MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by post-transcriptional inhibition of mRNA translation. Dysregulation miRNAs, including circulating has been reported to play an important role in the development various diseases, fibrotic diseases. Aberrant miRNAs vitreous humor vitreoretinal diseased eyes reported. However, pattern present proliferative disease (PVD) patients, diabetic retinopathy (PDR), and vitreoretinopathy (PVR), remains unknown. To investigate...

Significance Retinal ischemia causes hypoxia-induced neovascularization that stimulates production of proangiogenic cytokines such as VEGF by the hypoxia-inducible transcription factors HIF-1 and HIF-2. Current therapies for ischemic retinal diseases target circulating but do not modulate activity control its production. We demonstrate inhibiting HIF-mediated with a peptide derived from intrinsically disordered protein CITED2, negative feedback regulator HIF-1α, reduces pathological...

Abstract In the retina, microglia are resident immune cells that essential for development and function. Retinal play a central role in mediating pathological degeneration diseases such as glaucoma, retinitis pigmentosa, age‐related neurodegeneration, ischemic retinopathy, diabetic retinopathy. Current models of mature human retinal organoids (ROs) derived from iPS cell (hiPSC) do not contain integrated into layers. Increasing cellular diversity ROs by including would more accurately...

The purpose of this study was to investigate whether fibrocytes participate in formation the fibrovascular membrane (FVM) patients with proliferative diabetic retinopathy (PDR).Vitreous fluid and FVM samples were obtained during vitrectomy PDR. Samples from macular hole or epiretinal used as controls. Vitreous subjected immunohistochemical analysis. In addition, cells isolated vitreous PDR control cultured serum-free medium. Fibrocytes identified among these by morphological analyses. We...

Abnormal subretinal neovascularization is a characteristic of vision-threatening retinal diseases, including macular telangiectasia (MacTel) and angiomatous proliferation (RAP). Subretinal neovascular tufts photoreceptor dysfunction are observed in very-low-density lipoprotein receptor (Vldlr–/–) mutant mice. These changes mirror those patients with MacTel RAP, but the pathogenesis largely unknown. In this study, we show that microglia were closely associated Vldlr–/– mice tissue from...

Abstract Retinal neovascularization (NV) is the major cause of severe visual impairment in patients with ischemic eye diseases. While it known that retinal microglia contribute to both physiological and pathological angiogenesis, molecular mechanisms by which these glia regulate NV have not been fully elucidated. In this study, we utilized a microglia‐specific Transforming Growth Factor‐β (Tgfβ) receptor knock out mouse model human iPSC‐derived examine role Tgfβ signaling activated during...

Abstract Purpose This retrospective observational study aimed to investigate the longitudinal changes in renal function and central macular thickness (CMT) their impact on visual outcomes during anti-vascular endothelial growth factor (anti-VEGF) therapy for diabetic edema (DME). Study design employed a analyzed data from treatment-naive patients with DME (62 cases, 100 eyes) receiving anti-VEGF 36 months. Baseline follow-up assessments were conducted at 12, 24, Methods Best corrected acuity...

Diabetic macular edema (DME) is a common cause of visual impairment in patients with diabetes. Although intravitreal anti-vascular endothelial growth factor (VEGF) injections were efficacious clinical trials, several exhibited poor response. This study aimed to compare features between who susceptible anti-VEGF for DME and those not. A single-center, retrospective 102 such was conducted (123 eyes; mean ± standard deviation age, 63.4 10.8 years; 57.8% males). Systemic ocular data, assessed at...

<title>Abstract</title> <bold>Purpose</bold>: Intraocular lens (IOL)-induced pigment dispersion syndrome (PDS) represents a significant clinical challenge. However, literature on treatment strategies based intraocular pressure (IOP) is limited. In this retrospective comparative analysis, we analyzed patients with IOL-induced PDS who underwent surgical treatment, focusing outcomes and preoperative IOP. <bold>Methods</bold>: Japanese glaucoma surgery for IOL-related were divided into the IOL...

