A5080787873- DNA Repair Mechanisms
- RNA Research and Splicing
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Genomics and Chromatin Dynamics
- Biochemical and Structural Characterization
- RNA modifications and cancer
- Antimicrobial Peptides and Activities
- Biological Activity of Diterpenoids and Biflavonoids
- Mitochondrial Function and Pathology
- DNA and Nucleic Acid Chemistry
- Mass Spectrometry Techniques and Applications
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Microtubule and mitosis dynamics
- Flavonoids in Medical Research
- Metabolomics and Mass Spectrometry Studies
- Plant Molecular Biology Research
- Silk-based biomaterials and applications
- Plant Genetic and Mutation Studies
- Cellular transport and secretion
- Protein Kinase Regulation and GTPase Signaling
- Genetic Mapping and Diversity in Plants and Animals
Slovak Academy of Sciences
2015-2024
Cancer Research Institute of the Slovak Academy of Sciences
2015-2024
Biomedical Research Center of the Slovak Academy of Sciences
2019-2024
Yahoo (United Kingdom)
2005
Contipro (Czechia)
2004
Institute of Molecular Biology of the Slovak Academy of Sciences
2003
Institute of Chemistry of the Slovak Academy of Sciences
2001
Proper meiotic chromosome segregation, essential for sexual reproduction, requires timely formation and removal of sister chromatid cohesion crossing-over between homologs. Early in meiosis cohesins hold sisters together also promote DNA double-strand breaks, obligate precursors to crossovers. Later, cohesin cleavage allows segregation. We show that fission yeast redundant casein kinase 1 homologs, Hhp1 Hhp2, previously shown regulate segregation via phosphorylation the Rec8 subunit, are...
The formation of RNA–DNA hybrids, referred to as R-loops, can promote genome instability and cancer development. Yet the mechanisms by which R-loops compromise are poorly understood. Here, we establish roles for evolutionarily conserved Nrl1 protein in pre-mRNA splicing regulation, R-loop suppression maintaining stability. nrl1Δ mutants exhibit endogenous DNA damage, sensitive exogenous have defects homologous recombination (HR) repair. Concomitantly, cells display significant changes gene...
Berberine is a bioactive isoquinoline alkaloid derived from many plants. Although berberine has been shown to inhibit growth and induce apoptosis of several tumor cell lines, its poor absorption moderate activity hamper full therapeutic potential. Here, we describe the synthesis series 9-O-substituted derivatives with improved antiproliferative apoptosis-inducing activities. An analysis novel by EPR spectroscopy confirmed their similar photosensitivity analogous behavior upon UVA irradiation...
Pre-mRNA splicing plays a key role in the regulation of gene expression. Recent discoveries suggest that defects pre-mRNA splicing, resulting from dysfunction certain factors, can impact expression genes crucial for genome surveillance mechanisms, including those involved cellular response to DNA damage. In this study, we analyzed how cells with non-functional spliceosome-associated Gpl1-Gih35-Wdr83 complex respond Additionally, investigated regulating factors damage repair. Our findings...
The spliceosome is a complex molecular machine assembled from many components, which catalyzes the removal of introns mRNA precursors. Our previous study revealed that Nrl1 (NRDE-2 like 1) protein associates with proteins and regulates pre-mRNA splicing homologous recombination-dependent R-loop formation in fission yeast Schizosaccharomyces pombe. Here, we identify associated factors Ntr1, Ntr2, Brr2 Gpl1, poorly characterized G-patch domain-containing required for efficient splicing. This...
Pre-mRNA splicing plays a fundamental role in securing protein diversity by generating multiple transcript isoforms from single gene. Recently, it has been shown that specific G-patch domain-containing proteins are critical cofactors involved the regulation of processes. In this study, using knock-out strategy, affinity purification and yeast-two-hybrid assay, we demonstrated spliceosome-associated Gpl1 fission yeast S. pombe mediates interactions between putative RNA helicase Gih35...
Studies of the human defensins have been hampered by lack a simple expression system allowing for rapid production functional peptide forms. Here, we describe Saccharomyces cerevisiae AH22 that meets condition. The 42 amino acid form beta-defensin-1 was expressed under control ADH1 promoter. optimum conditions were determined and stable maintenance pVT103L-hBD-1 chimeric vector in yeast population confirmed. Expressed hBD-1 secreted into medium (approximately 55 microg l(-1)) purified using...
The phosphorylation of proteins modulates various functions and plays an important role in the regulation cell signaling. In recent years, label-free quantitative (LFQ) phosphoproteomics has become a powerful tool to analyze within complex samples. Despite great progress, studies protein are still limited throughput, robustness, reproducibility, hampering analyses that involve multiple perturbations, such as those needed follow dynamics phosphoproteomes. To address these challenges, we...
Pre-mRNA splicing is a key process in the regulation of gene expression. In fission yeast Schizosaccharomyces pombe, Nrl1 regulates and expression several genes non-coding RNAs, also suppresses accumulation R-loops. Here, we report analysis interactions between selected RNA-processing proteins function by phosphorylation. Bacterial two-hybrid system (BACTH) assays revealed that N-terminal region important for interaction with ATP-dependent RNA helicase Mtl1 while C-terminal spliceosome...
The canonical role of cohesin is to mediate sister chromatid cohesion. In addition, plays important roles in processes such as DNA repair and regulation gene expression. Mounting evidence suggests that various post-translational modifications, including phosphorylation, acetylation sumoylation regulate functions. Our mass spectrometry analysis purified from Schizosaccharomyces pombe cells revealed the subunit Psm1 methylated on two evolutionarily conserved lysine residues, K536 K1200. We...
The evolutionarily conserved Swi5-Sfr1 complex plays an important role in homologous recombination, a process crucial for the maintenance of genomic integrity. Here, we purified Schizosaccharomyces pombe from meiotic cells and analyzed it by mass spectrometry. Our analysis revealed new phosphorylation sites on Swi5 Sfr1. We found that mutations prevent Sfr1 do not impair their function but swi5 sfr1 mutants encoding phosphomimetic aspartate at identified are only partially functional....
Many cellular processes require the activities of complex molecular machines composed several protein subunits. Insights into these systems can be gained by isolation complexes followed in vitro analyses determining identity, posttranslational modifications, and interactions among proteins. Here, we present a protocol for tandem affinity purification (TAP) from fission yeast Schizosaccharomyces pombe. The employs cells expressing C-terminally TAP-tagged proteins is suitable analysis purified...