A5102896567- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Marine Toxins and Detection Methods
- Genomics and Phylogenetic Studies
- Microtubule and mitosis dynamics
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Microbial metabolism and enzyme function
- Microbial Metabolites in Food Biotechnology
- Biocrusts and Microbial Ecology
- Physiological and biochemical adaptations
- Planarian Biology and Electrostimulation
- Microbial Metabolic Engineering and Bioproduction
- Light effects on plants
- Hormonal Regulation and Hypertension
- Service-Oriented Architecture and Web Services
- Genetic and Kidney Cyst Diseases
- DNA Repair Mechanisms
- Vibrio bacteria research studies
- Animal Genetics and Reproduction
- Drug-Induced Ocular Toxicity
- Polar Research and Ecology
- Pharmacological Receptor Mechanisms and Effects
Yamaguchi University
2005-2024
Centre de Génétique Moléculaire
2012
Centre National de la Recherche Scientifique
2012
University of Tsukuba
1994-1995
ABSTRACT. The bacterium Holospora oblusa is a macronuclear‐specific symbiont of the ciliate Paramecium caudatum. H. obtusa ‐bearing paramecia could survive even after cells were quickly heated from 25°C to 35°C. To determine whether infection with confers heat shock resistance on its host, we isolated genes homologous protein hsp60 and hsp70 P. caudatum . deduced amino acid sequences both cDNAs highly hsp family other eukaryotes. Competitive PCR showed that ‐free expressed only trace amounts...
ABSTRACT. The bacterium Holospora is an endonuclear symbiont of the ciliate Paramecium . Previously, we reported that paramecia bearing macronuclear‐specific obtusa survived better than symbiont‐free paramecia, even under high temperatures unsuitable for growth. with symbionts expressed levels hsp70 mRNAs at 25 °C, a usual growth temperature. We report herein micronuclear‐specific elegans also acquire heat‐shock resistance. Even after removal bacteria from hosts by treatment penicillin,...
ABSTRACT Centrioles, cilia, and flagella are ancestral conserved organelles of eukaryotic cells. Among the proteins identified in proteomics ciliary Paramecium , we focus here on a protein, Bug22p, previously detected by cilia basal-body high-throughput studies but never analyzed per se . Remarkably, this protein is also present plants, which lack centrioles cilia. Bug22p sequence alignments revealed consensus positions that distinguish species with centrioles/cilia from plants. In antibody...
ABSTRACT The individual role of the outer dynein arm light chains in molecular mechanisms ciliary movements response to second messengers, such as Ca 2+ and cyclic nucleotides, is unclear. We examined gene termed chain 1 (LC1) Paramecium tetraurelia ( ODAL1 ), a homologue LC1 Chlamydomonas reinhardtii , by RNA interference (RNAi) using feeding method. -silenced -RNAi) cells swam slowly, their swimming velocity did not increase membrane-hyperpolarizing stimuli. Ciliary on cortical sheets...
Little is known about the phylogeny of family Vorticellidae at generic level because few comprehensive analyses molecular phylogenetic relationships between members this group have, so far, been done. As a result, positions some genera that were based originally on morphological remain controversial. In present study, we performed vorticellids sequence small-subunit (SSU) rRNA gene, including one species genus Apocarchesium , for which no has previously reported. Phylogenetic trees...
We identified six genes of Paramecium caudatum, which differentially expressed in Holospora obtusa-bearing and H. obtusa-free cells using differential display reverse transcribed PCR (DDRT-PCR). Northern blot analyses revealed that two the genes, CA10-3 CA20-2, were extensively cell, while other four, AS16-1, CS14, CS21 CA17-1, more cell. Putative amino acid sequences CA10-3, AS16-1 CA17-1 showed high homologies with known related to intracellular signaling, transcription aerobic metabolism....
The UV-B radiation contained in solar has deleterious effects on plant growth, development and physiology. Specific damage to DNA caused by UV involves the cyclobutyl pyrimidine dimers (CPD) (6-4) pyrimidone photoproducts. CPDs are repaired CPD photolyase via a UV-A/blue light-dependent mechanism. gene for class II been cloned from higher plants such as Arabidopsis, cucumbers rice. We isolated characterized cDNA genomic clone encoding spinach photolyase. consisted of 3777 bases 9 exons....
In a ciliate Paramecium, the presence of water channels on membrane contractile vacuole has long been predicted by both morphological and physiological data, however, to date either biochemical or molecular biological data have not provided. present study, examine aquaporin in we carried out RT-PCR with degenerated primers designed based ParameciumDB, an cDNA (aquaporin 1, aqp1) full-length ORF encoding 251 amino acids was obtained from Paramecium multimicronucleatum using RACE. The deduced...
Paramecium shows rapid forward swimming due to increased beat frequency of cilia in normal (forward swimming) direction response various kinds stimuli applied the cell surface that cause K+ -outflow accompanied by a membrane hyperpolarization. Some adenylate cyclases are known be functional channels membrane. Using gene-specific knockdown methods, we examined nine paralogues P. tetraurelia ascertain whether and how they involved mechanical stimulus-induced hyperpolarization-coupled...
Summary According to the classical genetic analysis in Paramecium caudatum by Tsukii & Hiwatashi (1983), E mating type of each syngen is expressed when cell bears alleles specific for at Mt locus. The O cells are homozygous null allele, mt , In such mt/mt specificity determined two other loci called MA and MB . Inthe study reported here, macronucleoplasmic transplantation technique was used test above hypothesis. When macronucleoplasm 3 (mating 3) injected into a macronucleus 12 12),...
In Paramecium, a mechanical stimulus applied to the posterior portion of cell causes transient increase in membrane permeability potassium ions, transiently rendering hyperpolarized state. Hyperpolarization Cyclic adenosine monophosphate (cAMP) concentration cilia, resulting fast-forward swimming cell. Schultz and coworkers (1992) reported that unique adenylate cyclase (AC)-coupled channel is involved reaction underlying this response, which known as “escape response.” However, AC...
Paramecium exhibits responsive behavior to environmental changes, moving either closer or further away from stimuli. Electrophysiological experiments have revealed that these behavioral responses are controlled by membrane potentials. Anoctamin, a Ca
Abstract When mechanical stimulation was applied to free swimming Paramecium , forward velocity transiently increased due activation of the posterior mechanosensory channels. The behavior response, known as “escape response,” requires membrane hyperpolarization and K‐channel type adenylate cyclases. Our hypothesis is that this escape response also involves hyperpolarization‐activated cyclic nucleotide‐gated (HCN) HCN channels are activated by modulated nucleotides such cAMP cGMP. They play a...
To restore wild type phenotypes in mutants controlled by recessive genes, microinjection of whole genomic DNA into recipient macronucleus Paramecium caudatum was performed. Donor carrying three wild-type genetic markers (exocytosis, behavior and mating type) introduced a cell which triple homozygote for the corresponding genes. Of phenotypes, trichocyst non-discharge mutant TND converted to not only total but also fractionated restriction fragments, although restored phenotype complete. This...