MicroRNAs (miRNAs) are critical regulators of gene expression in healthy and diseased states, numerous studies have established their tremendous potential as a tool for improving the diagnosis Type 2 Diabetes Mellitus (T2D) its comorbidities. In this regard, we computationally identify novel top-ranked hub miRNAs that might be involved T2D. We accomplish via two strategies: 1) by ranking based on number T2D differentially expressed genes (DEGs) they target, 2) using only common DEGs between...

Some unidentified RNA molecules, together with the nucleoid protein HU, were suggested to be involved in structure of Escherichia coli. HU is a conserved known for its role binding DNA and maintaining negative supercoils latter. also binds few RNAs, but full spectrum targets cell not known. To understand any interaction structure, we immunoprecipitated potential HU-RNA complexes from cells examined bound RNAs by hybridization whole-genome tiling arrays. We identified associations between 10...

Repeated extragenic palindromes (REPs) in the enterobacterial genomes are usually composed of individual palindromic units separated by linker sequences. A total 355 annotated REPs distributed along Escherichia coli genome. RNA sequence (RNAseq) analysis showed that almost 80% E. transcribed. The DNA REP325 it is a cluster six repeats, each with two capable forming cruciform structures supercoiled DNA. Here, we report components element and at least one its products play role bacterial...

Reductions in growth rate caused by fusidic acid‐resistant EF‐G mutants Salmonella typhimurium correlate strongly with increased mean cell size. This is unusual because and size normally positively. The global transcription regulator molecule ppGpp has a role co‐ordinating division, its basal level correlates inversely at division. We show that have perturbed levels during steady‐state induced starvation. One mutation, fusA 1, associated the slowest largest size, causes reduction of to...

Mutants of Salmonella enterica serovar Typhimurium resistant to fusidic acid (Fusr) have mutations in fusA, the gene encoding translation elongation factor G (EF-G). Most Fusr mutants reduced fitness vitro and vivo, part explained by mutant EF-G slowing rate protein synthesis growth. However, some with normal rates still suffer from vivo. As shown here, could be similarly ranked their relative mouse infection models, a macrophage model, hypersensitivity hydrogen peroxide vivo vitro, amount...

Fusidic acid resistance (Fus(R)) in Salmonella enterica serovar Typhimurium is caused by mutations fusA, encoding elongation factor G (EF-G). Pleiotropic phenotypes are observed Fus(R) mutants. Thus, the fusA1 allele (EF-G P413L) associated with slow growth rate, reduced ppGpp and RpoS levels, heme increased sensitivity to oxidative stress. The fusA1-15 allele, P413L T423I) derived from a selection for rate compensation, partially compensated each of these phenotypic defects but maintains...

Background: Subacute thyroiditis (SAT) is an organ-specific disease that various drugs, including COVID-19 vaccines, can trigger. infection has been associated with thyroid gland damage and SARS-CoV-2 direct action, euthyroid sick syndrome, immune-mediated mechanisms are all potential of damage. It denotes inflammation, most commonly viral origin, belongs to the transitory, self-limiting diseases group, causing complications in approximately 15% patients form permanent hypothyroidism. Some...

Mutations in the translation elongation factor G (EF-G) make Salmonella enterica serovar Typhimurium resistant to antibiotic fusidic acid. Fus(r) mutants are hypersensitive oxidative stress and rapidly lose viability presence of hydrogen peroxide. We show that this phenotype is associated with reduced activity two catalase enzymes, HPI (a bifunctional catalase-hydroperoxidase) HPII monofunctional catalase). These catalases require iron-binding cofactor heme for their activity. have a rate...

Abstract: Timely and accurate evaluation of clinical parameters associated with endothelial dysfunction is critical in diagnosing treating atherosclerosis, which represents a severe health problem, accounting for at least 30% deaths globally. A early event the pathogenesis atherosclerosis oxidative modification low-density lipoprotein [LDL]. Oxidized LDL [OxLDL] numerous changes lipid apolipoprotein B [apo B] fractions LDLs generated by peroxidation. Another indicator perturbed vascular...

Diabetes mellitus (DM) refers to a complex cluster of metabolic disorders characterized by hyperglycemia caused inadequate insulin secretion, resistance, or excessive glucagon secretion. If not correctly treated, the prolonged effects DM-associated perturbations lead systemic vascular complications and cardiovascular disease (CVD), principal cause mortality among patients with DM. Given increase in global prevalence diabetes, novel diagnostic therapeutic procedures are necessary for its...

Thyroid nodules (TN) are localized morphological changes in the thyroid gland and can be benign or malignant.The present study investigates relationships between biochemical markers serum (s) their homologs washout (w) after fine-needle aspiration biopsy (FNAB) of TN interest correlation with cytology specimen findings.We investigated nitric oxide (NO), thyroglobulin (TG), calcitonin (CT), FNAB interest, findings samples classified according to Bethesda system for cytopathology this study,...

The responses of liver glucocorticoid receptor (GR) and genes coding for a glucocorticoid-inducible tyrosine aminotransferase (TAT) two acute-phase proteins (APP) [alpha2-macroglobulin (alpha2-M) gamma-fibrinogen (Fb)] to changes in (GC) proinflammatory (AP) cytokine contents have been examined rats after single or combined treatments with turpentine oil, dexamethasone (Dex) adrenalectomy. Activation APP turpentine-induced inflammation was accompanied by an increase the level GR mRNA...