A5083964749- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Genomics and Rare Diseases
- Cellular transport and secretion
- Genetic Associations and Epidemiology
- DNA and Biological Computing
- Carbohydrate Chemistry and Synthesis
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Lysosomal Storage Disorders Research
ETH Zurich
2019-2021
Understanding how to program biological functions into artificial DNA sequences remains a key challenge in synthetic genomics. Here, we report the chemical synthesis and testing of Caulobacter ethensis-2.0 ( C. eth-2.0 ), rewritten bacterial genome composed most fundamental cell. We rebuilt essential crescentus through process rewriting studied genetic information content at level its genes. Within 785,701-bp genome, used sequence reduce number encoded features from 6,290 799. Overall,...
Glucocerebrosidase (GBA) is a lysosomal β-glucosidase that degrades glucosylceramide. Its deficiency results in Gaucher disease (GD). We examined the effects of active site occupancy GBA on its structural stability. For this, we made use cyclophellitol-derived activity-based probes (ABPs) bind irreversibly to catalytic nucleophile (E340), and for comparison, used potent reversible inhibitor isofagomine. demonstrate cyclophellitol ABPs improve stability vitro, as revealed by thermodynamic...
Abstract Sequence rewriting enables low-cost genome synthesis and the design of biological systems with orthogonal genetic codes. The error-free, robust nucleotide sequences can be achieved a complete annotation gene regulatory elements. Here, we compare transcription in Caulobacter crescentus to from plasmid-borne segments synthesized C. ethensis 2.0 . This rewritten derivative contains an extensive amount supposedly neutral mutations, including 123’562 synonymous codon changes....