A5087117068- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Signaling Pathways in Disease
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
Pennsylvania State University
2022-2025
Penn State Milton S. Hershey Medical Center
2022-2023
Nucleosome chains fold and self-associate to form higher-order structures whose internal organization is unknown. Here, cryoelectron tomography (cryo-ET) of native human chromatin reveals intrinsic folding motifs such as (1) non-uniform nucleosome stacking, (2) intermittent parallel perpendicular orientations adjacent planes, (3) a regressive chain path, which deviates from the direct zigzag topology seen in reconstituted nucleosomal arrays. By examining self-associated structures, we...
The formation of condensed heterochromatin is critical for establishing cell-specific transcriptional programs. To reveal structural transitions underlying in maturing mouse rod photoreceptors, we apply cryo-EM tomography, AI-assisted deep denoising, and molecular modeling. We find that chromatin isolated from immature retina cells contains many closely apposed nucleosomes with extremely short or absent nucleosome linkers, which are inconsistent the typical two-start zigzag folding. In...
SUMMARY Nucleosome chains fold and self-associate to form higher order structures whose internal organization is unknown. Here, cryo-electron tomography (cryo-ET) of native human chromatin reveals novel folding motifs such as 1) non-uniform nucleosome stacking, 2) intermittent parallel perpendicular orientations adjacent planes, 3) an inverse zigzag chain path, which deviates from the direct topology seen in reconstituted nucleosomal arrays. By examining these self-associated structures, we...
Nucleosome chains fold and self-associate to form higher order structures whose internal organization is unknown. Here, cryo-electron tomography (cryo-ET) of native human chromatin reveals novel folding motifs such as 1) non-uniform nucleosome stacking, 2) intermittent parallel perpendicular orientations adjacent planes, 3) an inverse zigzag chain path, which deviates from the direct topology seen in reconstituted nucleosomal arrays. By examining these self-associated structures, we observed...