A5033914169- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Lipid Membrane Structure and Behavior
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Inorganic and Organometallic Chemistry
- Amino Acid Enzymes and Metabolism
- Metal complexes synthesis and properties
- Plant Gene Expression Analysis
- RNA and protein synthesis mechanisms
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Organometallic Complex Synthesis and Catalysis
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Genetic Neurodegenerative Diseases
- Biochemical and Molecular Research
- RNA Interference and Gene Delivery
- Polyamine Metabolism and Applications
- Transgenic Plants and Applications
- ATP Synthase and ATPases Research
- Organometallic Compounds Synthesis and Characterization
- Galectins and Cancer Biology
- Protein Tyrosine Phosphatases
ProterixBio (United States)
2024-2025
University of Leeds
2016-2023
Aalborg University
2023
Universität Greifswald
2013-2022
Institute of Structural and Molecular Biology
2020
Universität Hamburg
2011
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrganometallic conformational equilibriums. X. Steric factors and their mechanistic implications in .pi.-allyl(amine)chloropalladium(II) complexesJ. W. Faller, M. E. Thomsen, J. MattinaCite this: Am. Chem. Soc. 1971, 93, 11, 2642–2653Publication Date (Print):June 1, 1971Publication History Published online1 May 2002Published inissue 1 June 1971https://pubs.acs.org/doi/10.1021/ja00740a011https://doi.org/10.1021/ja00740a011research-articleACS...
Abstract Flavonoids are a large group of plant secondary metabolites with variety biological properties and therefore interest to many scientists, as they can lead industrially interesting intermediates. The anaerobic gut bacterium Eubacterium ramulus catabolize flavonoids, but until now, the pathway has not been experimentally confirmed. In present work, chalcone isomerase (CHI) an enoate reductase (ERED) could be identified through whole genome sequencing gene motif search. These two...
Kinesin-1 transports numerous cellular cargoes along microtubules. The kinesin-1 light chain (KLC) mediates cargo binding and regulates motility. To investigate the molecular basis for recruitment activation by cargoes, we solved crystal structure of KLC2 tetratricopeptide repeat (TPR) domain bound to JIP3. This, combined with biophysical evolutionary analyses, reveals a site, located on KLC TPR1, which is conserved in homologs from sponges humans. In complex, JIP3 crosslinks two TPR domains...
Significance Autoimmune disease pathogenesis is driven by inflammation, induced partly IgG autoantibody-containing immune complexes binding to Fc gamma receptors (FcγRs). These are valid therapeutic targets in the treatment of autoimmunity. FcγRIIIa one a family highly homologous for antibodies; previous attempts at blockade have resulted off-target effects involving cells that express almost identical protein FcγRIIIb. Here we report identification functionally specific protein-based...
Membrane proteins are traditionally extracted and purified in detergent for biochemical structural characterisation. This process is often costly laborious, the stripping away of potentially stabilising lipids from membrane protein interest can have detrimental effects on integrity. Recently, styrene-maleic acid (SMA) co-polymers offered a solution to this problem by extracting directly their native membrane, while retaining naturally associated form stable SMA lipid particles (SMALPs)....
The importance of amine transaminases for producing optically pure chiral precursors pharmaceuticals and chemicals has substantially increased in recent years. X-ray crystal structure the (R)-selective transaminase from fungus Aspergillus fumigatus was solved by S-SAD phasing to 1.84 Å resolution. refined at 1.27 resolution provides detailed knowledge about molecular basis substrate recognition conversion facilitate protein-engineering approaches. protein forms a homodimer belongs fold class...
Chiral amines are important precursors for the pharmaceutical and fine-chemical industries. Because of this, demand enantiopure is currently increasing. Amine transaminases can produce a large spectrum chiral in (R)- or (S)-configuration, depending on their substrate scope stereo-preference, by converting prochiral ketone into amine while using alanine as donor producing pyruvate an α-keto acid product. In order to guide protein engineering improve specificity enantioselectivity, we carried...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrganometallic conformational equilibria. III. Epimerization mechanism of .pi.-allyl(amine)palladium(II) chloride complexesJohn W. Faller, M. J. Incorvia, and E. ThomsenCite this: Am. Chem. Soc. 1969, 91, 2, 518–519Publication Date (Print):January 1, 1969Publication History Published online1 May 2002Published inissue 1 January 1969https://pubs.acs.org/doi/10.1021/ja01030a064https://doi.org/10.1021/ja01030a064research-articleACS PublicationsRequest...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrganometallic conformational equilibria. VIII. Spin saturation labeling studies of the epimerization and isomerization mechanism 1,2,3-trihapto-(3-acetyl-2-methylallyl)[(S)-.alpha.-phenethylamine]-chloropalladium(II)John W. Faller M. E. ThomsenCite this: J. Am. Chem. Soc. 1969, 91, 24, 6871–6874Publication Date (Print):November 1, 1969Publication History Published online1 May 2002Published inissue 1 November...
