Sirtuin 1 (Sirt1) is an essential modulator of cellular metabolism and has pleiotropic effects. It was recently reported that Sirt1 overexpression in kidney tubule ameliorates cisplatin-induced acute injury (AKI). However, whether pharmacological activation also a beneficial effect against the disease remains unclear. In this study, we aimed to evaluate SRT1720, potent specific activator Sirt1, could ameliorate AKI. We found SRT1720 treatment ameliorated renal failure histopathological...

Liver fibrosis is characterized by changes in tissue architecture and extracellular matrix composition. affects not only hepatocytes but also the non-parenchymal cells such as hepatic stellate (HSCs), which are essential for maintaining an intact liver structure function. Transforming growth factor β1 (TGF-β1) a multifunctional cytokine that induces through activation of Smad signaling pathways. To improve new therapeutic approach, synthetic TGF-β1/Smad oligodeoxynucleotide (ODN) was used to...

Periodontitis is a chronic inflammatory disease that contributes to the destruction of gingiva. Porphyromonas gingivalis (P. gingivalis) can cause periodontitis via its pathogenic lipopolysaccharides (LPS). Melittin, major component bee venom, known have anti-inflammatory and antibacterial effects. However, role melittin in response has not been elucidated periodontitis-like human keratinocytes. Therefore, we investigated effects on P. LPS (PgLPS)-treated HaCaT keratinocyte cell line. The...

Acute kidney injury (AKI) is a dose-limiting side effect of cisplatin therapy in cancer patients. However, effective therapies for cisplatin-induced AKI are not available. Oxidative stress, tubular cell death, and inflammation known to be the major pathological processes disease. 6-Shogaol component ginger exhibits anti-oxidative anti-inflammatory effects. Accumulating evidence suggest that 6-shogaol may serve as potential therapeutic agent various inflammatory diseases. whether exerts...

Abstract Kidney fibrosis is a common process of various kidney diseases leading to end‐stage renal failure irrespective etiology. Myofibroblasts are crucial mediators in through production extracellular matrix (ECM), but their origin has not been clearly identified. Many study proposed that epithelial and endothelial cells become myofibroblasts by dedifferentiation endothelial‐mesenchymal transition (EndoMT). TGF‐β1/Smad signaling plays role partly epithelial‐mensencymal (EMT) EndoMT. Thus,...

Atopic dermatitis (AD) is a chronic skin inflammatory disease characterized by recurrent eczema and itching. It caused poorly controlled immune response damage to the barrier. Purified bee venom (BV) natural toxin produced honeybees (Apis mellifera L.), well known for its anti‑inflammatory, analgesic anti‑cancer effects against various types of disease. However, treatment strategies based on anti‑inflammatory properties have not been adequately studied in AD. Thus, present study examined...

Atherosclerosis is a progressive chronic inflammatory condition that the cause of most cardiovascular and cerebrovascular diseases. The transcription factor nuclear factor‑κB (NF‑κB) regulates number genes involved in responses cells are critical to atherogenesis, signal transducer activator (STAT)3 key immunity inflammation. Decoy oligodeoxynucleotides (ODNs) bind sequence‑specific factors limit gene expression by interfering with vitro vivo. present study aimed investigate beneficial...

Periodontitis is a chronic inflammatory disease that leads to destruction of tooth supporting tissues. Porphyromonas gingivalis (P. gingivalis), especially its lipopolysaccharides (LPS), one major pathogens cause periodontitis. Bee venom (BV) has been widely used as traditional medicine for various diseases. Previous studies have demonstrated the anti-inflammatory, anti-bacterial effects BV. However, direct role and cellular mechanism BV on periodontitis-like human keratinocytes not...

Hyperlipidemia is a chronic disorder that plays an important role in the development of cardiovascular diseases, type II diabetes, atherosclerosis, hypertension, and non-alcoholic fatty liver disease. Hyperlipidemias have created worldwide health crisis impose substantial burden not only on personal but also societies economies. Transcription factors sterol regulatory element binding protein (SREBP) family are key regulators lipogenic genes liver. SREBPs regulate lipid homeostasis by...

Acne vulgaris is the most common disease of pilosebaceous unit. The pathogenesis this complex, involving increased sebum production and perifollicular inflammation. Understanding factors that regulate important in identifying novel therapeutic targets for treatment acne. Bee Venom (BV) melittin have multiple effects including antibacterial, antiviral, anti-inflammatory activities various cell types. However, anti-lipogenic mechanisms BV not been elucidated. We investigated models...

The use of cisplatin, a chemotherapy drug, is often limited due to its renal side effects such as acute kidney injury (AKI). However, there are no validated medications prevent or treat cisplatin-induced AKI. Oridonin the major bioactive component Isodon rubescens (Rabdosia rubescens) and exhibits anticancer, antioxidative, anti-inflammatory effects. Recent studies have shown that oridonin alleviated variety inflammatory diseases, including in rodents. This study was aimed at investigating...

