A5010890755- Birth, Development, and Health
- Prostate Cancer Treatment and Research
- Neonatal Respiratory Health Research
- Prostate Cancer Diagnosis and Treatment
- Congenital Heart Disease Studies
- Congenital heart defects research
- Cardiovascular Issues in Pregnancy
- Transplantation: Methods and Outcomes
- Cardiomyopathy and Myosin Studies
- Pulmonary Hypertension Research and Treatments
- Pregnancy and preeclampsia studies
- Global Cancer Incidence and Screening
- Prenatal Substance Exposure Effects
- Organ Transplantation Techniques and Outcomes
- Endometrial and Cervical Cancer Treatments
- Genomics and Chromatin Dynamics
- Chronic Kidney Disease and Diabetes
- Fatty Acid Research and Health
- Urinary Bladder and Prostate Research
- Cancer survivorship and care
- LGBTQ Health, Identity, and Policy
- Cardiovascular Conditions and Treatments
- Sexual Differentiation and Disorders
- Renal Diseases and Glomerulopathies
- Cardiovascular Function and Risk Factors
Monash University
2011-2024
Australian Regenerative Medicine Institute
2018
Discovery Institute
2016
Pre-term birth affects 10-12% of live births and occurs when the myocardium is still developing; therefore, final structure could be altered. We hypothesized that, in response to pre-term birth, structural remodelling within which enables immature heart muscle adapt haemodynamic transition at but results persistent alterations its structure. Our objective was determine how alters myocardium.Using sheep, induced 0.9 term; hearts were examined 9 weeks after term-equivalent age, cardiomyocyte...
Abstract Quantitative assessment of myocardial development and disease requires accurate measurement cardiomyocyte volume, nuclearity (nuclei per cell), ploidy (genome copies cell). Current methods require enzymatically isolating cells, which excludes the use archived tissue, or serial sectioning. We describe a method analysis that permits direct simultaneous nuclearity, in thick histological sections. To demonstrate utility our technique, heart tissue was obtained from four species (rat,...
Podocyte depletion is sufficient for the development of numerous glomerular diseases and can be absolute (loss podocytes) or relative (reduced number podocytes per volume glomerulus). Commonly used methods to quantify podocyte introduce bias, whereas gold standard stereologic methodologies are time consuming impractical. We developed a novel approach assessing in whole glomeruli that combines immunofluorescence, optical clearing, confocal microscopy, three-dimensional analysis. validated...
Prenatal exposure to high levels of ethanol is associated with cardiac malformations, but the effects lower on heart are unclear. Our aim was investigate daily during late gestation, when cardiomyocytes undergoing maturation, developing myocardium. Pregnant ewes were infused either (0.75 g/kg) or saline for 1 h each day from gestational days 95 133 (term ∼145 days); tissues collected at 134 days. In sheep, mature gestation as in humans. Within left ventricle (LV), cardiomyocyte number...
30 day mortality in patients with Acute Respiratory Failure (ARF) is approximately 30%, defined as requiring ventilator support for more than 6 hours. Novel biomarkers are needed to predict patient outcomes and guide potential future therapies. The activins A B, members of the Transforming Growth Factor β family proteins, their binding protein, follistatin, have recently been shown be important regulators inflammation fibrosis but no substantial data available concerning roles ARF.Specific...
The Australia and New Zealand Prostate Cancer Outcomes Registry (PCOR-ANZ) has now entered its ninth year of operation since establishment in 2015 [1]. PCOR-ANZ represents a unique, huge, rich dataset which can, should, be optimally utilised to monitor, benchmark, improve the quality prostate cancer care (ANZ) [2]. 2023 Annual Report was recently published, detailing activities for period 2015–2021 [3]. Here, we provide summary key findings plans moving forward. Between January December...
Intrauterine inflammation is a major contributor to preterm birth and has adverse effects on neonatal cardiovascular physiology. Cardiomyocyte maturation occurs in late gestation species such as humans sheep. We tested the hypothesis that intrauterine deleterious cardiac function sheep which might be explained by altered cardiomyocyte proliferation maturation. Pregnant ewes received an ultrasound-guided intra-amniotic injection of lipopolysaccharide (LPS) or saline 7 days prior delivery at...
The integrity of male germ cell genome is critical for the correct progression spermatogenesis, successful fertilization, and proper development offspring. Several DNA repair pathways exist in cells. However, unlike somatic cells, key components such remain largely unidentified. Gametogenetin (GGN) a testis-enriched protein that has been shown to bind FANCL via yeast-two-hybrid assays. This finding its expression pattern raise possibility GGN plays role during spermatogenesis. Herein we...
Key points Preterm birth occurs when the heart muscle is immature and ill‐prepared for changes in lung function at birth. MRI imaging studies show differences growth of young adults born preterm, with effects more pronounced right ventricle. The findings this study, conducted sheep, showed that following moderate preterm ventricular wall was thinner adulthood, a reduction number size cells; addition, there impaired blood flow main artery leading from ventricle to lungs. indicate being only...
Abstract Background Antenatal conditions that are linked with preterm birth, such as intrauterine inflammation, can influence fetal cardiac development thereby rendering the heart more vulnerable to effects of prematurity. We aimed investigate effect consequent lipopolysaccharide exposure, on postnatal growth and maturation in lambs. Methods Preterm lambs (~129 days gestational age) exposed antenatally or saline were managed according contemporary neonatal care studied at day 7. Age-matched...
High levels of alcohol (ethanol) exposure during fetal life can affect liver development and increase susceptibility to infection after birth. Our aim was determine the effects a moderate level ethanol in late gestation on morphology, iron status, inflammatory status ovine liver. Pregnant ewes were chronically catheterized at 91 days (DG; term ∼145 DG) for daily intravenous infusion (0.75 g/kg maternal body wt; n = 8) or saline ( 7) over 1 h from 95 133 DG. At necropsy (134 DG), livers...
Background Lung transplantation exposes the donated lung to a period of anoxia. Re-establishing circulation after ischemia stimulates inflammation causing organ damage. Since our published data established that activin A is key pro-inflammatory cytokine, we assessed roles and B, their binding protein, follistatin, in patients undergoing transplantation. Methods Sera from 46 participating study remote conditioning were used. Serum follistatin 11 other cytokines measured samples taken...
Postnatal corticosteroids are used in the critical care of preterm infants for prevention and treatment bronchopulmonary dysplasia. We aimed to investigate effects early postnatal dexamethasone therapy dose on cardiac maturation morphology lambs.Lambs were delivered prematurely at ~128 days gestational age managed postnatally according best clinical practice. Preterm lambs administered daily either a low-dose (n = 9) or high-dose 7), naïve steroid saline 9), over 7-day time-course. Hearts...
Abstract Objective Feeling depressed and lethargic are common side effects of prostate cancer (PCa) its treatments. We examined the incidence severity feeling lack energy in patients a population based PCa registry. Methods included men diagnosed with between 2015 2019 Victoria, Australia, enrolled Prostate Cancer Outcomes Registry. The primary outcome measures were responses to two questions on Expanded Index Composite (EPIC‐26) patient reported instrument: problems 12 months following...