Flavonoids represent a large class of secondary metabolites produced by plants. These polyphenolic compounds are well known for their antioxidative abilities, antimicrobial phytoalexins responsible flower pigmentation to attract pollinators and, in addition other properties, also specific bacterial regulators governing the expression Rhizobium genes involved root nodulation (Firmin et al., 1986). The chalcone isomerase (CHI) from Eubacterium ramulus catalyses first step flavanone-degradation...
Deleterious mutations in the X-linked Patched domain-containing 1 (PTCHD1) gene may account for up to 1% of autism cases. Despite this, PTCHD1 protein remains poorly understood. Structural similarities family proteins point a role sterol transport, but this hypothesis has not been verified experimentally. Additionally, suggested be involved Hedgehog signalling, thus far, experimental results have conflicting. To enable variety biochemical and structural experiments, we developed method...
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent events require additional protein machinery. No mechanism membrane arising from simply a binding to glycolipids has been described thus far. Herein, we report that biotinylated derived cholera toxin becomes fusogenic lectin upon cross-linking with streptavidin. This novel reengineered brings about hemifusion...
Abstract FAN1 is an endo- and exo-nuclease involved in DNA interstrand crosslink repair. Genome-wide association studies of people with Huntington’s disease revealed a strong between the R507H mutation early onset, however underlying mechanism(s) remains unclear. has previously been implicated modulating triplet repeat expansion PCNA dependent manner. To examine role on activation, we solved cryo-EM structures PCNA–FAN1–DNA complex. Our findings reveal that R507 residue directly interacts...
The (R)-selective amine transaminase from Aspergillus fumigatus was expressed in Escherichia coli and purified to homogeneity. Bright yellow crystals appeared while storing the concentrated solution refrigerator belonged space group C222(1). X-ray diffraction data were collected 1.27 Å resolution, as well an anomalous set 1.84 resolution that suitable for S-SAD phasing.
Abstract Human spermine oxidase (hSMOX) plays a central role in polyamine catabolism. Due to its association with several pathological processes, including inflammation and cancer, hSMOX has garnered interest as possible therapeutic target. Therefore, determination of the structure is an important step enable drug discovery validate Using insights from hydrogen/deuterium exchange mass spectrometry (HDX-MS), we engineered construct obtain first crystal bound known inhibitor MDL72527 at 2.4 Å...
Abstract Flavonoide stellen eine große Gruppe pflanzlicher Sekundärmetabolite dar und haben Vielzahl unterschiedlicher biologischer Eigenschaften. Aufgrund dessen stehen sie im Interesse vieler Wissenschaftler, da zu vielen industriell interessanten Verbindungen führen können. Das anaerobe Darmbakterium Eubacterium ramulus kann über einen bislang unbekannten Abbauweg diese verstoffwechseln. Über Vollgenom‐Sequenzierung wurden in dieser Arbeit die entscheidenden Enzyme, Chalconisomerase (CHI)...
Nucleoside transporters play critical biological roles in humans, and to understand the molecular mechanism of nucleoside transport requires high-resolution structural information. However, main bottleneck for analysis is production pure, stable high quality native protein crystallization trials. Here we report a novel membrane expression purification strategy, including construction high-yield vector, new fast protocol transporters. The advantages this strategy are improved time efficiency,...
Orthosteric inhibitors of the human heterodimeric DNA mismatch repair complex MutSbeta were identified by high-throughput screening. Following extensive hit confirmation to remove false positives, two series found give consistent activity free likely artefactual effects. Extensive profiling confirmed an ATP-competitive mode action, and X-ray crystallography showed occupying ATP-binding site MSH3.
The Haemophilus surface fibril (Hsf) is an unusually large trimeric autotransporter adhesin (TAA) expressed by the most virulent strains of H. influenzae . Hsf known to mediate adhesion between pathogen and host, allowing establishment potentially deadly diseases such as epiglottitis, meningitis pneumonia. While recent research has suggested that this TAA might adopt a novel `hairpin-like' architecture, characterization been limited in silico modelling electron micrographs, with no...
djbZ Zeitschrift des Deutschen Juristinnenbundes , Seite 164 - 168
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent events require additional protein machinery. No mechanism membrane arising from simply a binding to glycolipids has been described thus far. Herein we report that biotinylated derived cholera toxin, becomes fusogenic lectin upon crosslinking with streptavidin. This novel reengineered brings about hemifusion...