A5066266244- Skin Protection and Aging
- melanin and skin pigmentation
- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Cell death mechanisms and regulation
- Antioxidant Activity and Oxidative Stress
- Circadian rhythm and melatonin
- bioluminescence and chemiluminescence research
- Plant chemical constituents analysis
- Genomics, phytochemicals, and oxidative stress
- Wnt/β-catenin signaling in development and cancer
- Melanoma and MAPK Pathways
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Synthesis of Organic Compounds
- NF-κB Signaling Pathways
- Curcumin's Biomedical Applications
- Bioactive natural compounds
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Apelin-related biomedical research
Jagiellonian University
2014-2025
George Washington University
2005-2014
Washington University in St. Louis
2005-2013
Washington University Medical Center
2005-2007
Malignant melanoma of the skin (CMM) is associated with ultraviolet radiation exposure, but mechanisms and even wavelengths responsible are unclear. Here we use a mammalian model to investigate formed in response precise spectrally defined biologically relevant doses. We show that induction by A (320–400 nm) requires presence melanin pigment oxidative DNA damage within melanocytes. In contrast, B (280–320 initiates pigment-independent manner direct damage. Thus, identified two...
The protein p53 protects the organism against carcinogenic events by induction of cell cycle arrest and DNA repair program upon damage. Virtually all cancers inactivate either mutations/deletions TP53 gene or boosting negative regulation activity. overexpression MDM2 is one most common mechanisms utilized p53wt to keep inactive. Inhibition action its antagonists has proved anticancer potential in vitro now tested clinical trials. However, prolonged treatment cells with leads development...
Vemurafenib and dabrafenib are BRAF kinase inhibitors (BRAFi) used for the treatment of patients with melanoma carrying V600E mutation. However, cells develop resistance to both drugs when as monotherapy. Therefore, mechanisms drug investigated, new molecular targets sought that could completely inhibit progression. Since receptor-interacting protein (RIPK4) probably functions an oncogene in its structure is similar protein, we analyzed impact vemurafenib on RIPK4 melanomas. The silico study...
Melatonin (N-acetyl-5-methoxytryptamine) is recognized as an effective antioxidant produced by the pineal gland, brain and peripheral organs, which also has anti-inflammatory, immunomodulatory, anti-tumour capacities. been reported a substance that counteracts ultraviolet radiation B (UVB)-induced intracellular disturbances. Nevertheless, mechanistic actions of related molecules including its kynurenic derivatives (N1-acetyl-N2-formyl-5-methoxykynurenine (AFMK)), indolic (6-hydroxymelatonin...
Abstract Mechanisms of juvenile susceptibility to cancer are not well understood. The immune response in neonates favors nonresponsiveness or TH2-dominant responses, raising the question a role for neonatal immunity this susceptibility. We have investigated postulate that inflammatory differs and adult skin. found no infiltrate into mouse skin UV irradiation as function time, dose, wavelength, although UV-induced DNA damage was readily detected. In contrast, mice initiated dose-...
Abstract Purpose The receptor-interacting protein kinase (RIPK4) has an oncogenic function in melanoma, regulates NF-κB and Wnt/β-catenin pathways, is sensitive to the BRAF inhibitors: vemurafenib dabrafenib which lead its decreased level. As role melanoma remains not fully understood, we examined effects of downregulation on transcriptomic profile melanoma. Methods Applying RNA-seq, revealed global alterations transcriptome WM266.4 cells with RIPK4 silencing. Functional partners were...
Recent studies focus on usage of blue light λ = 450 nm in combination with photosensitizers to treat surface skin disorders, including cancers. In search convenient therapeutic factor we studied riboflavin analogue 3-methyl-tetraacetylriboflavin (3MeTARF) as potential sensitizer. Riboflavin (Rfl) itself, non -toxic the darkness, upon absorption UVA and light, may act photosensitizer. However, Rfl efficiency is limited due its susceptibility photodecomposition. Riboflavin's acetylated...
Curcumin is a plant-derived yellow-orange compound widely used as spice, dye and food additive. It also believed to have therapeutic effects against different disorders. On the other hand, there are data showing its phototoxicity bacteria, fungi various mammalian cells. Since mechanism of phototoxic action not fully understood, we investigated here potential curcumin in liposomal model membranes HaCaT First, detection singlet oxygen (1 O2 ) luminescence proved that generates 1 upon blue...
Among the substances that attracted attention of oncologists in recent years are selenium-containing compounds, both inorganic and organic. Several epidemiological studies have shown an inverse correlation between selenium intake cancer incidence. In experiments reported here, we compared effects 2 selenium- containing salts differed level oxidation, selenite IV selenate VI. We tested these compounds on cell survival growth, cycle processing, morphology, cytoskeleton, lipid peroxidation 3...
Melanins are an important factor determining the vulnerability of mammalian skin to UV radiation and thus UV-induced cancers. Transgenic mice overexpressing hepatocyte growth factor/scatter (HGF/SF) have extra-follicular dermal melanocytes, notably in papillary upper dermis, susceptible melanoma. Pigmented HGF/SF neonatal more than albino animals UVA -induced melanoma, indicating involvement melanin melanoma formation. This raises question effect transgenic on melanization. We developed a...