A5106126887- Antimicrobial Resistance in Staphylococcus
- Intestinal Malrotation and Obstruction Disorders
- Congenital gastrointestinal and neural anomalies
- Pediatric Urology and Nephrology Studies
- Bacterial Genetics and Biotechnology
- Pelvic and Acetabular Injuries
- Hernia repair and management
- Tracheal and airway disorders
- Helicobacter pylori-related gastroenterology studies
- Cardiac, Anesthesia and Surgical Outcomes
- Bacterial biofilms and quorum sensing
- Esophageal and GI Pathology
- Urological Disorders and Treatments
- Mycobacterium research and diagnosis
- Congenital Diaphragmatic Hernia Studies
- Testicular diseases and treatments
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Gastrointestinal disorders and treatments
- Appendicitis Diagnosis and Management
- Antibiotic Resistance in Bacteria
- Colorectal Cancer Surgical Treatments
- Medical Image Segmentation Techniques
- Radiomics and Machine Learning in Medical Imaging
- Veterinary medicine and infectious diseases
Normandie Université
2017-2025
Université de Caen Normandie
2006-2025
Hôpital Necker-Enfants Malades
2018-2025
Assistance Publique – Hôpitaux de Paris
2013-2025
Université Paris Cité
2012-2025
Institut des Maladies Génétiques Imagine
2017-2025
Communication Bactérienne et Stratégie Anti-infectieuses
2023-2024
IMT Mines Alès
2024
Université de Montpellier
2024
EuroMov Digital Health in Motion
2024
Summary Fur, the ferric uptake regulator, is a transcription factor that controls iron metabolism in bacteria. Binding of ferrous to Fur triggers conformational change activates protein for binding specific DNA sequences named boxes. In Helicobacter pylori , HpFur involved acid response and important gastric colonization model animals. Here we present crystal structure functionally active mutant (HpFur2M; C78S‐C150S) bound zinc. Although its fold similar other Fur‐like proteins, reveals...
Lactic acid bacteria (LAB) strains OB14 and OB15 were isolated from traditional Tunisian fermented dairy products, Testouri cheese Rigouta, respectively. They identified as Enterococcus faecalis by the MALDI TOF-MS (matrix assisted laser desorption-ionization time of flight mass spectrometry) biotyper system molecular assays (species-specific PCR). These new isolates evaluated for probiotic properties, compared to E. Symbioflor 1 clone DSM 16431, reference. The found be tolerant harsh...
We present the preliminary results of robotic-assisted laparoscopic (RAL) total and partial nephrectomy for renal malignant tumors in children.This is a prospective study patients operated with RAL between December 2016 September 2018. Patients Wilms were treated according to SIOP-2001 protocol. Patient tumor characteristics, type surgery, surgical-related morbidity, oncologic outcomes recorded. Results compared series similar age- tumor-related characteristics during same period by an open...
The transition metal nickel plays a central role in the human gastric pathogen Helicobacter pylori because it is required for two enzymes indispensable colonization, metalloenzyme urease and [NiFe] hydrogenase. To sustain availability these metalloenzymes while providing protection from metal's harmful effects, H. equipped with several specific nickel-binding proteins. Among these, possesses particular chaperone, HspA, that homolog of highly conserved essential bacterial heat shock protein...
Nickel is an essential metal for Helicobacter pylori, as it the co-factor of two enzymes crucial colonization, urease and hydrogenase. taken up by specific transporters its intracellular homeostasis depends on nickel-binding proteins to avoid toxicity. trafficking controlled Ni(II)-dependent transcriptional regulator NikR. In contrast other NikR proteins, from H. pylori a pleiotropic that depending target gene acts activator or repressor. We systematically quantified in vivo Ni(2+)-NikR...
Abstract Microbial endocrinology has demonstrated for more than two decades, that eukaryotic substances (hormones, neurotransmitters, molecules of the immune system) can modulate physiological behavior bacteria. Among them, hormones/neurotransmitters, epinephrine (Epi) and norepinephrine (NE), released in case stress, physical effort or used medical treatment, were shown to be able modify biofilm formation various bacterial species. In present study, we have evaluated effect Epi on motility,...
Ovarian mature teratoma represents the most common benign neoplasm among pediatric germ cell tumors. This study reports prevalence and characteristics of familial forms identified in a single center over 22 years order to better understand possible predispositions ovarian teratoma. The records all patients who were surgically treated for between 2000 2022 retrospectively reviewed. Families systematically asked about family history tumors (benign or malignant) testicular Among 136 operated on...
