A5029809125- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Neuroethics, Human Enhancement, Biomedical Innovations
- Genetics and Neurodevelopmental Disorders
- Cardiovascular Effects of Exercise
- Genomics and Chromatin Dynamics
- Microfluidic and Bio-sensing Technologies
- Cardiac pacing and defibrillation studies
University of California, San Diego
2022-2024
Abstract The heart, which is the first organ to develop, highly dependent on its form function 1,2 . However, how diverse cardiac cell types spatially coordinate create complex morphological structures that are crucial for heart remains unclear. Here we integrated single-cell RNA-sequencing with high-resolution multiplexed error-robust fluorescence in situ hybridization resolve identity of develop human heart. This approach also provided a spatial mapping individual cells enables...
Heart failure is a leading cause of morbidity and mortality; yet gene regulatory mechanisms driving cell type-specific pathologic responses remain undefined. Here, we present the type-resolved transcriptomes, chromatin accessibility, histone modifications organization 36 non-failing failing human hearts profiled from 776,479 cells spanning all cardiac chambers. Integrative analyses revealed dynamic changes in type composition, programs organization, which expanded annotation cis-regulatory...