A5058697690- Hearing, Cochlea, Tinnitus, Genetics
- Neuroscience and Neuropharmacology Research
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Receptor Mechanisms and Signaling
- Retinal Development and Disorders
- Circadian rhythm and melatonin
- Multisensory perception and integration
- Nicotinic Acetylcholine Receptors Study
- Olfactory and Sensory Function Studies
- Neural dynamics and brain function
- Hearing Loss and Rehabilitation
Northeast Ohio Medical University
2018-2024
Kent State University
2018-2020
Vanderbilt University
2012-2020
Arrestins belong to a family of multifunctional adaptor proteins that regulate internalization diverse receptors including G-protein-coupled (GPCRs). Defects associated with endocytosis GPCRs have been linked human diseases. We used enhanced green fluorescent protein-tagged arrestin 2 (Arr2) monitor the turnover major rhodopsin (Rh1) in live Drosophila. demonstrate during degeneration norpA(P24) photoreceptors loss Rh1 is parallel disappearance rhabdomeres, specialized visual organelle...
Arrestin regulates many facets of G-protein coupled receptor signaling. In Drosophila, 1 (Arr1) is expressed at a lower level than 2 (Arr2), and the role Arr1 in visual physiology less understood. Here we generated transgenic flies expressing enhanced green fluorescent protein tagged (Arr1-eGFP) explored its trafficking live photoreceptors. We show that Arr1-eGFP localized cytoplasm displays light-dependent translocation to rhabdomere possibly by interacting with photoactivated rhodopsin...
Unregulated Ca2+ influx affects intracellular homoeostasis, which may lead to neuronal death. In Drosophila, following the activation of rhodopsin TRP channel is open mediate light-dependent depolarization. A constitutively active triggers degeneration TrpP365 /+ photoreceptors. To explore retinal degeneration, we employed a multidisciplinary approach including live imaging using GFP tagged actin and arrestin 2. Importantly, demonstrate that major (Rh1) was greatly reduced before onset...
Little is known about the functions of Group II metabotropic glutamate receptors (mGluRs2/3) in inferior colliculus (IC), a midbrain structure that major integration region central auditory system. We investigated how these modulate sound-evoked and spontaneous firing mouse IC vivo first performed immunostaining tested hearing thresholds to validate vesicular GABA transporter (VGAT)-ChR2 transgenic mice on mixed CBA/CaJ x C57BL/6J genetic background. Transgenic animals allowed for...
Abstract Little is known about the functions of group II metabotropic glutamate receptors (mGluRs2/3) in inferior colliculus (IC)—a midbrain structure that a major integration region central auditory system. We investigated how these modulate sound-evoked and spontaneous firing mouse IC vivo . first performed immunostaining tested hearing thresholds to validate VGAT-ChR2 transgenic mice on mixed CBA/CaJ x C57BL/6J genetic background. Transgenic animals allowed for optogenetic cell type...