A5029077510- Forensic Toxicology and Drug Analysis
- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Stress Responses and Cortisol
- Nuclear Receptors and Signaling
- Metabolomics and Mass Spectrometry Studies
- Alcohol Consumption and Health Effects
- Anesthesia and Neurotoxicity Research
- Neurological and metabolic disorders
- Cardiovascular Conditions and Treatments
- Neuropeptides and Animal Physiology
- Congenital Heart Disease Studies
- Advanced Chemical Sensor Technologies
- Child and Adolescent Psychosocial and Emotional Development
- Hormonal and reproductive studies
- Metabolism and Genetic Disorders
- Veterinary Pharmacology and Anesthesia
- Receptor Mechanisms and Signaling
- Mechanical Circulatory Support Devices
- Peroxisome Proliferator-Activated Receptors
- Identification and Quantification in Food
- Molecular Biology Techniques and Applications
- Cancer, Stress, Anesthesia, and Immune Response
- Cell Image Analysis Techniques
- Mass Spectrometry Techniques and Applications
Universidade do Porto
2012-2025
Rede de Química e Tecnologia
2012-2013
Zero to Three
2013
Instituto Português de Oncologia Francisco Gentil
2013
Cooperativa de Ensino Superior Politécnico e Universitário
2013
IPO Porto
2012
ABSTRACT Over recent years, hair has become the ideal matrix for retrospective investigation of chronic abuse, including tramadol. However, in order to exclude possibility external contamination, it is also important quantify simultaneously its main metabolite, O ‐desmethyltramadol (M1), which presence reflects systemic exposure. In present study a methodology aimed at simultaneous quantification tramadol and M1 human was developed validated first time. After decontamination samples (60 mg),...
ABSTRACT The development of analytical techniques that enable the use hair as an alternative matrix for analysis drugs abuse is useful confirming exposure in a larger time window (weeks to months, depending on length shaft). In present study methodology aimed at simultaneous quantification cocaine and morphine human was developed validated. After decontamination, samples (20 mg) were incubated with mixture methanol/hydrochloric acid (2:1) 65 °C overnight (~16 h) order extract matrix....
The importance of catecholamines in post-traumatic stress disorder (PTSD) still needs to be explored. We aimed evaluate epinephrine's (EPI) causal role and molecular mechanism for the persistence PTSD traumatic memories. Wild-type (WT) EPI-deficient mice (phenylethanolamine-N-methyltransferase-knockout mice, Pnmt-KO) were induced with behavioral tests performed. Some Pnmt-KO administered EPI or vehicle. Catecholamines quantified by HPLC-ED. Nr4a1, Nr4a2, Nr4a3 mRNA expression evaluated...
Epinephrine (EPI) strengthens contextual fear memories by acting on peripheral β2-adrenoceptors. Phenylethanolamine-N-methyltransferase-knockout mice (Pnmt-KO) are EPI-deficient and have reduced learning. Our aim was to evaluate the molecular mechanisms which EPI memory if a β2-adrenoceptor antagonist can erase memories. Pnmt-KO wild-type (WT) were submitted conditioning procedure after treatment with EPI, norepinephrine (NE), plus ICI 118,551 (selective antagonist), or vehicle (NaCl 0.9%)....
The processes by which fear memory is encoded, consolidated, and re-consolidated are extremely complex appear to require the release of stress hormones, especially adrenaline (AD). AD improves contextual memory, acting specifically on peripheral β2-adrenoceptors. Propranolol (peripheral central β-adrenoceptor antagonist) treatment was shown prevent post-traumatic disorder (PTSD) development reduce its symptoms. However, propranolol has several side effects. Thus, we aimed evaluate if sotalol...
Adrenaline enhances contextual fear memory consolidation possibly by activating liver β2-adrenoceptors causing transient hyperglycaemia. Contrastingly, insulin-induced hypoglycaemia may culminate in blood adrenaline increment, hidering the separation of each hormone's action memory. Therefore, an Ad-deficient mouse model was used aiming to investigate if following insulin administration requires or not subsequent increases plasma adrenaline, which occurs response hypoglycemia. Fear...
Post-traumatic stress disorder (PTSD) is a common anxiety mental and can be manifested after exposure to real or perceived life-threatening event. Increased noradrenaline adrenaline in plasma urine have been documented PTSD. Dopamine-β-hydroxylase (DBH) catalyzes the conversion of dopamine consequently, DBH inhibition reduces catecholamines. Our aim was evaluate if nepicastat treatment decreases PTSD signs an animal model. Wild-type (129x1/SvJ) female mice were submitted induction protocol....
Abstract Adrenaline (Ad) and glucose released into the bloodstream during stress may strengthen contextual fear memory. However, no previous studies have detached effects of from Ad in this paradigm. Using Ad-deficient mice, we aimed to evaluate effect on memory when endogenous is absent. Fear conditioning was performed wild-type (WT) mice (129 × 1/SvJ) administered with (30 or 10 mg/kg; i.p.) or/and (0.01 vehicle (0.9% NaCl; i.p.). Catecholamines were quantified using HPLC-ED. Real-time...
Introduction: Hemodynamically significant patent ductus arteriosus (PDA) is a common condition in very low birth weight infants. Therapeutic options include medical therapy and surgical ligation. Medical treatment based on non-selective inhibitors of cyclooxygenases 1 2 (indomethacin ibuprofen). The debate the most appropriate for closure PDA far from being closed, light currently available evidence. Aim: objective this study was to compare efficacy safety indomethacin ibuprofen. Methods:...