A5006413335- Pesticide and Herbicide Environmental Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Escherichia coli research studies
- Microbial Community Ecology and Physiology
- Viral gastroenteritis research and epidemiology
- Soil Carbon and Nitrogen Dynamics
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Microbial bioremediation and biosurfactants
- Environmental Toxicology and Ecotoxicology
- Analytical chemistry methods development
- Pesticide Residue Analysis and Safety
- Agricultural safety and regulations
- Entomopathogenic Microorganisms in Pest Control
- Phenothiazines and Benzothiazines Synthesis and Activities
- Diatoms and Algae Research
- Nematode management and characterization studies
- Chemistry and Chemical Engineering
- Wastewater Treatment and Nitrogen Removal
- Insect Resistance and Genetics
- Weed Control and Herbicide Applications
- Environmental DNA in Biodiversity Studies
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Pesticide Exposure and Toxicity
Laboratoire Microorganismes Génome et Environnement
2015-2024
Centre National de la Recherche Scientifique
2009-2023
Université Clermont Auvergne
2010-2023
Université de Montréal
2003-2006
Centre de Recherche en Nutrition Humaine d'Auvergne
2000
Institut National de la Recherche Agronomique
2000
ABSTRACT Nonenterotoxigenic porcine Escherichia coli strains belonging to the serogroup O45 have been associated with postweaning diarrhea in swine and adhere intestinal epithelial cells a characteristic attaching effacing (A/E) pattern. enteropathogenic E. (PEPEC) strain 86-1390 induces typical A/E lesions pig ileal explant model. Using Tn phoA transposon insertion mutagenesis on 86-1390, we found mutant that did not induce lesions. The was identified gene designated paa (porcine...
The ileal in vitro organ culture (IVOC) model using tissues originating from colostrum-deprived newborn piglets has proven to be an effective way study the attaching and effacing (A/E) phenotype of porcine enteropathogenic Escherichia coli (EPEC) ex vivo. aim this was investigate role intimin subtype Tir adherence EPEC Shiga-toxin-producing E. (STEC), isolated different animal species, intestinal IVOC. Moreover, Tir-independent human strain E2348/69 investigated Tir-deficient derivatives....
ABSTRACT Studying aquatic microalgae is essential for monitoring biodiversity and water quality. We designed new sets of 18S rRNA PCR primers Chlorophyceae Bacillariophyceae by using the ARB software implementing a virtual program. The results specificity analysis showed that most targeted algal families were identified nontargeted organisms, such as fungi or ciliates, excluded. These newly developed primer also able to amplify microalgal genes from environmental samples with accurate...
Variovorax sp. strain WDL1, which mineralizes the phenylurea herbicide linuron, expresses a novel linuron-hydrolyzing enzyme, HylA, that converts linuron to 3,4-dichloroaniline (DCA). The enzyme is distinct from hydrolase LibA recently identified in other linuron-mineralizing strains and phenylurea-hydrolyzing enzymes (PuhA, PuhB) found Gram-positive bacteria. dimeric belongs separate family of hydrolases differs Km, temperature optimum, substrate range. Within metal-dependent amidohydrolase...
Abstract. In the context of ecological sustainability phosphorus, emerging evidence for ubiquitous presence polyphosphate-accumulating bacteria in natural environments invites efforts to reveal their roles biogeochemical cycle phosphorus. This requires high-throughput methods characterize structure and dynamics ecosystems. A promising strategy is combine staining intracellular polyphosphate granules subsequent detection by flow cytometry, enabling rapid data acquisition. this study, we...
Mesotrione is a selective herbicide belonging to the triketone family, commonly used on maize cultures since 2003. A mesotrione-transforming Bacillus megaterium Mes11 strain isolated from an agricultural soil was as model identify key enzymes initiating biotransformation of this herbicide. Two (called NfrA1 and NfrA2/YcnD) were identified, functionally structurally characterized. Both belong NfsA FRP family nitro-FMN reductase superfamily (type I oxygen-insensitive nitroreductase) show...
ABSTRACT The virulence genotype profile and presence of a pathogenicity island(s) (PAI) were studied in 18 strains F165-positive Escherichia coli originally isolated from diseased calves or piglets. On the basis their adhesion phenotypes genotypes, these extraintestinal pathogenic classified into three groups. F165 fimbrial complex consists at least two serologically genetically distinct fimbriae: 1 2 . is encoded by foo operon ( pap -like), fot sfa related). Strains group positive, afa 3...
A culture-dependent study was performed with the aim of assessing carbon, electron and Fe(III) sources used for dissimilatory reduction pathway diversity culturable Fe(III)-reducers in anoxic zone meromictic Lake Pavin. This metabolic investigated enrichment cultures inoculated water samples collected at 70 m depth Combinations different media, organic acids, incubation gas phases were performed. The potential growth conditions assessed by measuring accumulation Fe(II) overtime. Bacterial...
Nicosulfuron is a selective herbicide belonging to the sulfonylurea family, commonly applied on maize crops. Its worldwide use results in widespread presence as contaminant surface streams and ground-waters. In this study, we isolated, for first time, Plectosphaerella cucumerina AR1 nicosulfuron-degrading fungal strain, new record from Alnus leaf litter submerged freshwater. The degradation of nicosulfuron by P. was achieved co-metabolism process followed first-order model dissipation....
Using a porcine ileal in vitro organ culture model, we have demonstrated that egg yolk-derived antibodies specific for the attaching and effacing Escherichia coli (AEEC) virulence factors intimin translocated receptor (Tir), but not those AEEC-secreted proteins EspA, EspB EspD, significantly reduced bacterial adherence of enteropathogenic E. strain ECL1001, formerly 86-1390. Moreover, Tir also heterologous AEEC strains, including human, bovine canine as well O157:H7 Shiga toxin-producing...
In order to reduce the amounts of pesticides used, and thereby their associated risks, new generations less environmentally dangerous molecules with lower weight are currently being used in mixtures sprayed on crops. Few studies have been made, however, analyse impact soil, more particularly microorganisms living soil which maintain essential functions this ecosystem. By taking a microcosmic approach, we were able assess maize herbicides “cocktail” Mesotrione S-metolachlor global microbial...