A5006950375- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Plant Reproductive Biology
- Aluminum toxicity and tolerance in plants and animals
- Legume Nitrogen Fixing Symbiosis
- ATP Synthase and ATPases Research
- Plant Physiology and Cultivation Studies
- Plant Micronutrient Interactions and Effects
- Mycorrhizal Fungi and Plant Interactions
- Soil Carbon and Nitrogen Dynamics
- Protist diversity and phylogeny
- Plant Disease Resistance and Genetics
- Soil and Water Nutrient Dynamics
- Wastewater Treatment and Nitrogen Removal
- Phytoplasmas and Hemiptera pathogens
- Plant Virus Research Studies
- Bacterial Genetics and Biotechnology
- Plant responses to water stress
Centre National de la Recherche Scientifique
2013-2023
Institut de Biologie Moléculaire des Plantes
2013-2023
Université de Strasbourg
2012-2023
Ecologie fonctionnelle & biogéochimie des sols & des agro-systèmes
2007-2009
Institut de Recherche pour le Développement
2009
University of York
2006-2008
Institut National de la Recherche Agronomique
2002
Manganese toxicity is a major problem for plant growth in acidic soils, but cellular mechanisms that facilitate such conditions have not been clearly delineated. Established counter metal plants involve chelation and cytoplasmic export of the across plasma or vacuolar membranes out cell sequestered into large organelle, respectively. We report here expression Arabidopsis poplar MTP11 cation diffusion facilitators manganese-hypersensitive yeast mutant restores manganese tolerance to wild-type...
The Arabidopsis thaliana genome contains five genes that encode two pore K+ (TPK) channels. most abundantly expressed isoform of this family, TPK1, is at the tonoplast where it mediates -selective currents between cytoplasmic and vacuolar compartments. TPK1 open probability depends on both Ca2+ pH but not membrane voltage. channel shows intrinsic rectification can be blocked by Ba2+, tetraethylammonium, quinine. current was found in all shoot cell types hallmarks previously described K (VK)...
The Arabidopsis thaliana genome contains 20 cyclic nucleotide gated channel (CNGC) genes encoding putative non-selective ion channels. Classical and reverse genetic approaches have revealed that two members of this family (CNGC2 CNGC4) play a role in plant defence responses whereas CNGC1 CNGC10 may participate heavy metal cation transport. Yet, it remains to be resolved how the transport attributes CNGCs are integrated into their physiological function. In study, CNGC3 is characterized...
RNase P is an essential enzyme that cleaves the 5′ leader sequence of tRNA precursors. Ps were believed until now to occur universally as ribonucleoproteins in organisms performing activity. Here we find protein-only enzymes called PRORP (for proteinaceous P) support activity vivo both organelles and nucleus Arabidopsis . Beyond tRNA, proteins are involved maturation small nucleolar RNA (snoRNA) mRNA. Finally, ribonucleoprotein MRP not plants. Altogether, our results indicate have been...
Abstract Following the endosymbiotic acquisition of mitochondria by eukaryotic cells, most genes in this organelle were transferred to nucleus. To maintain mitochondrial biogenesis and function, nuclear genomes require regulated coordinated expression. In plant organelles, nuclear-encoded proteins targeted organelles control posttranscriptional posttranslational mechanisms. Pentatricopeptide repeat (PPR) are good candidates play such regulatory roles. Here, we identify PNM1 (for PPR protein...
PlantRNA database (http://plantrna.ibmp.cnrs.fr/) compiles transfer RNA (tRNA) gene sequences retrieved from fully annotated plant nuclear, plastidial and mitochondrial genomes. The set of tRNA has been manually curated for maximum quality confidence. novelty this resides in the inclusion biological information relevant to function all tRNAs entered library. This includes 5'- 3'-flanking sequences, A B box region transcription initiation poly(T) termination stretches, intron aminoacyl-tRNA...
