Haifeng Li

ORCID: 0000-0003-2982-1243
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About
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Research Areas
  • Genomics and Phylogenetic Studies
  • Microbial Metabolites in Food Biotechnology
  • Food composition and properties
  • Microbial Community Ecology and Physiology
  • Fermentation and Sensory Analysis
  • Irrigation Practices and Water Management
  • Microbial Natural Products and Biosynthesis
  • Probiotics and Fermented Foods
  • Insect and Pesticide Research
  • Research in Cotton Cultivation
  • Engineering and Agricultural Innovations
  • Microbial Metabolic Engineering and Bioproduction
  • Selenium in Biological Systems
  • Fungal Biology and Applications
  • Bacteriophages and microbial interactions
  • Plant biochemistry and biosynthesis
  • Microbial infections and disease research
  • Enzyme Production and Characterization
  • Food Quality and Safety Studies
  • Water Treatment and Disinfection
  • Photosynthetic Processes and Mechanisms
  • Pesticide Residue Analysis and Safety
  • Biofuel production and bioconversion
  • Electrochemical sensors and biosensors
  • Plant Toxicity and Pharmacological Properties

Henan University of Technology
2016-2025

Guangdong Ocean University
2024

Key Laboratory of Guangdong Province
2024

Agricultural Product Processing Research Institute
2024

First Hospital of Jilin University
2024

Jilin University
2024

University of Shanghai for Science and Technology
2024

Beijing Academy of Agricultural and Forestry Sciences
2023-2024

Jiangxi Agricultural University
2019-2022

Xinjiang Institute of Ecology and Geography
2019

Geranylgeranyl diphosphate synthase (GGPPS) is the crucial bottleneck in carotenoid biosynthesis. However, low activity limits broad application of GGPPS. In this study, OsGGPPS1 rice was engineered based on ancestral sequence reconstruction (ASR) and semirational design to improve catalytic performances existing The better mutant A22R/A26P with improved enzyme generated ASR. Additionally, mutants as V162A/M218S/F227Y designed using a design. combinatorial assembly d-OsGGPPS1...

10.1021/acs.jafc.4c05029 article EN Journal of Agricultural and Food Chemistry 2024-08-13

Fatty acid ethyl esters (FAEE) are key target for wine industry to diversify its production towards fruity flavor. The microbial community and functional enzymes governing FAEE formation during Chinese rice (Huangjiu) brewing were investigated in current study. Physicochemical characteristics displayed distinct phased changes, which fluctuated sharply initial fermentation then leveling off gently. Except reducing sugar, other factors showed strong positive correlations with the dominant...

10.1016/j.lwt.2024.116035 article EN cc-by-nc-nd LWT 2024-04-01

Lotus roots (Nelumbo nucifera Gaertn.) are rich in nutrients and have ornamental food value. However, browning has caused huge economic losses security risks during the storage harvesting of fresh-cut lotus. This study investigated role melatonin inhibiting lotus browning, illustrates its molecular mechanism.The application effectively retarded process enhanced reactive oxygen species (ROS) scavenging enzyme activity, inhibited activity polyphenol oxidase (PPO), peroxidase (POD). Melatonin...

10.1002/jsfa.12619 article EN Journal of the Science of Food and Agriculture 2023-04-13

An alginate lyase encoding gene sagl from Flavobacterium sp. H63 was codon optimized and recombinantly expressed at high level in P.pastoris through cell-density fermentation. The highest yield of recombinant enzyme (rSAGL) yeast culture supernatant reached 226.4 μg/mL (915.5 U/mL). This the record expression so far. rSAGL confirmed as a partially glycosylated protein EndoH digestion. optimal reaction temperature pH this were 45 °C 7.5; 80 mM K+ ions could improve catalytic activity by 244%...

10.3390/md16050158 article EN cc-by Marine Drugs 2018-05-11

The effect of temperature fluctuations on the freshness shrimp in simulated trays was investigated by setting a freeze–thaw (F-T) cycle 12 h after freezing at −20 °C and thawing 1 under refrigeration. results showed that shrimp’s physicochemical properties deteriorated to different extents with increase F-T cycles. total colony count 6.07 lg CFU/g 21 cycles, volatile saline nitrogen content reached 30.36 mg/100 g, which exceeded edible standard. In addition, sensory quality textural...

