A5020938792- Particle Accelerators and Free-Electron Lasers
- Advanced X-ray Imaging Techniques
- Crystallography and Radiation Phenomena
- Connexins and lens biology
- Particle accelerators and beam dynamics
- Heme Oxygenase-1 and Carbon Monoxide
- Atomic and Subatomic Physics Research
- Particle Detector Development and Performance
- Nicotinic Acetylcholine Receptors Study
- Radiation Detection and Scintillator Technologies
- Magnetic confinement fusion research
- Diamond and Carbon-based Materials Research
- X-ray Spectroscopy and Fluorescence Analysis
- Advanced Surface Polishing Techniques
- High-pressure geophysics and materials
- Medical Imaging Techniques and Applications
- Gyrotron and Vacuum Electronics Research
- Muon and positron interactions and applications
- Neutrino Physics Research
- Barrier Structure and Function Studies
- Heat shock proteins research
- Advanced X-ray and CT Imaging
- Plasma Diagnostics and Applications
- Redox biology and oxidative stress
- Superconducting Materials and Applications
Stanford University
2022-2025
SLAC National Accelerator Laboratory
2020-2024
Menlo School
2022
Lehigh University
2017-2018
The University of Queensland
2017
Moravian University
2017
To investigate whether connexin phosphorylation regulates the known role of zonula occludens-1 protein (ZO-1) in gap junction (GJ) function, we generated and analyzed a series phosphomimetic phosphorylation-dead mutants by mutating conserved regulatory serine (S) residues 255, 279/282, 365, 368, 373 located C-terminal domain connexin43 (Cx43) into glutamic acid (E) or alanine (A) residues. All were translated stable, full-length proteins assembled GJs when expressed HeLa Madin-Darby canine...
We present an x-ray regenerative amplifier free-electron laser design capable of producing fully coherent hard pulses across a broad tuning range at high steady state repetition rate. The scheme leverages strong undulator taper and apertured diamond output-coupling cavity crystal to produce both peak average spectral brightness radiation that is 2 3 orders magnitude greater than conventional single-pass self-amplified spontaneous emission amplifiers. Refractive guiding in the postsaturation...
In this work, we describe the instrumentation used to perform first operando transmission X-ray microscopy (TXM) and simultaneous diffraction of laser melting simulating powder bed fusion on XCS instrument at Linac Coherent Light Source (LCLS) free-electron (XFEL). Our TXM with 40× magnification in regime 11 keV gave spatial resolutions down 940 nm per line pair, effective pixel sizes 206 nm, image integration times <100 fs, frame rates tunable between 2.1 119 ns for two probe frames (0.48...
Gap junctions (GJs) assembled from connexin (Cx) proteins allow direct cell-cell communication. While phosphorylation is known to regulate multiple GJ functions, much less about the role of ubiquitin in these processes. Using ubiquitylation-type-specific antibodies and Cx43 lysine-to-arginine mutants we show that ∼8% a GJ, localized central plaque domains, K63-polyubiquitylated on K264 K303. Levels localization ubiquitylation correlated well with: (1) short turnover rate Cxs GJs; (2) removal...
Stanford Synchrotron Radiation Lightsource serves a wide scientific community with its variety of X-ray capabilities. Recently, wiggler source located at beamline 10-2 has been employed to perform high-resolution rocking curve imaging (RCI) diamond and silicon crystals. RCI is invaluable for the development upcoming cavity-based sources SLAC, including free-electron laser oscillator. In this paper, apparatus described experimental results are provided validate design. Future improvements...
The incorporation of boron into a diamond lattice holds the potential to advance X-ray optics, offering capability manipulate various parameters lattice. This includes enhancing near-infrared absorption relative pure diamond, thereby enabling Q-switchable optics. use MeV implantation emerges as promising method for precisely doping However, these optics function effectively Bragg-reflecting mirrors, ion must be executed with meticulous attention maintaining strain-free, perfect study aimed...
Abstract We present an experimental demonstration of a stable, low-loss, large x-ray cavity operating at 1.2 Angstrom wavelength. The consists 4 high reflectivity single crystal diamond Bragg mirrors arranged in rectangular configuration with design round trip distance 14.2 m. Femtosecond laser pulses from free electron were coupled into the via transmission phase grating. efficiency and lifetime characterized quantitatively, indicating close to theoretical optical performance. By...
Electrons in an x-ray free electron laser (XFEL) develop periodic density fluctuations, known as microbunches, which enable the exponential gain of power XFEL. When beam microbunched at a hard wavelength is kicked, microbunches are often washed out due to dispersion and <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" display="inline"><a:msub><a:mi>R</a:mi><a:mn>56</a:mn></a:msub></a:math> bend. An achromatic (dispersion-free) bend with small <c:math...
The success of x-ray free-electron lasers (XFELs) in recent years has greatly advanced many scientific fields. However, most these XFELs suffer from a low longitudinal coherence and instabilities due to the stochastic start-up process. A cavity-based laser (CBXFEL) is possible future direction development fully coherent stable sources. One challenges CBXFEL requirement for <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" display="inline"><a:mrow><a:mi mathvariant="normal">μ</a:mi><a:mi...
ABSTRACT Gap junctions (GJs) assembled from connexin (Cx) proteins play a pivotal role in cell-to-cell communication by forming channels that connect the cytosols of adjacent cells. Connexin 43, best-studied Cx, is ubiquitously expressed vertebrates. While phosphorylation known to regulate multiple aspects GJ function, much less about ubiquitination plays these processes. Here we show using ubiquitination-type specific antibodies and Cx43 lysine (K) arginine (R) mutants portion GJs can...