A5015472152- Gene Regulatory Network Analysis
- Evolution and Genetic Dynamics
- Fungal and yeast genetics research
- Lattice Boltzmann Simulation Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanomaterials and Printing Technologies
- CRISPR and Genetic Engineering
- Fluid Dynamics and Heat Transfer
- thermodynamics and calorimetric analyses
- Bioinformatics and Genomic Networks
- Antimicrobial Peptides and Activities
- Microbial Metabolic Engineering and Bioproduction
- Microfluidic and Capillary Electrophoresis Applications
- Fluid Dynamics and Mixing
- Nanopore and Nanochannel Transport Studies
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Viral Infectious Diseases and Gene Expression in Insects
- Drug Solubulity and Delivery Systems
- Mathematical and Theoretical Epidemiology and Ecology Models
- Nanofabrication and Lithography Techniques
- Electrowetting and Microfluidic Technologies
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Pediatric health and respiratory diseases
Weizmann Institute of Science
2021-2025
Harvard University
2017-2020
Italian Institute of Technology
2019
Istituto per le Applicazioni del Calcolo Mauro Picone
2019
Harvard University Press
2017
Technion – Israel Institute of Technology
2006-2014
Bridge University
2011
The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely bacteria, and only once archaea. We show that uniaxial compression induces haloarchaea, forming tissue-like structures. These archaeal tissues are mechanically molecularly distinct from their unicellular lifestyle, mimicking several eukaryotic features. Archaeal undergo multinucleate stage followed by tubulin-independent cellularization, orchestrated active membrane tension at cell...
Step emulsification is an attractive method for production of monodisperse drops. Its main advantage the ability to parallelize many step emulsifier nozzles achieve high rates. However, sensitive any obstructions at nozzle exit. At rates, drops can accumulate exits, disturb formation subsequent and impair monodispersity. As a result, parallelized devices typically do not work maximum productivity. Here design introduced that parallelizes hundreds nozzles, effectively removes from exits. The...
Cells adjust their transcriptional state to accommodate environmental and genetic perturbations. An open question is what extent response perturbations has been specifically selected along evolution. To test the possibility that reprogramming does not need be 'pre-designed' lead an adaptive metabolic on physiological timescales, we confronted yeast cells with a novel challenge they had previously encountered. We rewired genome by recruiting essential gene, HIS3, from histidine biosynthesis...
The copy number of any protein fluctuates among cells in a population; characterizing and understanding these fluctuations is fundamental problem biophysics. We show here that distributions measured under broad range biological realizations collapse to single non-Gaussian curve scaling by the first two moments. Moreover, all experiments variance found depend quadratically on mean, showing degree freedom determines entire distribution. Our results imply do not reflect specific molecular or...
Three-dimensional, time-dependent direct simulations of step emulsification micro-devices highlight two essential mechanisms for droplet formation: first, the onset an adverse pressure gradient driving a back-flow continuous phase from external reservoir to micro-channel. Second, striction flowing jet which leads its subsequent rupture. It is also shown that such rupture delayed and eventually suppressed by increasing flow speed dispersed within channel, due stabilising effect dynamic...
Abstract The recruitment of a gene to foreign regulatory system is major evolutionary event that can lead novel phenotypes. However, the evolvability potential cells depends on their ability cope with challenges presented by recruitment. To study this ability, we combined synthetic continuous culture and online measurements metabolic dynamics over long timescales. HIS3 from histidine synthesis pathway was recruited GAL system, responsible for galactose utilization in yeast S. cerevisiae....
Antimicrobial peptides (AMPs) have the potential to treat multidrug-resistant bacterial infections. However, clinical application of AMPs is prevented by their toxicity and poor proteolytic stability. Here, a site-specific approach used generate new improve efficacy against pathogens while reducing toxicity. We modified generated series antimicrobial from leucine- lysine-rich peptide Amp1L (LKLLKKLLKKLLKLL) incorporation an isopeptide bond retaining peptide's size, sequence, charge,...
Despite their evolutionary significance, little is known about the adaptation dynamics of genomically rewired cells in evolution. We have confronted yeast carrying a regulatory circuit with severe and unforeseen challenge. The essential HIS3 gene from histidine biosynthesis pathway was placed under exclusive regulation galactose utilization system. Glucose containing medium strongly represses GAL genes including these are required to operate this gene. show here that although there were no...
