A5101798050- Blood Coagulation and Thrombosis Mechanisms
- Zebrafish Biomedical Research Applications
- Genetic Associations and Epidemiology
- Fish Ecology and Management Studies
- Venous Thromboembolism Diagnosis and Management
- Genetic and phenotypic traits in livestock
- Platelet Disorders and Treatments
- Mosquito-borne diseases and control
- Hemophilia Treatment and Research
University of Michigan
2021-2024
University of Toledo
2022
Michigan United
2022
SUMMARY Genome wide association study (GWAS) results for Venous Thromboembolism (VTE) across 9 international cohorts of the Global Biobank Meta-analysis Initiative (GBMI), with representation six ancestry groups (cases=27,987, controls=1,035,290), were combined using inverse-variance weighted meta-analysis. This multi-ancestry GWAS resulted in 38 genome-wide significant loci, which are potentially novel. For each autosomal locus we performed gene prioritization seven independent, yet...
Tissue factor (TF) is an evolutionarily conserved protein necessary for initiation of hemostasis. Zebrafish have two copies the tissue gene ( f3a and f3b ) as result ancestral teleost fish duplication event (so called ohnologs). In vivo physiologic studies TF function been difficult given early lethality knockout in mouse. We used genome editing to produce knockouts both zebrafish. Since ohnologs arose through sub- or neofunctionalization, they can unmask unknown functions non-teleost genes...
Venous thrombosis is a leading cause of morbidity/mortality and associated with deficiencies the anticoagulant protein C (PC; PROC) its cofactor, S (PS; PROS1). Heterozygous mutations increase risk adult-onset thrombosis, whereas homozygous result in pre/neonatal lethal thrombosis. Phenotypes patients PC PS deficiency are generally considered clinically indistinguishable. Here, we generate proc (zebrafish PROC ortholog) pros1 knockouts through genome editing zebrafish uncover partially...