A5046003977- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Phagocytosis and Immune Regulation
- Photosynthetic Processes and Mechanisms
- Bioinformatics and Genomic Networks
- Autophagy in Disease and Therapy
- Protein Structure and Dynamics
- Pluripotent Stem Cells Research
- Computational Drug Discovery Methods
- MicroRNA in disease regulation
- Melanoma and MAPK Pathways
- Telomeres, Telomerase, and Senescence
- Cell death mechanisms and regulation
University of Toronto
2019-2025
Hospital for Sick Children
2016-2025
Highly efficient generation of deletions, substitutions, and small insertions (up to ~ 150 bp) into the Caenorhabditis elegans genome by CRISPR/Cas9 has been facilitated use single-stranded oligonucleotide donors as repair templates. However, insertion larger sequences such fluorescent markers other functional domains remains challenging due uncertainty optimal performance between or double-stranded templates labor-intensive well inefficient protocols for their preparations. Here, we...
Apoptosis is a genetically programmed cell death process with profound roles in development and disease. MicroRNAs modulate the expression of many proteins are often deregulated human diseases, such as cancer. C. elegans germ cells undergo apoptosis response to genotoxic stress by combined activities core MAPK pathways, but how their signalling thresholds buffered an open question. Here we show mir-35-42 miRNA family play dual role antagonizing both NDK-1, positive regulator signalling,...
Organ development depends on multiple signaling pathways working in concert to specify cell fates. Improper activity or inactivity of specific such as EGF-Ras-MAPK can lead dedifferentiation and cancer. In C. elegans , gain function mutations Ras/ let-60 ectopic ventral vulva-like lesions resembling tumors. However, this phenotype normal insulin-like growth factor (IGF) signaling. Here, we probe how factors downstream the IGF receptor daf-2 modify Ras These investigations led us identify...
Abstract Highly efficient generation of deletions, substitutions, and small insertions (up to ∼150 bp) into the C. elegans genome by CRISPR/Cas9 has been facilitated use single-stranded oligonucleotide donors as repair templates. However, insertion larger sequences such fluorescent markers other functional proteins remains inefficient due lack standardized methods for generating templates labor intensive or cost prohibitive synthesis. We have optimized simple long DNA in using a standard PCR...