Two PTS transporters involved in the uptake of cellobiose and short cellooligosaccharides were identified Enterococcus faecalis. Genes coding for different EII proteins are found a locus composed three operonic structures expressing two distinct EIIC (CelC1 CelC2), identical EIIB (CelB1 CelB2) unique EIIA (CelA1). The plays central role β-glucoside because it is required not only β-homodiholosides but also diheteroside N-acetylglucosamine-L-asparagine. Depending on their size, preferably...
Anorectal malformations (ARMs) are congenital anomalies of the distal part hindgut often associated with sacral and/or spinal anomalies. We investigated anatomical and microstructural properties lumbosacral plexus ARM patients from imaging data. Twenty-five (16 males), median age 4 months (2-49), 13 high 12 low ARM, underwent 3 Tesla magnetic resonance diffusion tensor sequences (dMRI) before repair. A 3D model was built manual segmentation used to guide novel AI algorithms for nervous...
ABSTRACT Analysis of the genome sequence Enterococcus faecalis allowed identification two genes whose protein products showed 33 and 34% identity with those sigV yrhM Bacillus subtilis , respectively. These genes, named rsiV are predicted to encode members extracytoplasmic function subfamily eubacterial RNA polymerase sigma anti-sigma factors, This group factors has been shown regulate gene expression in response stress conditions. were be under control same promoter. The transcriptional...
Enterococcus faecalis is a Gram-positive lactic acid intestinal opportunistic bacterium with virulence potential. For better understanding of the adapation this to host conditions, we performed transcriptome analysis bacteria isolated from an infection site (mouse peritonitis) by RNA-sequencing. We identified total 211 genes significantly higher transcript levels and 157 repressed genes. Our in vivo gene expression database reflects well process since encoding important factors like...
Simian immunodeficiency viruses have been isolated from African green monkeys originating Ethiopia. A molecular clone, termed SIVagm3, was found to be highly divergent SIVagmTYO-1 in terms of its restriction map and partial nucleotide sequence. premature stop codon present the transmembrane protein SIVagm TYO-1 absent SIVagm3. SIVagm3 biologically active vitro vivo displayed characteristics reminiscent wild-type virus. Biological activity demonstrated by seroconversion juvenile Macaca...
The alternative sigma factor sigma(E) is activated by unfolded outer membrane proteins (OMPs) and plays an essential role in Salmonella pathogenesis. canonical pathway of activation response to envelope stress involves sequential proteolysis the anti-sigma RseA PDZ proteases DegS RseP. Here we show that enterica sv. Typhimurium can also be acid stress. A sigma(E)-deficient mutant exhibits increased susceptibility pH reduced survival acidified phagosomal vacuole. Acid sigma(E)-dependent gene...
NikR is a transcriptional metalloregulator central in the mandatory response to acidity of Helicobacter pylori that controls expression numerous genes by binding specific promoter regions. NikR/DNA interactions were proposed rely on protein activation Ni(II) high-affinity (HA) and possibly secondary external (X) sites. We describe biochemical characterization HpNikR mutants shows HA sites are essential but not sufficient for DNA binding, while residues from dimer–dimer interface important...
Enterococcus faecium and faecalis are Gram-positive opportunistic pathogens that have become leading causes of nosocomial infections over the last decades. Especially multidrug resistant enterococci a challenging clinical problem worldwide. Therefore, new treatment options needed identification alternative targets for vaccine development has emerged as feasible to fight caused by these pathogens.We extrapolate transcriptomic data from mice peritonitis infection model in E. identify putative...
The Enterococcus faecalis two-component signal transduction system CroRS, also referred as the RR-HK05 pair, is required for intrinsic beta-lactam resistance (Y. R. Comenge, Quintiliani, Jr., L. Li, Dubost, J. P. Brouard, E. Hugonnet, and M. Arthur, Bacteriol. 185:7184-7192, 2003) suspected to be involved in expression of salB (previously sagA), a gene important environmental stress cell morphology Le Breton, G. Boël, A. Benachour, H. Prévost, Y. Auffray, Rincé, Environ. Microbiol....
Using a modified random arbitrarily primed PCR approach, the operon encoding Enterococcus faecalis JH2-2 CroRS two-component regulatory system was shown to be repressed during stationary phase, and CroRS-regulated (glnQHMP) identified. Gel retardation assays showed that CroR regulator binds specifically glnQHMP promoter.