Fabrication of micropatterned surfaces to organize and control cell adhesion proliferation is an indispensable technique for cell-based technologies. Although several successful strategies creating cellular micropatterns on substrates have been demonstrated, a complex multistep process requirements special expensive equipment or materials limit their prevalence as general experimental tool. To circumvent these problems, we describe here novel facile fabrication method surface patterning by...

Positioning of cells in a desired pattern on substrate is an important technique for cell-based technologies, including the fundamental investigation cell functions, tissue-engineering applications, and fabrication biosensors arrays. Recently, inkjet printing was recognized as promising approach to creation cellular patterns substrates, it has been achieved by living or adhesive proteins. In this article, we created complex using albumin-based technique. Albumin cross-linked ethylene glycol...

Serum albumin is the most abundant protein in blood plasma. Albumin has ability to bind a wide variety of compounds including drugs, and cells as well proteins do not attach an albumin-coated surface. These properties are attractive for biomaterials utilized biomedical fields. In present study, we aimed prepare water-insoluble film possessing suitable flexibility native characteristics, such drug binding resistance cell adhesion. To confer with both water insolubility without losing searched...

Dopamine-releasing cells derived from embryonic stem (ESCs) are potentially valuable in cell transplantation therapy for Parkinson's disease. There have been many recent investigations of the induction dopamine-releasing mouse and primate ESCs. However, there major obstacles to application ESC progeny therapy, including host immune responses transplanted difficulty collecting culture dishes undamaged. To overcome these obstacles, present study, cynomolgus monkey ES (cESC) aggregates enclosed...

In vitro evaluation of anticancer drugs using cancer cells has long been performed for the development novel and selection effective different patients. Recent studies have suggested that tumor stromal affect drug sensitivity cells; however, most conventional culture systems lack cells. this study, we fabricated a multicomponent coculture system takes account cancer-stroma interactions evaluation. system, small-cell nonsmall-cell lung embedded in collagen gel were cocultured with two types...

The protein microswimmer, composed of serum albumin, magnetic nanoparticles, and cell-capturing parts, can capture cells, transport them towards target destinations, release at sites in a controllable manner.

We present a simple method for neural cell fate specification directly from mouse embryonic stem cells (ES cells) in serum-free conditions the absence of embryoid body formation. Dissociated ES were cultured media supplemented with vitamin B12 and heparin, but without any expensive cytokines. After 14 days culture, beta-tubulin type III (TuJ1) tyrosine hydroxylase (TH)-positive colonies detected by immunocytochemical examinations. In addition, specific gene analyses RT-PCR demonstrated...

Abstract Surface-induced thrombosis is a major complication in the development of blood-contacting medical devices. Serum albumin has ability to bind wide variety compounds, including drugs, and neither cells nor proteins adsorb an albumin-coated surface. These properties are useful for improving blood compatibility biomaterial surfaces. In present study, we prepared water-insoluble film by cross-linking pharmaceutical grade recombinant human serum aiming clinical applications, loaded with...

We discovered the unique cell adhesive properties of ultraviolet (UV)-irradiated albumin films. Albumin films prepared using a cross-linking reagent with epoxy groups maintained native properties, such as resistance to adhesion. Interestingly, varied depending upon UV irradiation time; specifically, adhesiveness increased until 2 h irradiation, when number attached film was similar that culture dishes, and then decreased 20 after which surface returned initial non-adhesive state. To...

Abstract Cell transplantation therapy using dopaminergic neurons derived from embryonic stem (ES) cells for the treatment of Parkinson's disease has been proposed as one major applications cell‐based therapy. However, low collection efficiency a culture dish and rejection after are expected to limit their future clinical applications. To overcome these problems, we examined induction neurogenis ES under free‐floating conditions microencapsulation obtained cell aggregates into an agarose...

Patterned cell culturing is one of the most useful techniques for understanding interaction between geometric conditions surrounding cells and their behaviors. The authors previously proposed a simple method patterning with an agarose gel microstructure fabricated by microcasting degassed polydimethylsiloxane (PDMS) mold. Although vacuum pressure produced from PDMS can drive highly viscous solution, influence solution viscosity on casting process unknown. This study investigated influences...

A functional substrate capable of regulating protein adsorption was prepared using a crosslinked albumin (cl-albumin) film for use in the fabrication micropatterns. The proteins with different characteristics onto cl-albumin film, including serum proteins, albumin, and lysozyme, investigated quartz crystal microbalance. results showed that surfaces coated are highly resistant to adsorption, regardless charge rigidity. In addition, this adsorption-resistance property can be easily converted...

Drug delivery systems comprising drug carriers capable of adhering to intestinal tissue have considerable potential realize more sophisticated systemic and topical treatments in the tract. The development innovative strategies for improving adhesion efficiency is high importance advancement this field. Herein, a novel approach achieving presented, where accessibility carrier surface its subsequent are promoted by utilizing thermodynamic tendency hydrophobic dispersion solvent, triacetin, be...