A5046619240- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Protein purification and stability
- Immunotherapy and Immune Responses
- Blood groups and transfusion
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Virus-based gene therapy research
- T-cell and Retrovirus Studies
- Immune Response and Inflammation
- Oral and gingival health research
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Oral microbiology and periodontitis research
- Advanced Biosensing Techniques and Applications
- Cervical Cancer and HPV Research
- HIV Research and Treatment
- Influenza Virus Research Studies
- Peptidase Inhibition and Analysis
- Xenotransplantation and immune response
- Distributed Control Multi-Agent Systems
Tokushima University
2021-2025
OP Bio Factory (Japan)
2022
University of the Ryukyus
2014-2021
University of the Ryukyus University Hospital
2019
Tokyo University of Science
2006-2015
Dana-Farber Cancer Institute
2001-2014
Harvard University
2003-2014
Institute for Biological Sciences
2007
Brigham and Women's Hospital
2004
National Center for Infectious Diseases
2004
Effective prophylaxis and antiviral therapies are urgently needed in the event of reemergence highly contagious often fatal severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) infection. We have identified eight recombinant human single-chain variable region fragments (scFvs) against S1 domain spike (S) protein SARS-CoV from two nonimmune antibody libraries. One scFv 80R efficiently neutralized inhibited syncytia formation between cells expressing S those receptor...
CD26 is a T cell activation antigen that contains dipeptidyl peptidase IV activity and known to bind adenosine deaminase. The mechanism by which costimulation potentiates receptor-mediated activation, leading subsequent exertion of effector function, still not clearly defined. In this article, we demonstrate localizes into lipid rafts, targeting rafts necessary for signaling events through CD26. Importantly, aggregation anti-CD26 mAb crosslinking also causes coaggregation CD45 rafts....
ABSTRACT In this report, the antiviral activity of 80R immunoglobulin G1 (IgG1), a human monoclonal antibody against severe acute respiratory syndrome coronavirus (SARS-CoV) spike (S) protein that acts as viral entry inhibitor in vitro, was investigated vivo mouse model. When IgG1 given prophylactically to mice at doses therapeutically achievable humans, replication reduced by more than 4 orders magnitude below assay limits. The essential core region S required for binding identified...
West Nile virus has spread rapidly across the United States, and there is currently no approved human vaccine or therapy to prevent treat disease. Passive immunization with antibodies against envelope protein represents a promising means provide short-term prophylaxis treatment for infection. In this study, we identified panel of 11 unique single-chain variable region antibody fragments (scFvs) that bind virus. Selected scFvs were converted Fc fusion proteins (scFv-Fcs) tested in mice their...
Phylogenetic analyses have provided strong evidence that amino acid changes in spike (S) protein of animal and human SARS coronaviruses (SARS-CoVs) during between two zoonotic transfers (2002/03 2003/04) are the result positive selection. While several studies support some viruses inter-species adaptation, role neutralizing antibodies (nAbs) driving SARS-CoV evolution, particularly intra-species transmission, is unknown. A detailed examination infected convalescent sera could provide nAb...
Porphyromonas gingivalis (Pg) is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (OMVs) that contain lipopolysaccharide (LPS), gingipains, pathogen-derived DNA RNA. Pg-OMVs are involved in the pathogenesis of periodontitis. Pg-OMV-activated pathways induce production pro-inflammatory cytokines, interleukin (IL)-6, IL-8 human gingival epithelial cell line, OBA-9, were investigated. The role mitogen-activated protein kinase (MAPK) nuclear factor...
Abstract Aims To prevent peri-implantitis, we investigated the adhesion of periodontopathogenic bacteria to titanium surfaces using a hydrophobic 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer inhibit adhesion. Method and results We immersed plates (TiPs) coated with MPC in bacterial suspension for 30 min or 24 h measured number adherent bacteria. Bacteria adhering TiPs were observed scanning electron microscopy (SEM). Furthermore, mimicking an oral cavity, saliva, suspensions...
This study was to characterize and determine the classification of novel strain TM49T, isolated from a blood culture patient who developed bacteremia while receiving high-dose cytarabine chemotherapy for acute myeloid leukemia. The TM49T characterized at species level through polyphasic taxonomic analysis, incorporating morphological, physiological, biochemical, phylogenetic, genome sequence analyses. 16S rRNA gene analysis revealed that belonged genus Lachnoanaerobaculum. observed be most...