Streptococcus pyogenes (S. pyogenes) bacteria cause almost all primary skin infections in humans. Bee venom (BV) and melittin (Mel) have multiple effects, including antibacterial anti-inflammatory activities. This study aims to demonstrate their effects on bacterial mouse infection using S. pyogenes. The dorsal was tape-stripped, then topically applied. BV or Mel were applied the lesion. tissues stained with hematoxylin eosin, while immunohistochemical staining performed anti-neutrophil....

Atopic dermatitis (AD) is an inflammatory skin disease characterized by intense pruritus and relapsable eczematous lesions. The hallmarks of AD are defects in the epidermal barrier immunoglobulin E (IgE)-mediated sensitization to several environmental allergens, as well immune disorder mediated imbalance toward T-helper-2 response. Melittin, a major component bee venom, has been studied various diseases. However, beneficial effects melittin on mouse with AD-like symptoms have not explored....

Atopic dermatitis (AD) is a common inflammatory skin disease caused by an immune disorder. Mast cells are known to be activated and granulated maintain allergic reaction, including rhinitis, asthma, AD. Although hypoxia-inducible factor-1 alpha (HIF-1α) signal transducer activator of transcription 5 (STAT5) play crucial roles in mast cell survival granulation, their effects need clarified disorders. Thus, we designed decoy oligodeoxynucleotide (ODN) synthetic DNA, without open ends,...

Fibrosis is a common pathological feature in most kinds of chronic kidney disease. Transforming growth factor β1 (TGF-β1) signaling the master pathway regulating fibrosis pathogenesis, which mothers against decapentaplegic homolog 3 (SMAD3) with signal transducer and activator transcription (STAT3) act as integrator various pro-fibrosis signals. We examine effects pomolic acid (PA) on mice unilateral ureteral obstruction (UUO) TGF-β1 stimulated fibroblast cells. UUO were observed severe...

Renal fibrosis is a common process of various kidney diseases. Autophagy an important cell biology to maintain cellular homeostasis. In addition, autophagy involved in the pathogenesis renal disease, including acute injury, glomerular diseases, and fibrosis. However, functional role remains poorly unclear. The mammalian target rapamycin (mTOR) plays negative regulatory autophagy. Signal transducer activator transcription 3 (STAT3) intracellular signaling that may regulate variety...

Autophagy in the proximal tubules may promote fibrosis by activating tubular cell death, interstitial inflammation, and production of pro-fibrotic factors. The signal transducer activator transcription 3 (STAT3) is activated as a potential factor, which mediates stimulation renal fibrosis. We investigated role STAT3 autophagy its effect on prevention In this study, we use synthesized decoy oligonucleotides (ODN), were injected into tail veins unilateral ureteral obstruction (UUO) mice, to...

Equilibrium tie-line data were determined for a fluorous biphasic systems at 298.15 K. Two phase mixtures used, consisting of perfluorinated solvent using mixture two fluorinated solvents (methyl nonafluorobutyl ether and perfluorohexane) as well an organic in which the studied toluene, 1,4-dioxane, dimethylformamide, respectively. Nonrandom liquid models applied to four ternary produced interaction parameters that well-correlated with equilibrium composition. These enabled prediction...

Acne vulgaris has a pathogenesis that involves increased sebum production and perifollicular inflammation. Sterol regulatory element-binding protein-1 (SREBP-1) peroxisome proliferator activated receptor-γ (PPAR-γ) are transcription factors regulate numerous genes involved in lipid biosynthesis. To improve new therapeutic approach, we designed the SREBP/PPAR decoy oligodeoxynucleotide (ODN), synthetic short DNA containing complementary sequences for SREBP PPAR factors. We aim to investigate...

Hepatic fibrosis is the wound-healing process of chronic hepatic disease that leads to end-stage hepatocellular carcinoma and demolition structures. Epithelial⁻mesenchymal transition (EMT) has been identified phenotypic conversion epithelium mesenchymal phenotype occurred during fibrosis. Smad decoy oligodeoxynucleotide (ODN) a synthetic DNA fragment containing complementary sequence transcription factor. Thus, this study evaluated antifibrotic effects ODN on carbon tetrachloride...

We formulate well-posed continuous-time generative flows for learning distributions that are supported on low-dimensional manifolds through Wasserstein proximal regularizations of $f$-divergences. Wasserstein-1 operators regularize $f$-divergences so singular can be compared. Meanwhile, Wasserstein-2 the paths by adding an optimal transport cost, i.e., a kinetic energy penalization. Via mean-field game theory, we show combination two proximals is critical formulating flows. Generative...