A5063727089- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- Galectins and Cancer Biology
- Mast cells and histamine
- melanin and skin pigmentation
- Protein Tyrosine Phosphatases
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Genetic and rare skin diseases.
- Chronic Myeloid Leukemia Treatments
- Glycosylation and Glycoproteins Research
- Cancer and Skin Lesions
- Chronic Lymphocytic Leukemia Research
- PI3K/AKT/mTOR signaling in cancer
- RNA Research and Splicing
- Oral Health Pathology and Treatment
- Polyomavirus and related diseases
- Microbial Natural Products and Biosynthesis
- Cancer-related Molecular Pathways
- Food Allergy and Anaphylaxis Research
- Monoclonal and Polyclonal Antibodies Research
- Hippo pathway signaling and YAP/TAZ
- RNA regulation and disease
University of Helsinki
1992-2021
Helsinki University Hospital
2003-2021
Yale University
2003
La Jolla Institute for Immunology
1994-1998
Proteins of the 14-3-3 family can associate with, and/or modulate activity of, several protooncogene and oncogene products and, thus, are implicated in regulation signaling pathways. We report that is associated with another important transducing enzyme, phosphatidylinositol 3-kinase (PI3-K). A recombinant fusion protein bound tyrosine-phosphorylated proteins from antigen receptor-stimulated T lymphocytes. PI3-K was identified by immunoblotting enzymatic assays as one 14-3-3-binding resting...
The production of 3‐phosphorylated inositol phospholipids is implicated in regulation cell growth and transformation. To explore the role these lipids T antigen receptor (TCR)/CD3‐induccd signaling, we have examined effects a specific phosphatidylinositol 3‐kinasce (Ptdlns3K) inhibitor, wortmannin, overexpression two PtdIns3K constructs on activation down‐stream effectors anti‐CD3 treated cells. We report that treatment cells with wortmannin blocked anti‐CD3‐induced mitogen‐activated kinase...
In cells expressing the oncogenic Bcr-Abl tyrosine kinase, regulatory p85 subunit of phosphatidylinositol 3-kinase is phosphorylated on residues. We report that this phosphorylation event readily catalyzed by Abl and Lck protein-tyrosine kinases in vitro, or a catalytically activated Lck-Y505F co-transfected COS cells, endogenous transfected Jurkat T upon triggering their cell antigen receptor. Using these systems, we have mapped major site to Tyr-688 C-terminal SH2 domain p85. Tyrosine...
Abstract: Tyrosine phosphorylation and dephosphorylation are implicated in the regulation of cell growth differentiation. A diverse identification key regulatory proteins by their content phosphotyrosine has been hampered very low level tyrosine phosphorylation. This is presumably caused relative preponderance phosphatase activity many cells. We report that treatment hematopoietic cells with phenylarsine oxide (PAO), a membrane‐permeable inhibitor, induced dramatic accumulation number...
Abstract Activation of resting T lymphocytes by ligands to the cell receptor (TcR)/CD3 complex is initiated phosphorylation a number key regulatory proteins on specific tyrosine residues. One such protein heterodimeric enzyme phosphatidylinositol‐3‐kinase (PI3K). We recently found that this also rapidly activated following TcR/CD3 triggering and immunoprecipitated PI3K was in vitro direct phosphorylation. Here we show TcR/CD3‐induced activation Jurkat leukemia cells depend presence p56 lck...
Activation of resting T lymphocytes by ligands to the complex cell antigen receptor (TCR) and CD3 is initiated a series critical tyrosine phosphorylation dephosphorylation events. Protein‐tyrosine kinases Syk, Src Csk families CD45 protein‐tyrosine phosphatase (PTPase) are known be involved in these early biochemical reactions. We have found that one two T‐cell‐expressed SH2‐domain‐containing PTPases, SHPTP2, rapidly phosphorylated on upon addition anti‐CD3 mAbs. This response was absent...
Merkel cell carcinoma (MCC) is a rare and aggressive neuroendocrine malignancy of the skin. The origin MCC thus far unknown proposed cells include cells, pro-/pre- or pre-B epithelial stem dermal cells. In this study, we aimed to shed further light on possibility that subset tumors arise from skin by examining expression hair follicle epidermal markers in normal human We also elucidate any correlation between these tumor polyomavirus (MCPyV) status other clinicopathological characteristics...
Abstract: Normal melanocytes require growth support provided by the adjacent basement membrane. In contrast, nevus cells and melanoma survive in dermis, vitro on a soft collagen gel. Transforming factor‐β1 (TGF‐β1) produced themselves induces apoptosis normal cultured gel, an effect that can be counteracted fibroblast factor‐2 (FGF‐2). The purpose of this study was to investigate mechanisms which FGF‐2 counteracts apoptotic signals from TGF‐β1 We report did not interfere with signal...
The precise immune mechanisms behind cow's milk allergy (CMA) are still unknown. Previously, the production of cytokines TNF‐α and IFN‐γ in T cells from children with CMA has been shown to be decreased, IL‐4 increased when compared healthy children. As these aberrations cytokine may associated disturbances cellular function, we investigated whether T‐cell signal transduction is abnormal CMA. For this purpose evaluated activation MAP kinase Erk2. Thirty‐nine infants were included study. Of...
Abstract Trichothiodystrophy is a group of multisystem neuroectodermal disorders with dysplastic hair as the cardinal symptom. We describe three patients from two Finnish families in whom whole‐exome sequencing revealed novel homozygous variant, c.26del, p.(Pro9Glnfs*144) MPLKIP ‐gene, confirming diagnosis non‐photosensitive trichothiodystrophy type 4 (TTD4). The variant was confirmed by Sanger and inherited unaffected carrier parents. This report adds to literature expanding genetic...