A5056307599- Epigenetics and DNA Methylation
- Muscle metabolism and nutrition
- Genetics and Neurodevelopmental Disorders
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- Genetics and Physical Performance
- Cardiomyopathy and Myosin Studies
- Diet and metabolism studies
- Cardiac Fibrosis and Remodeling
- Genomics and Rare Diseases
- Cardiac Ischemia and Reperfusion
- Congenital heart defects research
- Neurogenetic and Muscular Disorders Research
- Genetic Associations and Epidemiology
- Mitochondrial Function and Pathology
- Exercise and Physiological Responses
- Cancer-related gene regulation
Victor Chang Cardiac Research Institute
2024-2025
Bond University
2018-2023
Abstract Background Ageing is associated with DNA methylation changes in all human tissues, and epigenetic markers can estimate chronological age based on patterns across tissues. However, the construction of original pan‐tissue clock did not include skeletal muscle samples hence exhibited a strong deviation between this tissue. Methods To address this, we developed more accurate, muscle‐specific genome‐wide data 682 from 12 independent datasets (18–89 years old, 22% women, 99% Caucasian),...
Skeletal muscle unloading due to joint immobilization induces atrophy, which has primarily been attributed reductions in protein synthesis humans. However, no study evaluated the skeletal proteome response limb using SWATH proteomic methods. This characterized shifts individual abundance and corresponding gene sets after 3 14 d of unilateral lower otherwise healthy young men. Eighteen male participants (25.4 ±5.5 y, 81.2 ±11.6 kg) underwent knee-brace with dietary provision following...
New Findings What is the central question of this study? The extent to which genetics determines adaptation endurance versus resistance exercise unclear. Previously, a divergent selective breeding rat model showed that genetic factors play major role in response aerobic training. Here, we asked: do underpin poor training affect functional overload? main finding and its importance? Our data show heritable low responders generated differential gene expression was associated with impaired...
Skeletal muscle atrophy is a physiological response to disuse, aging, and disease. We compared changes in mass the transcriptome profile after short-term immobilization divergent model of high low responders endurance training identify biological processes associated with early response. Female rats selectively bred for (HRT) (LRT; n = 6/group; generation 19) underwent 3 day hindlimb cast compare plantaris soleus muscles line-matched controls (n 6/group). RNA sequencing was utilized Gene...
Phenotypic and transcriptomic evidence of early cardiac aging, associated mechanisms, were investigated in young to middle-aged male mice (C57Bl/6; ages 8, 16, 32, 48 wks). Left ventricular gene expression (profiled via Illumina MouseWG-6 BeadChips), contractile coronary function, stress-resistance assessed Langendorff perfused hearts under normoxic conditions following ischemic insult (20 min global ischemia-45 reperfusion; I-R). Baseline or function was unaltered by age, while 'reserves'...
Abstract Knowledge of age-related DNA methylation changes in skeletal muscle is limited, yet this tissue severely affected by aging humans. Using a large-scale epigenome-wide association study (EWAS) meta-analysis age human from 10 studies (total n = 908 methylomes), we identified 9,986 differentially methylated regions at stringent false discovery rate < 0.005, spanning 8,748 unique genes, many which related to structure and development. We then integrated the results with known...
Abstract Background Ageing is associated with DNA methylation changes in all human tissues, and epigenetic markers can estimate chronological age based on patterns across tissues. However, the construction of original pan-tissue clock did not include skeletal muscle samples, hence exhibited a strong deviation between this tissue. Methods To address this, we developed more accurate, muscle-specific genome-wide data 682 samples from 12 independent datasets (18-89 years old, 22% women, 99%...
Abstract Exercise training prevents age-related decline in muscle function. Targeting epigenetic aging is a promising actionable mechanism and late-life exercise mitigates rodent muscle. Whether can decelerate, or reverse humans unknown. Here, we performed powerful meta-analysis of the methylome transcriptome an unprecedented number human skeletal samples (n = 3,176). We show that: 1) individuals with higher baseline aerobic fitness have younger transcriptomic profiles, 2) leads to...