10.3390/foods13020305 article EN cc-by Foods 2024-01-18

Selenium (Se) is an essential trace element that plays a critical role in human tissue formation, metabolism, and physiological functions. However, many individuals worldwide suffer from Se deficiency diseases. This study aims to evaluate the impact of Se-tolerant LF-17 agents exogenous Na2SeO3 application on growth, enzyme activity, metabolic characteristics rape seedlings. Treatment LF-3 (inoculation agent Na2SeO3, with soil concentration 5 mg/kg) led 38.62% increase plant height 116.7%...

10.1186/s12870-025-06480-3 article EN cc-by-nc-nd BMC Plant Biology 2025-04-04

Abstract In this study, a mixed strain ( MS ) starter culture was prepared by fed batch fermentation method and its effect on wheat dough steamed bread quality investigated. The had higher leavening ability more viable yeast counts than traditional TMS made static method. A slight decrease observed in the extensibility of fermented yeast, as resting time increased. leavened that but similar to during overall fermenting process. No significant differences could be between resistance extension...

10.1111/jtxs.12064 article EN Journal of Texture Studies 2014-02-21

MABR for effective treatment of domestic saline sewage and its microbial community.

10.1039/c7ra03755d article EN cc-by-nc RSC Advances 2017-01-01

During Streptococcus zooepidemicus fermentation, most carbon sources are used to synthesize lactic acid, which can inhibit strain growth and hyaluronic acid production. Here, we expressed bacterial haemoglobin (Vhb) in Strep. zooepidemicus. Due highly efficient oxygen use, only 15·26 g l−1 was produced, is 0·73 times the quantity produced by control strain. Compared with (1·61 l−1), (HA) production this did not substantially increase, 2·16 l−1. Next, a series of...

10.1111/lam.12546 article EN Letters in Applied Microbiology 2016-01-19

Agricultural soil contamination by pesticide residues has become a serious issue of increasing concern due to their high persistence and toxicity non-target species. However, as the world's largest peach producer, national scale surveys on in orchard soils are scarce China. In this study, target suspect screening method covering over 200 pesticides commonly used orchards was developed using ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry...

10.1016/j.ecoenv.2023.114664 article EN cc-by-nc-nd Ecotoxicology and Environmental Safety 2023-02-19

Corn straw is considered a renewable biomass energy source, and its unreasonable disposal leads to resource waste environmental pollution. Black soldier fly (Hermetia illucens L.) larvae (BSFL) facilitate the bioconversion of various types organic wastes. In this study, we found that 88% BSFL survived, 37.4% corn was digested after 14 days feeding with straw. Contrary expectations, pretreatment alkaline hydrogen peroxide did not promote digestion but rather reduced growth survival rates...

10.3390/insects15100734 article EN cc-by Insects 2024-09-24

A novel actinobacterial strain, 3-wff-81(T), was isolated from interfacial sediment of the eutrophic Taihu Lake in Jiangsu Province (China) and subjected to polyphasic taxonomic characterization. The strain formed pale orange-pigmented colonies comprising rod-shaped cells on R2A agar. Phylogenetic analysis based 16S rRNA gene sequences showed that 3-wff-81(T) belonged genus Geodermatophilus, with Geodermatophilus soli PB34(T) (99.1 % similarity) terrae PB261(T) (98.3 as closest relatives....

10.1099/ijs.0.049460-0 article EN INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 2013-06-08

This study investigated the effect of Lactobacillus plantarum DM616 on dough fermentation for making Chinese steamed bread. Dough was carried out via inoculation with Saccharomyces cerevisiae as monoculture, or in combination L. co-culture. The presence caused faster depletion reducing sugars and had little maximum specific growth rate (μmax) final cfu/g sourdough S. cerevisiae. In co-culture, pH varied from 3.2 to 3.5 total titratable acids developed 9.2 after 24 h fermentation. did not...

10.1111/jfpp.12205 article EN Journal of Food Processing and Preservation 2014-01-14

An aerobic, motile, Gram-stain-negative bacterium, designated strain NS1T, was isolated from interfacial sediment Taihu Lake, China. The formed yellow colonies on R2A medium. Cells were ovoid to rod-shaped and non-spore-forming. Growth occurred at 15-40 °C (optimum, 28 °C), pH 5.0-10.5 6.5-7.5) in the presence of 0-1 % (w/v) NaCl 0 %). Phylogenetic trees based 16S rRNA gene sequences showed that NS1T represented a member genus Altererythrobacter had highest sequence similarity troitsensis...

10.1099/ijsem.0.003299 article EN INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 2019-02-20
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