A5014636840- Mesenchymal stem cell research
- Pancreatic function and diabetes
- Advanced Biosensing Techniques and Applications
- Renal and related cancers
- Clinical Nutrition and Gastroenterology
- Monoclonal and Polyclonal Antibodies Research
- Extracellular vesicles in disease
- Cannabis and Cannabinoid Research
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Pharmaceutical studies and practices
- DNA Repair Mechanisms
- Pancreatitis Pathology and Treatment
- Tissue Engineering and Regenerative Medicine
- Microbial Metabolic Engineering and Bioproduction
- Hematopoietic Stem Cell Transplantation
- Bacterial Genetics and Biotechnology
- Arsenic contamination and mitigation
- Biotin and Related Studies
- Trace Elements in Health
- Neonatal Respiratory Health Research
- Microscopic Colitis
- MicroRNA in disease regulation
- Renal cell carcinoma treatment
- Organ Transplantation Techniques and Outcomes
Stanford University
2022-2025
Stanford Medicine
2022-2024
Scripps Research Institute
2016-2018
Indian Institute of Science Bangalore
2009-2012
Heavy metals and metalloids such as cadmium [Cd(II)] arsenic [As(III)] are widespread environmental toxicants responsible for multiple adverse health effects in humans. However, the molecular mechanisms underlying metal-induced cytotoxicity carcinogenesis, well detoxification tolerance pathways, incompletely understood. Here, we use global fitness profiling by barcode sequencing to quantitatively survey Schizosaccharomyces pombe haploid deletome genes that confer of or arsenic. We identified...
In this study, we designed a nanoscale platform for sustained amino acid delivery to support transplanted pancreatic islets. The features mesoporous silica nanoparticles (MSNPs) loaded with glutamine (G), an essential required islet survival and function, coated polydopamine (PD). We investigated various PD concentrations (0.5–2 mg/mL) incubation times h) optimize G release, identifying that concentration of 0.5 mg/mL incubated h yielded the best results viability functionality ex vivo,...
Mesenchymal stem cells (MSCs) are gaining increasing prominence as an effective regenerative cellular therapy. However, ensuring consistent and reliable effects across clinical populations has proved to be challenging. In part, this can attributed heterogeneity in the intrinsic molecular signature of MSCs, which is dependent on their source origin. The present work uses integrated omics-based profiling, at different functional levels, compare anti-inflammatory, immunomodulatory, angiogenic...
Systemic steroid exposure, while useful for the treatment of acute flares in inflammatory bowel disease (IBD), is associated with an array side effects that are particularly significant children. Technical advancements have enabled locoregional intraarterial delivery directly into specific segments gastrointestinal tract, thereby maximizing tissue concentration limiting systemic exposure. We investigated feasibility administration a cohort nine pediatric patients who had IBD. This approach...
Different DNA-binding proteins have different interaction modes with DNA. Sequence-specific DNA-protein has been mostly associated regulatory processes inside a cell, and as such extensive studies made. Adequate data is also available on nonspecific interaction, an intermediate to protein's search for its cognate partner. Multidomain involving physical sequestering of DNA often implicated regulate gene expression indirectly. However, this type limited. One the binding mycobacterium proteins....
Mesenchymal stem cells (MSCs) are a promising therapy to potentially treat diabetes given their potent anti-inflammatory and immune-modulatory properties. While these regenerative have shown considerable promise in cell culture, clinical translation has been challenging. In part, this can be attributed not reaching the pancreas exert effects following conventional intravenous (IV) injection, with majority of being trapped lungs pulmonary first-pass effect. present study, we will therefore...
Abstract Background Kidneys are at risk from drug-induced toxicity, with a significant proportion of acute kidney injury (AKI) linked to medications, particularly cisplatin. Existing cytoprotective drugs for cisplatin-AKI carry side effects, prompting search better biological therapies. Mesenchymal Stem Cells (MSCs) under consideration given their regenerative properties, yet clinical application has not achieved full potential, mainly due variability in the source MSC tested. In addition,...
Hexavalent chromium [Cr(VI)] damages DNA and causes cancer, but it is unclear which damage responses (DDRs) most critically protect cells from chromate toxicity. Here, genome-wide quantitative functional profiling, DDR measurements genetic interaction assays in Schizosaccharomyces pombe reveal a toxicogenomic profile that closely resembles the cancer chemotherapeutic drug camptothecin (CPT), traps Topoisomerase 1 (Top1)-DNA covalent complex (Top1cc) at 3' end of single-stand breaks (SSBs),...
Sequence specific interaction between DNA and protein molecules has been a subject of active investigation for decades now. Here, we have chosen single promoter containing bacteriophage DeltaD(III) T7 Escherichia coli RNA polymerase followed their recognition at the air-water interface by using surface plasmon resonance (SPR) technique, where movement one reacting species is restricted way arraying them on an immobilized support. For Langmuir monolayer studies, used with histidine tag...
At the heart of understanding cellular processes lies our ability to explore specific nature communication between sequential information carrying biopolymers. However, data extracted from conventional solution phase studies may not reflect dynamics recognized partners as they occur in crowded milieu. We use principle immobilization histidine-tagged biopolymers at a Ni(II)-encoded Langmuir monolayer study sequence-specific protein-protein interactions an artificially environment. The...
Graft versus host disease (GVHD) is one of the most serious complications following stem cell transplant in children and a major cause morbidity mortality. Corticosteroids remain mainstay treatment, although majority respond to systemic steroids, those refractory or dependent upon corticosteroids suffer from secondary long-term steroid administration. This problem has prompted consideration steroid-sparing treatment strategies, time clinical remission can be variable. Intraarterial...
In the present body of work, we investigated role pulsed focused ultrasound (pFUS) in modulating acutely injured kidney order to facilitate tissue regeneration using mesenchymal stem cells. Our initial data shows that pFUS is able modulate microenvironment, and can help regenerative capacity cells, especially when these cells are given intra-arterially into kidney.