A5100617797- Ion Channels and Receptors
- Transgenic Plants and Applications
- Retinal and Macular Surgery
- Cardiac Valve Diseases and Treatments
- Intraocular Surgery and Lenses
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Cystic Fibrosis Research Advances
- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Calcium signaling and nucleotide metabolism
- Toxin Mechanisms and Immunotoxins
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Cancer, Hypoxia, and Metabolism
- Aortic Disease and Treatment Approaches
- Pancreatic function and diabetes
- Neonatal Respiratory Health Research
- Neuroscience of respiration and sleep
- Inflammasome and immune disorders
- Monoclonal and Polyclonal Antibodies Research
- Neuropeptides and Animal Physiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Infective Endocarditis Diagnosis and Management
- Phytochemicals and Antioxidant Activities
University of Florida
2009-2024
Catholic University of Korea
2006-2024
Seoul St. Mary's Hospital
2019-2024
Yonsei University
2014-2024
Chung-Ang University
2014-2024
Chung-Ang University Hospital
2024
Stanford University
2022-2023
Hanil General Hospital
2021-2023
Seoul National University
2016-2022
Korea Institute of Brain Science
2022
The effect of surfactin on the proliferation LoVo cells, a human colon carcinoma cell line, was examined. Surfactin strongly blocked cells by inducing pro-apoptotic activity and arresting cycle, according to several lines evidence DNA fragmentation, Annexin V staining, altered levels poly (ADP-ribose) polymerase, caspase-3, p21(WAF1/Cip1), p53, CDK2 cyclin E. anti-proliferative mediated inhibiting extracellular-related protein kinase phosphoinositide 3-kinase/Akt activation, as assessed...
In Petunia×hybrida cv 'Mitchell Diploid' (MD), floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is controlled spatially, developmentally, and daily at molecular, metabolic, biochemical levels. Multiple genes have been shown to encode proteins that either directly catalyse a reaction yielding FVBP compounds or are involved in metabolite flux prior the formation of compounds. It was hypothesized multiple transcription factors precise regulation all necessary genes, resulting...
Abstract Although autophagy is critical for pancreatic β-cell function, the role and mechanism of mitophagy in β-cells are unclear. We studied lysosomal Ca 2+ TFEB activation by mitochondrial or metabolic stress that TFEB-mediated function. Mitochondrial induced through release, increased cytosolic activation. Lysosomal replenishment ER - > lysosome refilling was essential mitophagy. β-cell-specific Tfeb knockout ( Δβ-cell ) abrogated high-fat diet (HFD)-induced mitophagy, accompanied ROS...
Store-operated Ca2+ channels (SOCs) mediate receptor-stimulated influx. Accumulating evidence indicates that members of the transient receptor potential (TRP) channel family are components SOCs in mammalian cells. Agonist stimulation activates and TRP directly by inducing translocation intracellular vesicles to plasma membrane (PM). The mechanism response store depletion agonist is not known. Here we use TRPC3 as a model show IP3 scaffold Homer 1 (H1) regulate rate retrieval from PM. In...
Induction of endoplasmic reticulum ( ER )‐to‐Golgi blockade or stress induces Golgi reassembly stacking protein GRASP )‐mediated, Golgi‐independent unconventional cell‐surface trafficking the folding‐deficient ΔF508 ‐cystic fibrosis transmembrane conductance regulator CFTR ). However, molecular mechanisms underlying this process remain elusive. Here, we show that phosphorylation‐dependent dissociation homotypic complexes and subsequent relocalization to play a critical role in secretion ....
The pancreatic duct expresses cystic fibrosis transmembrane conductance regulator (CFTR) and HCO3- secretory salvage mechanisms in the luminal membrane. Although CFTR plays a prominent role secretion, of is not known. In present work, we used molecular, biochemical, functional approaches to study regulatory interaction between mechanism Na+/H+ exchanger isoform 3 (NHE3) heterologous expression systems native duct. We found that regulates NHE3 activity by both acute chronic mechanisms. duct,...
TNF superfamily member 13 (TNFSF13) has been identified as a susceptibility gene for IgA nephropathy in recent genetic studies. However, the role of TNFSF13 progression remains unresolved. We evaluated two polymorphisms (rs11552708 and rs3803800) plasma levels 637 patients with nephropathy, determined risk ESRD according to theses variable. Neither examined associated clinical outcome nephropathy. high increased ESRD. To explore causal relationship underlying mechanism, we treated B cells...
This retrospective study was to compare the efficacy of intravitreal injection ranibizumab and aflibercept for patients with pachychoroid neovasculopathy. 54 eyes were initially treated 3 monthly loading injections or aflibercept. Treatment switching from aflibercept, photodynamic therapy done at months in case incomplete fluid absorption. At months, rate complete absorption significantly higher aflibercept-treated group than ranibizumab-treated (82.6% vs 51.6%, p = 0.018). The mean...
6-Hydroxydopamine (6-OHDA) is a neurotoxin and commonly used to generate experimental models of Parkinson's disease (PD). In this study, we investigated the signaling molecules involved in 6-OHDA-induced cell death using neuronal catecholaminergic line (SK-N-SH cells), protective effect fustin, flavonoid from Rhus verniciflua Stokes, on death. 6-OHDA significantly increased levels reactive oxygen species (ROS), intracellular Ca2+ ([Ca2+]i), p38 phosphorylation. addition, ROS increase by was...
In Petunia x hybrida cv. 'Mitchell Diploid' floral fragrance is comprised of 13 volatile benzenoids/phenylpropanoids derived from the aromatic amino acid phenylalanine. Several genes involved in direct synthesis individual benzenoid/phenylpropanoid (FVBP) compounds, i.e. at end pathway, have been isolated and characterized petunia through reverse genetic biochemical approaches. an effort to understand regulation 'upstream' components FVBP system, we cloned two CHORISMATE MUTASE (PhCM1 PhCM2)...
Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is a complex and coordinate cellular process executed by petal limb cells of Petunia×hybrida cv. 'Mitchell Diploid' (MD) plant. In MD flowers, the majority benzenoid compounds are derived from core phenylpropanoid pathway intermediate coenzyme A (CoA) dependent, β-oxidative scheme. Metabolic flux analysis, reverse genetics, biochemical characterizations key enzymes in this have supported putative concept. However, theoretical...
Granular corneal dystrophy type 2 (GCD2) is an autosomal dominant disease characterized by a progressive age-dependent extracellular accumulation of transforming growth factor β-induced protein (TGFBI). Corneal fibroblasts from GCD2 patients also have degenerative features, but the mechanism underlying this degeneration remains unknown. Here we observed that TGFBI was degraded autophagy, not ubiquitin/proteasome-dependent pathway. We found homozygous displayed greater number fragmented...
CFTR is a transmembrane protein that reaches the cell surface via conventional Golgi mediated secretion pathway. Interestingly, ER-to-Golgi blockade or ER stress induces alternative GRASP-mediated, Golgi-bypassing unconventional trafficking of wild-type and disease-causing ΔF508-CFTR, which has folding defects. Here, we show Sec16A, key regulator transport, plays critical role in exit cargos during secretion. In an initial gene silencing screen, Sec16A knockdown abolished ΔF508-CFTR induced...