A5058114045- Ion Channels and Receptors
- Ion channel regulation and function
- Pain Mechanisms and Treatments
- Plant Stress Responses and Tolerance
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Piperaceae Chemical and Biological Studies
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Respiratory and Cough-Related Research
- Magnesium in Health and Disease
- Phytochemicals and Antioxidant Activities
Philipps University of Marburg
2013-2024
Humboldt-Universität zu Berlin
2023
Charité - Universitätsmedizin Berlin
2022-2023
Freie Universität Berlin
2023
Justus-Liebig-Universität Gießen
2020
Saarland University
2012-2016
Opioids, agonists of µ-opioid receptors (µORs), are the strongest pain killers clinically available. Their action includes a strong central component, which also causes important adverse effects. However, µORs found on peripheral endings nociceptors and their activation there produces meaningful analgesia. The cellular mechanisms downstream not well understood. Here, we show in neurons murine dorsal root ganglia that pro-nociceptive TRPM3 channels, present parts nociceptors, strongly...
TRPM3 channels form ionotropic steroid receptors in the plasma membrane of pancreatic β and dorsal root ganglion cells link hormone signaling to insulin release pain perception, respectively. We identified compared function a number splice variants present mouse, rat human tissues. found that lacking region 18 amino acid residues display neither Ca2+ entry nor ionic currents when expressed alone. Hence, splicing removes is indispensable for channel function, which called ICF region. devoid...
Transient receptor potential melastatin 3 (TRPM3) proteins form non-selective but calcium-permeable membrane channels, rapidly activated by extracellular application of the steroid pregnenolone sulphate and dihydropyridine nifedipine. Our aim was to characterize binding site analysing structural chemical requirements for TRPM3 activation.
Background and Purpose Signalling through phospholipase C (PLC) controls many cellular processes. Much information on the relevance of this important pathway has been derived from pharmacological inhibition enzymatic activity PLC. We found that most frequently employed PLC inhibitor, U73122, activates endogenous ionic currents in widely used cell lines. Given extensive use U73122 research, we set out to identify these U73122‐sensitive ion channels. Experimental Approach performed detailed...
TRPM3 channels play important roles in the detection of noxious heat and inflammatory thermal hyperalgesia. The activity these ion somatosensory neurons is tightly regulated by µ-opioid receptors through signaling Gβγ proteins, thereby reducing TRPM3-mediated pain. We show here that directly binds to a domain 10 amino acids solve cocrystal structure this together with Gβγ. Using data mutational analysis full-length we pinpoint three their interacting partners Gβ
N-(3-fluoro-1-phenethylpiperidine-4-yl)-N-phenyl propionamide is a newly-designed pain killer selectively activating G-protein-coupled mu-opioid receptors (MOR) in acidic injured tissues, and therefore devoid of central side effects which are typically elicited at normal pH values healthy tissues. However, the neuronal mechanisms underlying NFEPP’s antinociceptive were not examined detail so far. Voltage-dependent Ca 2+ channels (VDCCs) nociceptive neurons play major role generation...
Abstract Background and Purpose NFEPP is a newly-designed pain killer selectively activating G-protein coupled mu opioid receptors in injured tissues, therefore devoid of central side effects. However, the cellular mechanisms underlying NFEPP’s antinociceptive effects were not examined sufficient detail so far. Here we investigated on activation, voltage gated calcium channels receptor phosphorylation. Experimental Approach HEK293 cells stably transfected with used to study [35S]-GTPγS...