Carbonic anhydrase IX (CAIX, gene G250/MN-encoded transmembrane protein) is highly expressed in various human epithelial tumors such as renal clear cell carcinoma (RCC), but absent from the corresponding normal tissues. Besides CA signal transduction activity, CAIX may serve a biomarker early stages of oncogenesis and also reliable marker hypoxia, which associated with tumor resistance to chemotherapy radiotherapy. Although results preclinical clinical studies have shown promising target for...
An autoantibody against SS-A/Ro52 (Ro52) is most frequently found in the sera of patients with Sjögren's syndrome, systemic lupus erythematosus, and congenital heart block from anti-Ro52 Ab-positive mother. However, physiological function autoantigen has not yet been elucidated. In this study, we describe role Ro52 protein T cell activation. Overexpression Jurkat resulted enhanced IL-2 production following CD28 stimulation. Furthermore, transfection anti-Ro52-specific small RNA duplexes...
BLNK is a pivotal adaptor protein in the signal transduction pathway from IgM class B-cell receptor. phosphorylated by Syk and binds various signaling intermediates, leading to cellular events including MAP-kinase activation, culminating activation. It remains unclear how initially recruited surface (sIgM) complex which also recruited. Here we show that CMTM7, tetra-spanning membrane of unknown function, co-localized with clathrin sIgM at plasma membrane. RNA-interference-mediated knockdown...
Abstract The high binding affinities and specificities of antibodies have led to their use as drugs biosensors. Single-domain V H exhibit specificity affinity but higher stability solubility than conventional they are single-domain proteins. In this work, based on physicochemical measurements molecular dynamics (MD) simulations, we gained insight that will facilitate rational design single-chain antibodies. We first assessed two homologous by differential scanning calorimetry (DSC); one had...
Carbonic anhydrase IX (CAIX) is a tumor-associated antigen and marker of hypoxia that overexpressed on > 90% clear-cell type renal cell carcinoma (RCC) but not neighboring normal kidney tissue. Here, we report the construction two chimeric receptors (CARs) utilize carbonic (CA) domain mapped, human single chain antibody (scFv G36) as targeting moiety differ in their capacity to provide costimulatory signaling for optimal T proliferation tumor killing. The resulting anti-CAIX CARs were...
Abstract Antigen-combining sites of the camelid heavy-chain antibody variable domain (VHH) are constructed by three complementarity-determining regions (CDR1, CDR2 and CDR3). We prepared cDNA using mRNA extracted from peripheral lymphocytes alpacas that had been non-immunized or immunized with human serum albumin (HSA). The VHH gene fragments encoding amino-terminal half-containing CDR1 as well carboxy-terminal CDR3 were amplified independently PCR, then full-length generated overlap...
We searched for memory B cells responsible high-affinity anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibody production by C57BL/6 mice immunized with NP-chicken γ-globulin (CGG), using flow cytometry. first prepared transfectants expressing B-cell antigen receptor (BCR) of known affinity as a model well NP-allophycocyanin (APC) different NP valences, NP(lo), NP(med) and NP(hi). then used the latter probes capable distinguishing BCR affinities: NP(lo)-APC bound to BCRs an higher than 3.4 ×...
We evaluated the potential of an anti–human immunodeficiency virus (HIV) Tat intrabody (intracellular antibody) to promote survival CD4+ cells after chimeric simian (SIV)/HIV (SHIV) infection in rhesus macaques. Following optimization stimulation and transduction conditions, purified T were transduced with GaLV-pseudotyped retroviral vectors expressing either anti-HIV-1 or a control single-chain intrabody. Ex vivo intrabody-gene marking was highly efficient, averaging four copies per cell....
Laryngeal papilloma (LP) associated with human papillomavirus (HPV)-6 or -11 infection shows aggressive growth. However, the detailed molecular mechanism of virus-driven tumorigenesis has not been uncovered fully. HPV-6 viral gene expression and dynamic alterations were investigated in situ localization DNA RNA 13 patients HPV-6-infected laryngeal papilloma. The average load was 4.80 × 105 ± 1.86 copies/ng DNA. E4, E5a, E5b mRNAs accounted for 96% 9 mRNAs. alteration during recurrence...