A5026450526- Adenosine and Purinergic Signaling
- Neurological Complications and Syndromes
- Nitric Oxide and Endothelin Effects
- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Agriculture Sustainability and Environmental Impact
- Angiogenesis and VEGF in Cancer
- Cancer, Lipids, and Metabolism
- Diabetes Management and Research
- Neuroscience of respiration and sleep
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Diabetes Treatment and Management
- Retinal Diseases and Treatments
- Renal and Vascular Pathologies
- Biomedical Research and Pathophysiology
- Lipoproteins and Cardiovascular Health
- Bipolar Disorder and Treatment
- Diet and metabolism studies
- Paraoxonase enzyme and polymorphisms
- Blood Pressure and Hypertension Studies
- Phytoestrogen effects and research
- Diabetes and associated disorders
- Pregnancy and Medication Impact
- Advanced Glycation End Products research
- Natural Antidiabetic Agents Studies
Gdańsk Medical University
2012-2024
Mossakowski Medical Research Institute, Polish Academy of Sciences
2019
Polish Academy of Sciences
2019
The aim of the study was to investigate effect a vegan diet on serum lipid profile with particular regard parameters characterizing high-density lipoprotein (HDL) fractions in subjects without subclinical atherosclerosis, measured by carotid Doppler ultrasonography.Forty-two 23 38 year old (21 omnivores and 21 vegans) participated study. Compared omnivores, were characterized lower profile: total cholesterol (p < 0.001), low-density (LDL)-cholesterol non-HDL-cholesterol apolipoprotein B...
Hypercholesterolemia-associated oxidative stress increases the formation of oxidized low-density lipoprotein (oxLDL), which can affect endothelial cell function and potentially contribute to renal dysfunction, as reflected by changes in urinary protein excretion. This study aimed investigate impact exogenous oxLDL on excretion albumin nephrin. LDL was isolated from a patient with familial hypercholesterolemia (FH) undergoing apheresis (LA) vitro Cu (II) ions. Biochemical markers oxidation,...
Purinoceptors (adrengeric receptors and P2 receptors) are expressed on the cellular components of glomerular filtration barrier, their activation may affect permeability to albumin, which ultimately lead albuminuria, a well-established risk factor for progression chronic kidney disease development cardiovascular diseases. We investigated mechanisms underlying in vitro vivo purinergic actions filter albumin by measuring convectional ( P alb ) single isolated rat glomerulus based video...
Being rich in polyunsaturated fatty acids, flaxseed (Linum usitatissimum L.) is thought to be able decrease lipid levels and dampen inflammation. In this pilot study, we aimed determine whether supplementation could improve the profiles of lipids inflammatory mediators patients with severe hyperlipidemia resistant conventional lipid-lowering pharmacotherapy requiring lipoprotein apheresis. To end, six received, blindly—in addition their normal apheresis regimen—a 10-week dietary (28 g/d)...
BackgroundKidney noradrenergic innervation regulates tubular function. Adenosine triphosphate (ATP)—a co-transmitter of norepinephrine—acts on purinoceptors, including ion channel receptor, P2X. P2X receptor agonists, α,β-methylene ATP (α,β-meATP) and β,γ-methylene (β,γ-meATP), induce natriuresis. Regarding the functional co-localization adrenoceptors receptors, we evaluated rat renal system sensitivity to natriuretic action agonists in chronically denervated kidney.
Lipoprotein apheresis (LA) treatment results in a substantial reduction of low-density lipoprotein- (LDL-) cholesterol and lipoprotein(a) concentrations, which consequently decreases the rate cardiovascular events. The additional benefit LA may be associated with its impact on composition quality high-density lipoprotein (HDL) particles, inflammation, oxidative stress condition. To verify effects procedure, current study is aimed at analyzing effect single procedure direct hemadsorption...
Diabetic nephropathy (DN) accounts for approximately 50% of end-stage renal diseases. Vascular endothelial growth factor A (VEGF-A) is thought to be a critical mediator vascular dysfunction in DN, but its role unclear. The lack pharmacological tools modify concentrations further hinders the understanding DN. In this study, rats were evaluated after 3 weeks streptozotocin-induced diabetes and two suramin treatments (10 mg/kg, ip). expression was by western blot glomeruli immunofluorescence...
Diabetes mellitus causes changes in metabolism of extracellular nucleotides acting through P2 receptors (P2Rs). This affects renal function and may lead to glomerular tubular disturbances. We measured urinary excretion (ATP, ADP, AMP, UTP, UDP, UMP) streptozotocin-induced diabetic rats (65 mg/kg, i.p., day 0) the effects P2Rs' blockade by suramin (10 days +7, +14) on P2×7R expression (albumin, nephrin) (KIM-1, NGAL) injury markers, electrolytes, oxidative stress markers (TBARS, 8-OHdG)....
Diabetes through adenosine A1 receptor (A<sub>1</sub>R) and P2 receptors (P2Rs) may lead to disturbances in renal microvasculature. We investigated the microvascular response Ap<sub>4</sub>A, an agonist of P2Rs, streptozotocin-induced diabetic rats. Using laser Doppler flowmetry, blood perfusion (RBP) was measured during infusion Ap<sub>4</sub>A alone or presence A<sub>1</sub>R antagonist, either DPCPX (8-cyclopentyl-1,3-dipropylxanthine)...
Purinergic receptors are expressed on cellular components of glomerular filtration barrier and their activation may affect permeability for albumin leading to albuminuria. We investigated mechanisms underlying purinergic action filter measuring convectional (P alb ) in single isolated glomerulus based video‐microscopy method. Effect nucleotides vivo was using osmotic pump implanted subcutaneously into Wistar rats. Cultured rat podocytes were used analysis permeability, PKG dimerization...
In short-term diabetes (3 weeks), suramin, a drug used clinically, affects renal function and the expression of vascular endothelial growth factor A (VEGF-A), which may be involved in pathogenesis diabetic nephropathy, main cause end-stage disease. present study, we evaluated long-term (11 weeks) effects suramin (10 mg/kg, i.p., once-weekly) rats. Concentrations VEGF-A, albumin, soluble adhesive molecules (sICAM-1, sVCAM-1), nucleosomes, thrombin-antithrombin complex (TAT) were measured by...
Abstract Background and Aims Extracellular nucleotides (ATP, ADP, UTP, UDP) acting through P2-receptors regulate renal vascular tone glomerular permeability tor albumin. Both phenomenons are dysregulated in diabetes conducive to development of diabetic nephropathy. The aim study was investigate the urinary excretion adenylate uridynate effects suramin, antagonist non-competitive inhibitor ectoATPase, on those parameters streptozotocin-induced rats. Method Diabetes Wistar rats induced by...
Hypercholesterolemia and oxidative stress may lead to disturbances in the renal microvasculature response vasoactive agents, including P2 receptors (P2R) agonists. We investigated microvascular diadenosine tetraphosphate (Ap4 A), an agonist of P2R, diet-induced hypercholesteremic rats over 28 days, supplemented last 10 days with tempol (2 mM) or DL-buthionine-(S,R)-sulfoximine (BSO, 20 drinking water. Using laser Doppler flowmetry, blood perfusion cortex medulla (CBP, MBP) was measured...