A5026009346- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial bioremediation and biosurfactants
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Transgenic Plants and Applications
- Protein purification and stability
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- Bacterial biofilms and quorum sensing
- Biotin and Related Studies
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Antibiotic Resistance in Bacteria
- DNA and Nucleic Acid Chemistry
- Animal Genetics and Reproduction
- Microbial Metabolic Engineering and Bioproduction
- Chromium effects and bioremediation
- Biochemical and Structural Characterization
- Advanced Fluorescence Microscopy Techniques
- RNA Interference and Gene Delivery
- Steroid Chemistry and Biochemistry
- Environmental remediation with nanomaterials
Bayer (Germany)
2012-2023
Bayer (United States)
2012
Yale University
1999
University of Illinois Urbana-Champaign
1990-1994
University of Illinois Chicago
1990-1994
Illinois College
1990-1994
Pseudomonas putida utilizes the catBC operon for growth on benzoate as a sole carbon source. This is positively regulated by CatR protein, which encoded from gene divergently oriented operon. The catR encodes 32.2-kilodalton polypeptide that binds to promoter region in presence or absence of inducer cis-cis-muconate, shown gel retardation studies. However, required transcriptional activation has been localized 385-base-pair fragment using broad-host-range promoter-probe vector pKT240. also...
Streptococcus pyogenes (Spy) Cas9 has potential as a component of gene therapeutics for incurable diseases. One its limitations is large size, which impedes formulation and delivery in therapeutic applications. Smaller Cas9s are an alternative, but lack robust activity or specificity frequently recognize longer PAMs. Here, we investigated four uncharacterized, smaller found three employing "GG" dinucleotide PAM similar to SpyCas9. Protein engineering generated synthetic RNA-guided nucleases...
The 3-chlorocatechol operon clcABD is central to the biodegradative pathway of 3-chlorobenzoate. clcR regulatory gene, which activates operon, was cloned from region immediately upstream and shown complement an insertion mutation for growth on ClcR activated clcA promoter, controls expression in trans by 14-fold vivo promoter probe assay Pseudomonas putida when cells were incubated with 15 mM 3-chlorobenzoic acid. Specific binding clcR-clcA intergenic observed a gel shift assay. Nucleotide...
ABSTRACT VanK is the fourth member of ubiquitous major facilitator superfamily transport proteins to be identified that, together with PcaK, BenK, and MucK, contributes aromatic catabolism in Acinetobacter sp. strain ADP1. PcaK have overlapping specificity for p -hydroxybenzoate and, most clearly, protocatechuate: inactivation both severely impairs growth protocatechuate, activity either protein alone can mask phenotype associated its homolog. Furthermore, vanK pcaK double-knockout mutants...
In therapeutic or diagnostic antibody discovery, affinity maturation is frequently required to optimize binding properties. some cases, achieving very high challenging using the display-based optimization technologies. Here we present an approach that begins with creation and clonal, quantitative analysis of soluble Fab libraries complete diversification in adjacent residue pairs encompassing every complementarity-determining region position. This was followed by alternative recombination...
Random Chimeragenesis on Transient Templates (RACHITT) has been used to create libraries averaging 12 () or even 19 (L. Encell, unpublished) crossovers per gene in a single round of family shuffling. RACHITT creates chimeric genes by aligning parental "donor" fragments full-length DNA template (). The heteroduplexed top strand are stabilized the single, long annealing step, taking advantage full length binding each fragment, rather than smaller overlaps, and carrying out reactions at...
Previous studies have shown that the clcABD operon is under transcriptional control of LysR-type activator ClcR. In this study, conditions leading to its aggregation were avoided and ClcR was purified confirmed by amino-terminal sequencing. Gel filtration indicated exists as a dimer in solution. The DNase I footprint determined. binding properties catechol regulator, CatR, compared.