RNase P is the essential activity removing 5'-leader sequences from transfer RNA precursors. was always associated with ribonucleoprotein complexes before discovery of protein-only enzymes called PRORPs (PROteinaceous P) in eukaryotes. Here we provide biophysical and functional data to understand mode action PRORP enzymes. Activity assays footprinting experiments show that anticodon domain dispensable, whereas individual residues D TψC loops are for function. proteins characterized solution...
The RNA exosome provides eukaryotic cells with an essential 3'-5' exoribonucleolytic activity, which processes or eliminates many classes of RNAs. Its nine-subunit core (Exo9) is structurally related to prokaryotic phosphorolytic exoribonucleases. Yet, yeast and animal Exo9s have lost the primordial capacity rely instead on associated hydrolytic ribonucleases for catalytic activity. Here, we demonstrate that Arabidopsis Exo9 has retained a distributive contributes rRNA maturation processes,...
All tRNA His possess an essential extra G –1 guanosine residue at their 5′ end. In eukaryotes after standard processing by RNase P, is added a guanylyl transferase. prokaryotes, genome-encoded and retained during maturation. plant mitochondria, although trnH genes we find here that both maturation pathways can be used. Indeed, with or without are found in mitochondrial fraction. Furthermore, recombinant Arabidopsis P cleave precursors positions +1 . The for recognition histidyl-tRNA...
• A different NO3− dependent pattern of uptake at low [NO3−] (0–0.1 mM) is shown in Pinus pinaster and the ectomycorrhizal fungus Rhizopogon roseolus. In symbiosis, fungal pre-eminent. Net rates were deduced plant from solution depletion measurements. fluxes estimated surface mycorrhizal nonmycorrhizal short roots, using selective microelectrodes. starved seedlings, maximum reached after 3 d incubation 0.05 mM NO3−. R. roseolus mycelia, did not change withdrawing for up to 7 d, or adding d....
ABSTRACT The impact of mineral N supply, N‐free or NO 3 ‐ with without NH 4 + , on the subsequent uptake by maritime pine seedlings associated ectomycorrhizal fungus Rhizopogon roseolus was studied using ion‐selective microelectrodes. net fluxes into N‐starved non‐mycorrhizal short roots (NMSRs) were low and measurable only over concentration range 0–70 µ M. simple kinetics observed in those may reflect dominant operation a high‐affinity transport system (HATS) which is constitutive....
The maturation of tRNA precursors involves the 5' cleavage leader sequences by an essential endonuclease called RNase P. Beyond ancestral ribonucleoprotein (RNP) P, a second type P PRORP (protein-only P) evolved in eukaryotes. current view on distribution cells is that multiple RNPs, PRORPs or combination both, perform specialised activities different compartments where gene expression occurs. Here, we identify single encoding green alga Chlamydomonas reinhardtii while no RNP found. We show...
Abstract In eukaryotes, general mRNA decay requires the decapping complex. The activity of this complex depends on its catalytic subunit, DECAPPING2 (DCP2), and interaction with enhancers, including main partner DECAPPING1 (DCP1). Here, we report that in Arabidopsis thaliana, DCP1 also interacts a NYN domain endoribonuclease, hence named DCP1-ASSOCIATED ENDORIBONUCLEASE 1 (DNE1). Interestingly, found DNE1 predominantly associated DCP1, but not DCP2, reciprocally, suggesting existence two...
Plant viruses cause massive crop yield loss worldwide. Most plant are RNA viruses, many of which contain a functional tRNA-like structure. RNase P has the enzymatic activity to catalyze 5' maturation precursor tRNAs. It is also able cleave structures. However, enzymes only accumulate in nucleus, mitochondria, and chloroplasts rather than cytosol where virus replication takes place. Here, we report biotechnology strategy based on re-localization protein-only (CytoRP) target structures thus...
The novel pentatricopeptide repeat protein PNM1 has recently been shown to be dual localized the nucleus and mitochondria. In it binds proteins involved in regulating gene expression, especially a TCP transcription factor. This class of was control expression nuclear genes encoding mitochondrial that contain cis-acting "site II" regulatory elements their promoter regions. analysis mutant plants showed some with site II have increased levels when is not present nucleus. suggests might act as...