A5073566367- Animal Behavior and Reproduction
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Invertebrate Immune Response Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant and animal studies
- Insect symbiosis and bacterial influences
- Sperm and Testicular Function
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Insect Resistance and Genetics
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Nuclear Structure and Function
- Genetic diversity and population structure
- Plant Reproductive Biology
- Physiological and biochemical adaptations
- Mosquito-borne diseases and control
- Chromosomal and Genetic Variations
- Insect Utilization and Effects
- Insect and Pesticide Research
- Heat shock proteins research
- Signaling Pathways in Disease
- Animal Genetics and Reproduction
Cornell University
2016-2025
Pediatrics and Genetics
2014-2023
University of Oslo
2022
National Heart Lung and Blood Institute
2022
University of Washington
2022
New York University
2022
University of Southern California
2022
Institut thématique Génétique, génomique et bioinformatique
2015
Ithaca College
2012-2015
University of California, San Diego
1985-2009
Sequence comparisons of genomes or expressed sequence tags (ESTs) from related organisms provide insight into functional conservation and diversification. We compare the sequences ESTs male accessory gland Drosophila simulans to their orthologs in its close relative melanogaster , demonstrate rapid divergence many these reproductive genes. Nineteen (∼11%) 176 independent genes identified EST screen contain protein-coding regions with an excess nonsynonymous over synonymous changes,...
Mating induces profound changes in female insect behavior and physiology. In Drosophila melanogaster , mating causes a reduction sexual receptivity an elevation egg production for at least 5 days. Injection of the seminal fluid sex peptide (SP) both responses virgin females, but only 1–2 The role SP eliciting to remains be elucidated. Functional redundancy between components may occur. addition, with spermless males results brief (1- 2-day) post-mating responses, indicating either that there...
Rapid evolution driven by positive Darwinian selection is a recurrent theme in male reproductive protein evolution. In contrast, has never been demonstrated for female proteins. Here, we perform phylogeny-based tests on three mammalian fertilization proteins and demonstrate promoting their divergence. Two of these proteins, the zona pellucida glycoproteins ZP2 ZP3, are part egg coat. Several sites identified ZP3 as likely to be under located region previously involved species-specific...
Abstract A large portion of the annotated genes in Drosophila melanogaster show sex-biased expression, indicating that sex and reproduction-related (SRR genes) represent an appreciable component genome. Previous studies, which subsets were compared among few species, have found SRR exhibit unusual evolutionary patterns. Here, we used newly released genome sequences from 12 coupled to a larger set genes, comprehensively test generality these Among 2505 examined, including ESTs with biased...
Abstract Successful reproduction requires contributions from both the male and female. In Drosophila, include accessory gland proteins (Acps) that are components of seminal fluid. Upon their transfer to female, Acps affect female's physiology behavior. Although primary sequences Acp genes exhibit variation among species genera, conservation protein biochemical classes in fluid suggests a functions. Bioinformatics coupled with molecular genetic tools available for Drosophila melanogaster has...
Mating triggers behavioral and physiological changes in the Drosophila melanogaster female, including an elevation of egg laying. Seminal fluid molecules from male accessory gland are responsible for initial changes, but persistence these requires stored sperm. Using genetic analysis, we have identified a seminal protein that is This molecule, Acp26Aa, has structural features prohormone contains region with amino acid similarity to egg-laying hormone Aplysia. Acp26Aa transferred female...
Abstract Mated females of many animal species store sperm. Sperm storage profoundly influences the number, timing, and paternity female’s progeny. To investigate mechanisms for sperm in Drosophila melanogaster, we generated analyzed mutations Acp36DE. Acp36DE is a male seminal fluid protein whose localization mated suggested role storage. We report that male-derived essential efficient by females. Acp36DE1 (null) mutant males produced transferred normal amounts proteins. However, mates...
Abstract Genes whose products are involved in reproduction include some of the fastest-evolving genes found within genomes several organisms. Drosophila has long been used to study function and evolutionary dynamics thought be sperm competition sexual conflict, two processes that have hypothesized drive adaptive evolution reproductive molecules. Several seminal fluid proteins (Acps) made male tract show evidence rapid evolution. To identify candidate female may female–male interactions thus...
The female Drosophila melanogaster fly undergoes behavioral changes after mating, including an increase in egg laying and avoidance of remating. Accessory-gland products elicit these transiently when introduced into unmated flies. We report here the generation phenotype flies that lack functional accessory-gland main cells as a consequence genetically directed delivery diphtheria toxin subunit A to those cells. Only main-cell secretions are essential for short-term inhibition remating; no...
Successful reproduction is critical to pass genes the next generation. Seminal proteins contribute important reproductive processes that lead fertilization in species ranging from insects mammals. In Drosophila, male's accessory gland a source of seminal fluid affect output males and females by altering female post-mating behavior physiology. Protein classes found Drosophila are similar those other organisms, including By using RNA interference (RNAi) knock down levels individual (Acps), we...
Seminal fluid proteins transferred from males to females during copulation are required for full fertility and can exert dramatic effects on female physiology behavior. In Drosophila melanogaster, the seminal protein sex peptide (SP) affects mated by increasing egg production decreasing receptivity courtship. These behavioral changes persist several days because SP binds sperm that stored in female. is then gradually released, allowing it interact with its female-expressed receptor. The...
Abstract Drosophila melanogaster males transfer seminal fluid proteins along with sperm during mating. Among these proteins, ACPs (Accessory gland proteins) from the male's accessory induce behavioral, physiological, and life span reduction in mated females mediate storage utilization. A previous evolutionary EST screen D. simulans identified partial cDNAs for 57 new candidate ACPs. Here we report annotation confirmation of corresponding Acp genes melanogaster. Of previously reported...