This paper demonstrates the first microbiological sampling of Outokumpu deep borehole (2516 m deep) aiming at characterizing bacterial community composition and diversity sulphate-reducing bacteria (SRB) in Finnish crystalline bedrock aquifers. Sampling was performed using a 1500-m-long pressure-tight tube that provided 15 subsamples, each corresponding to 100-m section down borehole. Microbial density measurements, as well fingerprinting with 16S rRNA gene-based denaturing gradient gel...

Abstract. The bacterial and archaeal community composition the possible carbon assimilation processes energy sources of microbial communities in oligotrophic, deep, crystalline bedrock fractures is yet to be resolved. In this study, intrinsic from groundwater six fracture zones 180 2300 m depths Outokumpu were characterized using high-throughput amplicon sequencing metagenomic prediction. Comamonadaceae-, Anaerobrancaceae- Pseudomonadaceae-related operational taxonomic units (OTUs) form core...

Active microbial communities of deep crystalline bedrock fracture water were investigated from seven different boreholes in Olkiluoto (Western Finland) using bacterial and archaeal 16S rRNA, dsr B, mcr A gene transcript targeted 454 pyrosequencing. Over a depth range 296–798 m below ground surface the changed according to depth, salinity gradient, sulphate methane concentrations. The highest diversity was observed sulphate-methane mixing zone (SMMZ) at 250–350 whereas lowest boundaries SMMZ....

Deep fracture zones in Finnish crystalline bedrock have been isolated for long, the oldest fluids being tens of millions years old. To accurately measure native microbial diversity fracture-zone fluids, water samples were obtained by isolating borehole fraction spanning a deep subsurface aquifer zone with inflatable packers (500 and 967 m) or pumping directly from zone. Sampling frequency was examined to establish time required space between be flushed replaced indigenous fluids. Chemical...

The long-term safety of final disposal spent nuclear fuel in the deep geosphere is dependent on stability biogeochemical conditions at site. Microbial processes, such as sulphate reduction and methanogenesis, may have profound effects site biogeochemistry. In this study, sulphate-reducing bacteria methane-producing archaea were investigated depths ranging from 68 to 545 m crystalline rock fractures an intended Olkiluoto, Finland. Denaturing gradient gel electrophoresis detected diverse...

Recent advances in sequencing technologies generate new predictions and hypotheses about the functional roles of environmental microorganisms. Yet, until we can test these at a scale that matches our ability to them, most them will remain as hypotheses. Function-based mining metagenomic libraries provide direct linkages between genes, metabolic traits microbial taxa thus bridge this gap sequence data generation predictions. Here developed high-throughput screening assays for function-based...

Two long-term potentially oil exposed Baltic Sea coastal sites near old refineries and harbours were compared to nearby less in terms of bacterial, archaeal fungal microbiomes degradation potential. The diversities similar sampling based on bacterial 16S rRNA gene 5.8S amplicon sequencing from both DNA RNA fractions. number genes participating alkane (alkB) or PAH-ring hydroxylation (PAH-RHDα) detected by qPCR all water sediment samples. These numbers correlated with the copies samples but...

Microorganisms in the deep biosphere are believed to conduct little metabolic activity due low nutrient availability these environments. However, destructive penetration long-isolated bedrock environments during construction of underground waste repositories can lead increased and potentially affect long-term stability repository systems, Here, we studied how microorganisms present fracture fluid from a depth 500 m Outokumpu, Finland, respond simple carbon compounds (C-1 compounds) presence...

Acetate plays a key role as electron donor and acceptor serves carbon source in oligotrophic deep subsurface. It can be produced from inorganic by acetogenic microbes or through breakdown of more complex organic matter. is an important molecule for sulfate reducers that are substantially present several bedrock environments. Aceticlastic methanogens use acetate and/or source. The goal this study was to shed light on cycling competition microbial communities fracture fluids Finnish...

Xylanases are in important class of industrial enzymes that essential for the complete hydrolysis lignocellulosic biomass into fermentable sugars. In present study, we report cloning novel xylanases with interesting properties from compost metagenomics libraries. Controlled composting materials was used to enrich microbial population lignocellulolytic organisms. DNA extracted samples construct libraries, which were screened xylanase activity. total, 40 clones exhibiting activity identified...

ABSTRACT Sulfate-reducing bacteria (SRB) participate in microbially induced corrosion (MIC) of equipment and H 2 S-driven reservoir souring oil field sites. Successful management industrial processes requires methods that allow robust monitoring microbial communities. This study investigated the applicability denaturing high-performance liquid chromatography (DHPLC) targeting dissimilatory sulfite reductase ß-subunit ( dsrB ) gene for SRB communities samples from North Sea, United States,...

The deep biosphere contains a large portion of the total microbial communities on Earth, but little is known about carbon sources that support life. In this study, we used Stable Isotope Probing (SIP) and high throughput amplicon sequencing to identify acetate assimilating at 2260 m depth in bedrock Outokumpu, Finland. long-term short-term effects were assessed by DNA-targeted SIP RNA targeted cell activation. reacted within hours amended acetate. Archaeal taxa representing rare identified...

A suite of molecular methods targeting 16S rRNA genes (i.e., DGGE, clone and high-throughput [HTP] amplicon library sequencing) was used to profile the microbial communities in deep Fennoscandian crystalline bedrock fracture fluids. Variation among bacterial examined with two commonly primer pairs: P1/P2 U968f/U1401r. DGGE using U968f/ U1401r mostly detected β-, γ-proteobacteria Firmicutes, while primers additionally other proteobacterial clades candidate divisions. However, combination...

This perspective reflects on the implementation of a multidisciplinary consortium project combining biological, chemical and computational sciences to discover develop new enzymes for carbon dioxide fixation.

Abstract Background Behavioral therapy has proved effective as rumination therapy. Our objective was to treat patients using multidisciplinary behavioral aimed at reducing ≥2 of the score. Methods All fulfilled Rome IV criteria for and were referred speech psychoeducation, diaphragmatic breathing exercises guided eating, physiotherapy relax thoracic abdominal muscles, consultation with psychologist dietitian. Symptoms, depression, anxiety, health‐related quality life (HRQoL), functional...

Abstract. The bacterial and archaeal community composition the possible carbon assimilation processes energy sources of microbial communities in oligotrophic, deep, crystalline bedrock fractures is yet to be resolved. In this study, intrinsic from six fracture zones 180–2300 m depths Outokumpu were characterized using high-throughput amplicon sequencing metagenomic prediction. Comamonadaceae-, Anaerobrancaceae- Pseudomonadaceae-related OTUs form core deep Outokumpu. Archaeal mainly composed...