Purpose: The purpose of this study was to determine the clinical efficacy low-frequency intravitreal ranibizumab treat macular edema due retinal vein occlusion (RVO). Patients and methods: This a retrospective examination cases that received for untreated RVO over period 12 months. Instead conventional three monthly injections, injections were given once during introductory period. If recurrence diagnosed visit, additional as needed. There 21 eyes patients with branch (BRVO) ten central...

Disruption of the neurovascular unit (NVU) underlies pathophysiology various CNS diseases. One strategy to repair NVU dysfunction uses stem/progenitor cells provide trophic support NVU's functionally coupled and interdependent vasculature surrounding parenchyma. A subset endothelial progenitor cells, colony-forming (ECFCs) with high expression CD44 hyaluronan receptor (CD44hi), provides such neurovasculotrophic via a paracrine mechanism. Here, we report that bioactive extracellular vesicles...

Abstract Purpose Diabetic retinopathy leads to vision-threatening complications, such as proliferative diabetic and tractional retinal detachment (TRD) is a major global health concern. Despite advancements in vitrectomy techniques, challenges exist managing postoperative complications long-term visual acuity. This study aimed evaluate outcomes of 27-gauge pars plana (27 g PPV) for TRD identify associated risk factors. Study Design Retrospective study. Methods included 94 eyes 74 patients...

Diabetic retinopathy (DR) is the leading cause of visual impairment, particularly in proliferative form (proliferative DR [PDR]). The impact PDR microenvironment on microglia, which are resident immune cells central nervous system, and specific pathological changes it may induce remain unclear. This study aimed to investigate role microglia progression under hypoxic inflammatory conditions. We performed a comprehensive gene expression analysis using human-induced pluripotent stem...

Short-term outcomes in patients with branch retinal vein occlusion who received intravitreal aflibercept or without ranibizumab Yoshihito Sakanishi, Ayumi Usui-Ouchi, Kazunori Tamaki, Keitaro Mashimo, Rei Ito, Nobuyuki Ebihara Department of Ophthalmology, Juntendo University Urayasu Hospital, City, Chiba, Japan Purpose: The purpose this study was to determine the short-term for (IVA) (IVR) macular edema (ME) due (BRVO). Patients and methods: IVA ME BRVO. initially were defined as...

Numerous mast cells are present in the choroid, but effects of cell mediators on retinal pigment epithelial (RPE) not well understood. We investigated influence RPE vitro, focusing tryptase. Expression receptors was examined by reverse transcription polymerase chain reaction. also assessed production interleukin 8 and vascular endothelial growth factor (VEGF) after were stimulated with using an antibody array enzyme-linked immunosorbent assay. Furthermore, we tryptase migration integrity...

Vernal keratoconjunctivitis (VKC) is a severe, recurrent allergic conjunctivitis. Previously, we found high concentrations of oncostatin M (OSM) in the tears patients with VKC. Here, investigated role OSM VKC by focusing on epithelial barrier function and IL-33 production.To assess effect human conjunctival cells (HConEpiCs), measured transepithelial electrical resistance dextran permeability. We also assessed expression tight junction-related proteins such as E-cadherin ZO-1 HConEpiCs...

Purpose: The aim of the study is to explore effectiveness using monochrome mode with Brilliant Blue G during vitrectomy surgery NGENUITY 3D Visualization System. Methods: Vitrectomy cases involving BBG-stained internal limiting membrane peeling System (Alcon Laboratories, Inc.) at Juntendo University Urayasu Hospital from October 2022 February 2023 were selected. contrast ratios between peeled and stained areas under three settings assessed: normal, yellow, monochrome. In setting, a yellow...

Objectives: Ophthalmic three-dimensional (3D) digital surgery can reproduce high-definition surgical images; however, 3D is limited by recording capacities. We examined the relationship between minimum image quality required to images and capacity. Methods: Patients who underwent simultaneous vitrectomy cataract same surgeon using a system at Juntendo University Urayasu Hospital February October 2021 were evaluated. Various (Q) frame rate (FR) settings used for each case. Four vitreous...