A5008839354- Bacterial biofilms and quorum sensing
- Gut microbiota and health
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Cystic Fibrosis Research Advances
- Aquaculture disease management and microbiota
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Inhalation and Respiratory Drug Delivery
- Diet and metabolism studies
- Oral microbiology and periodontitis research
- Microbial Metabolic Engineering and Bioproduction
- Antimicrobial Peptides and Activities
- Salmonella and Campylobacter epidemiology
- Bacterial Identification and Susceptibility Testing
- Advanced biosensing and bioanalysis techniques
- Helicobacter pylori-related gastroenterology studies
- Influenza Virus Research Studies
- Mycobacterium research and diagnosis
- Bacillus and Francisella bacterial research
- Biosensors and Analytical Detection
- Escherichia coli research studies
Yonsei University
2015-2024
Korea Institute of Brain Science
2014-2021
Severance Hospital
2020-2021
Dankook University
2012-2020
Dinona (South Korea)
2010
Harvard University
2006-2009
University of Otago
2009
University of Cincinnati Medical Center
2002-2007
University of Cincinnati
2004
The University of Texas Health Science Center at San Antonio
2004
Francisella tularensis is able to survive and grow within macrophages, a trait that contributes pathogenesis. Several genes have been identified are important for intramacrophage survival, including mglA iglC. F. also amoebae. It shown here iglC mutant strains not only defective survival replication the macrophage-like cell line J774, but Acanthamoebae castellanii . Moreover, these highly attenuated virulence in mice, suggesting common mechanism underlies intraamoebae virulence. A 2D gel...
Mucoid, mucA mutant Pseudomonas aeruginosa cause chronic lung infections in cystic fibrosis (CF) patients and are refractory to phagocytosis antibiotics. Here we show that mucoid bacteria perish during anaerobic exposure 15 mM nitrite (NO2–) at pH 6.5, which mimics CF airway mucus. Killing required a lower than 7, implicating formation of nitrous acid (HNO2) NO, adds NO equivalents cellular molecules. Eighty-seven percent isolates possessed mutations were killed by HNO2 (3-log reduction 4...
Abstract To address the drug-resistance of bacterial pathogens without imposing a selective survival pressure, virulence and biofilms are highly attractive targets. Here, we show that terrein, which was isolated from Aspergillus terreus , reduced factors (elastase, pyocyanin, rhamnolipid) biofilm formation via antagonizing quorum sensing (QS) receptors affecting Pseudomonas aeruginosa cell growth. Additionally, effects terrein on production QS signaling molecules expression QS-related genes...
Pseudomonas aeruginosa displays intrinsic resistance to many antibiotics and known acquire actively genetic mutations for further resistance. In this study, we attempted understand genomic transcriptomic landscapes of P. clinical isolates that are highly resistant multiple antibiotics. We also aimed reveal a mode antibiotic by elucidating transcriptional response genes conferring To end, sequenced the whole genomes profiled genome-wide RNA transcripts three different multi-drug (MDR)...
Staphylococcus epidermidis is one of the most abundant colonizers healthy human mucosa including that in respiratory tract. As microbiome has been linked to host immune responses, this study sought determine role nasal mucosa-associated S. innate responses against influenza A virus (IAV). strains were isolated from mucus samples individuals. The effects these mucosa-derived commensal on interferon (IFN)-dependent immunity and IAV infection dynamics tested vitro using normal epithelial (NHNE)...
Pseudomonas aeruginosa, a gram-negative bacterium of clinical importance, forms more robust biofilm during anaerobic respiration, mode growth presumed to occur in abnormally thickened mucus layer lining the cystic fibrosis (CF) patient airway. However, molecular basis behind this anaerobiosis-triggered formation is not clearly defined yet. Here, we identified morphological change naturally accompanied by respiration P. aeruginosa and investigated its effect on vitro. A standard laboratory...