Frequently during evolution, new phenotypes evolved due to novelty in gene regulation, such as that caused by genome rewiring. This has been demonstrated comparing common regulatory sequences among species and identifying single mutations are associated with phenotypes. However, while a mutation changes element, regulation is accomplished network involving multiple interactive elements. Therefore, better understand we have studied how contributed the adaptation of cells challenge. We created...
The phenotypic state of the cell is commonly thought to be determined by set expressed genes. However, given apparent complexity genetic networks, it remains open what processes stabilize a particular state. Moreover, not clear how unique mapping between vector genes and cell's To gain insight on these issues, we study here expression dynamics metabolically essential in twin populations. We show that two yeast populations derived from single steady-state mother population exhibiting similar...
Antimicrobial peptides (AMPs), which can be modified to kill a broad spectrum of microoganisms or specific microorganism, are considered as promising alternatives combat the rapidly widespread, resistant bacterial infections. However, there still several obstacles overcome. These include toxicity, stability, and ability interfere with immune response resistance. To overcome these challenges, we herein replaced regular peptide bonds isopeptide produce new AMPs based on well-known synthetic...
Neo-Darwinian evolution has presented a paradigm for population dynamics built on random mutations and selection with clear separation of time-scales between single-cell mutation rates the rate reproduction. Laboratory experiments evolving populations until now have concentrated fixation beneficial mutations. Following Darwinian paradigm, these probed at low temporal resolution dictated by rare mutations, ignoring intermediate phenotypes. Selection however, works phenotypes rather than...
Fully three-dimensional, time-dependent, direct simulations of the non-ideal Navier-Stokes equations for a two-component fluid shed light into mechanism which inhibits droplet breakup in step emulsifiers below critical threshold width-to-height (w/h) ratio microfluidic nozzle. Below w/h ∼ 2.6, provide evidence smooth topological transition from confined rectangular channel geometry to an isotropic (spherical) expansion downstream nozzle step. Above such threshold, inner outer space involves...
Integration of ionic permselective medium (e.g., nanochannels, membranes) within microfluidic channels has been shown to enable on-chip desalination, sample purification, bioparticle sorting, and biomolecule concentration for enhanced detection sensitivity. However, the ion-permselective mediums are generally fixed properties cannot be dynamically tuned. Here we study a device consisting an array individually addressable elastic membranes connected in series on top single channel that can...
The fundamental dynamics of the cell cycle, underlying growth and reproduction, were previously found to be robust under a wide range environmental internal perturbations. This property was commonly attributed its network structure, which enables coordinated interactions among hundreds proteins. Despite significant advances in deciphering components autonomous this network, understanding interfaces cycle with other major cellular processes is still lacking. To gain insight into these...
The modern evolutionary synthesis assumes that mutations occur at random, independently of the environment in which they confer an advantage. However, there are indications cells facing challenging conditions can adapt rapidly, utilizing processes beyond selection pre-existing genetic variation. Here, we show a strong regulatory challenge induce many independent yeast cells, absence general mutagenesis. Whole genome sequencing cell lineages reveals repertoire within single lineage arose only...
The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely bacteria, and - until now never archaea. We show that uniaxial compression induces haloarchaea, forming tissue-like structures. These archaeal tissues are mechanically molecularly distinct from their unicellular lifestyle, mimicking several eukaryotic features. Notably, undergo coenocytic stage followed by tubulin-independent cellularization, orchestrated active membrane tension at...
Many newly developed active pharmaceutical ingredients (APIs) are poorly soluble in water and thus have a dissolution-limited bioavailability. The bioavailability of Biopharmaceutical Classification System (BCS) class II APIs increases if they dissolve faster; this can be achieved by increasing their surface-to-volume ratio, for example, through formulation as submicron particles. In paper, we develop supersonic spray dryer that enables rapid synthesis submicron-sized at room temperature....
The copy number of any protein fluctuates among cells in a population; characterizing and understanding these fluctuations is fundamental problem biophysics. We show here that distributions measured under broad range biological realizations collapse to single non-Gaussian curve scaling by the first two moments. Moreover all experiments variance found depend quadratically on mean, showing degree freedom determines entire distribution. Our results imply do not reflect specific molecular or...