributed into 3 groups: 1) nontreated control, 2) 10 μL cryopreserved by slow conventional freezing with...
The aim of this study was to compare the viability human pronuclear oocytes subjected vitrification using cooling by direct submerging open-pulled straws in liquid nitrogen versus located inside a closed 0.5 ml straw (aseptic system).Two- and three-pronuclei stage (n=114) were cryopreserved super-open-pulled 20% ethylene glycol +20% dimethylsulphoxide (DMSO) + osmotic active neutral non-permeable cryoprotectants with four-step exposure 20, 33, 50 100% solution for 2, 1 min, 30-50 s,...
The ability of sucrose to protect spermatozoa against mitochondrial damage, artificial acrosome reaction and DNA fragmentation during ultra-rapid cryopreservation in canine sperm was investigated. Swim-up selected second-fraction semen were vitrified with different concentrations (0.1, 0.25 0.4 m) proportion 1 : v/v HTF–BSA 1%. From each group, 30-μl suspensions cells dropped directly into liquid nitrogen stored for at least 24 h. Cells thawed by submerging the spheres HTF 1% BSA 37 °C....
Herein, we report the birth of two healthy babies to a woman following intracytoplasmic sperm injection (ICSI) using motile spermatozoa vitrified without permeable cryoprotectants. Spermatozoa (in case oligoasthenoteratozoospermia) were cooled in cut standard straws human tubal fluid supplemented with 0.5% serum albumin and 0.25 M sucrose. Sperm motility, capacitation-like changes, acrosome reaction mitochondrial membrane potential (MMP) compared fresh spermatozoa. Eight mature (MII) oocytes...
The aims of this investigation were to test a novel technology comprising cryoprotectant-free vitrification the spermatozoa rainbow trout and study ability sucrose components seminal plasma protect these cells from cryo-injuries. Spermatozoa isolated vitrified using three different media: Group 1: standard buffer for fish spermatozoa, Cortland® medium (CM, control); 2: CM + 1% BSA 40% plasma; 3: 0.125 m sucrose. For cooling, 20-μl suspensions each group dropped directly into liquid nitrogen....
Abstract: We report the first case of a healthy baby born after intrauterine insemination with vitrified swim‐up spermatozoa from an oligoasthenozoospermic patient. A 39‐year‐old patient was subjected to her 35‐year‐old husband, diagnosed oligoasthenozoospermia. The 2 ejaculates were suspended in culture medium supplemented 1% human serum albumin and 0.25 M sucrose. Three hermetically packaged 100‐μL sperm portions (each containing 1.0 × 10 6 spermatozoa/mL) by direct plunging into liquid...
At present, there are three ways to determine effectively the quality of cryopreservation procedure using ovarian tissue before re-implantation treatment: evaluation follicles after post-thawing xenotransplantation SCID mouse, in-vitro culture in a large volume medium under constant agitation and on embryonic chorio-allantoic membrane within hen's eggs. The aim this study was compare two methods, vitro chorioallantoic (CAM) cryopreserved human medulla-contained medulla-free cortex. Ovarian...
Mitochondria-targeted antioxidants have great potential to counterbalance the generated reactive oxygen species (ROS) because they cross inner membrane of mitochondria. Still, their use was not reported in vitrified human spermatozoa. Our laboratory has successfully spermatozoa without permeable cryoprotectants, but subcellular-level evidence missing. Therefore, this study aimed improve vitrification using a mitochondria-targeted antioxidant (mitoquinone, MitoQ), reveal ultrastructural...