Abstract Pseudomonas aeruginosa establishes airway infections in Cystic Fibrosis patients. Here, we investigate the molecular interactions between P. and mucus secretions (AMS) derived from primary cultures of normal human tracheal epithelial (NHTE) cells. PAO1, a prototype strain , was capable proliferating during incubation with AMS, while all other tested bacterial species perished. A PAO1 mutant lacking PA4834 gene became susceptible to AMS treatment. The Δ grown supplemented 100 μM...
Abstract Background Recent evidence suggests that the commensal microbes act as a barrier against invading pathogens and enteric infections are consequences of multi-layered interactions among commensals, pathogens, host intestinal tissue. However, it remains unclear how perturbations gut microbiota compromise infection resistance, especially through changes at species metabolite levels. Results Here, we illustrate Bacteroides vulgatus , dominant Bacteroidetes phylum in mouse intestine,...
Metabolic dysfunction-associated steatotic liver disease (MASLD), previously known as Non-Alcoholic Fatty Liver Disease, is a widespread condition characterized by excessive fat buildup in hepatocytes without significant alcohol consumption. Manipulation of the gut microbiome has been considered to prevent and improve occurrence progression MASLD, particularly through gut-liver axis. This study aimed investigate correlation between function determine whether can ameliorate MASLD. We...
ABSTRACT Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses metabolically engineered E. accumulation poly(3-hydroxybutyrate) (PHB) in early stationary phase analyzed at protein level by two-dimensional gel electrophoresis. Out 20 proteins showing altered expression levels with PHB, 13 identified aid mass spectrometry. Three heat...
ABSTRACT Vibrio cholerae is an aquatic bacterium that causes the severe diarrheal disease cholera. V. strains of O1 serogroup exist as two biotypes, classical and El Tor. Toxigenic Tor biotype emerged to cause seventh pandemic cholera in 1961 subsequently displaced both environment a within decade. The factors drove emergence displacement are unknown. Here, we show unique difference carbohydrate metabolism between these biotypes. When grown with added carbohydrates, generated sharp decrease...
We isolated a new lytic Pseudomonas aeruginosa phage that requires type IV pili for infection. PA1Ø has broad bactericidal spectrum, covering Gram-positive and Gram-negative bacteria, can eradicate biofilm cells. may be developed as therapeutic agent biofilm-related mixed infections with P. Staphylococcus aureus.
To investigate the epidemiological traits of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by Asian Network for Surveillance Resistant Pathogens (ANSORP).A total 16 MPPA were from six countries in 2000 to 2009 ANSORP. The MBL gene was detected PCR amplification. genetic organization class 1 integron carrying cassette investigated mapping and sequencing. Southern blotting, repetitive sequence-based multilocus sequence typing (MLST) experiments...
Vibrio cholerae is a gram-negative bacterium that causes cholera. Although the pathogenesis caused by this deadly pathogen takes place in intestine, commonly thought to be anaerobic, anaerobiosis-induced virulence regulations are not fully elucidated. Anerobic growth of V. strain, N16961, was promoted when trimethylamine N-oxide (TMAO) used as an alternative electron acceptor. Strikingly, cholera toxin (CT) production markedly induced during anaerobic TMAO respiration. N16961 mutants unable...
Broccoli extract (BE) has numerous beneficial effects on human health including anticancer activity. Quorum sensing (QS), mediated by self-produced autoinducer (AI) molecules, is a key process for the production of virulence determinants in pathogenic bacteria. BE suppressed AI-2 synthesis and AI-2-mediated bacterial motility dose-dependent manner Escherichia coli O157:H7. In addition, expression ler gene that regulates AI-3 QS system was also diminished response to treatment with BE....
Pseudomonas aeruginosa, an opportunistic pathogen of clinical importance, causes chronic airway infections in patients with cystic fibrosis (CF). Current literature suggests that pockets reduced oxygen tension exist the CF mucus. However, virulence features this under such conditions are largely unknown. Cell-free supernatant standard laboratory P. aeruginosa strain PAO1 obtained from anaerobic culture, but not aerobic failed to kill A549 human epithelial cells. Further investigation...