A5100603793- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Turfgrass Adaptation and Management
- RNA modifications and cancer
- Aquaculture disease management and microbiota
- Aquatic Ecosystems and Phytoplankton Dynamics
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Radioactivity and Radon Measurements
- Hemoglobin structure and function
- Glutathione Transferases and Polymorphisms
- Radiation Detection and Scintillator Technologies
- Invertebrate Immune Response Mechanisms
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Enzyme Structure and Function
- Marine and coastal ecosystems
- Environmental Toxicology and Ecotoxicology
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- RNA Research and Splicing
- Virus-based gene therapy research
- Metal-Organic Frameworks: Synthesis and Applications
- Biocrusts and Microbial Ecology
- Synthesis and Characterization of Heterocyclic Compounds
Hengyang Normal University
2023-2025
East China Normal University
2004-2025
Southern Medical University
2022-2025
China Medical University
2024-2025
Southern University of Science and Technology
2025
Southwest University
2025
Chinese PLA General Hospital
2025
Sichuan University
2010-2025
West China Hospital of Sichuan University
2010-2025
Northwest A&F University
2025
It is widely understood that CRISPR-Cas9 technology revolutionary, with well-recognized issues including the potential for off-target edits and attendant need spatiotemporal control of editing. Here, we describe a far-red light (FRL)-activated split-Cas9 (FAST) system can robustly induce gene editing in both mammalian cells mice. Through light-emitting diode-based FRL illumination, FAST efficiently edit genes, nonhomologous end joining homology-directed repair, multiple loci human cells....
The mitogen-activated protein kinase (MAPK) cascade is an ancient and evolutionarily conserved signaling pathway involved in numerous physiological processes. Despite great advances understanding MAPK-mediated regulation of adaptive immune responses mammals, its contribution to T-cell immunity early vertebrates remains unclear. Herein, we used Nile tilapia (Oreochromis niloticus) investigate the regulatory roles MAPK/extracellular signal-regulated (Erk) ancestral jawed fish. We found that...
Exposure to environmentally hazardous substances is recognized as a significant risk factor for neurological associated disorders. Among these substances, polystyrene microplastics (PS-MPs), widely utilized in various consumer products, have been reported exhibit neurotoxicity. However, the potential association of PS-MPs with abnormal anxiety behaviors, along underlying molecular mechanisms and key proteins involved, remains insufficiently explored. Here, we delineated PS-MPs-induced...
Adipocyte fatty acid-binding protein (A-FABP) is up-regulated in regenerated endothelial cells and modulates inflammatory responses macrophages. Endothelial dysfunction accompanying regeneration accelerated by hyperlipidaemia. Here, we investigate the contribution of A-FABP to pathogenesis aorta apolipoprotein E-deficient (ApoE(-/-) ) mice cultured human cells.A-FABP was measured aortae ApoE(-/-) RT-PCR, immunostaining immunoblotting. Total phosphorylated forms nitric oxide synthase (eNOS)...
Both genetic and microenvironmental detrimental factors are involved in ovarian dysfunction, leading to the increasing rate of involuntary childlessness recent years. Oxidative stress (OS), which is characterized by imbalance redox system with redundant reactive oxygen species (ROS) overwhelming antioxidant defense, regarded as one culprits dysfunction. OS causes damage various types cells including granulosa (GCs), jeopardizing microenvironment, disturbing follicular development...
The Gram-negative bacterium Pseudomonas aeruginosa contains a heme oxygenase (pa-HO) that primarily oxygenates the δ-meso carbon [Caignan, G. A., Deshmukh, R., Wilks, Zeng, Y., Huang, H. W., Moenne-Loccoz, P., Bunce, R. Eastman, M. and Rivera, (2002) J. Am. Chem. Soc. 124, 14879−14892]. This differs from other previously characterized oxygenases, which display regioselectivity for α-meso carbon. Here we report crystal structure of pa-HO at 1.60 Å resolution compare it to 1.50 nm-HO Neisseria...
The ferryl [Fe(IV)O] intermediate is important in many heme enzymes, and thus, the precise nature of Fe(IV)−O bond critical understanding enzymatic mechanisms. 1.40 Å crystal structure cytochrome c peroxidase Compound I has been determined as a function X-ray dose while visible spectrum was being monitored. Fe−O increases length from 1.73 low-X-ray to 1.90 high-dose structure. low-dose correlates well with an Fe(IV)═O bond, we postulate that cryo-trapped Fe(III)−OH species previously thought...
Lead (Pb) is one of the most toxic heavy metal environmental pollutants. Tall fescue an important cold season turf grass which can tolerate and accumulate substantial amount Pb. To estimate genes related to Pb response molecular mechanism associated with tolerance accumulation, we analyzed transcriptome tall in treatment.RNA-sequencing was performed two cultivars, tolerant Silverado sensitive AST7001. A total 810,146 assembled unique transcripts representing 25,415 unigenes were obtained...
Human noroviruses are the most important viral pathogens causing epidemic acute gastroenteritis, in which GII.4 viruses have been predominant worldwide for past decades. During 2014–2015 winter season, a new GII.17 variant emerged as virus China surpassing significantly increased gastroenteritis outbreaks. Genome sequences of was determined and compared with other noroviruses, revealing residue substitutions at specific locations, including histo-blood group antigen-binding site putative...
T cells suddenly appeared in jawed fish ∼450 million years ago. Biological studies of may provide helpful evidence to understand evolution adaptive immune systems. To this end, using a Nile tilapia (Oreochromis niloticus) model, we revealed the regulatory mechanism immunity mediated by ancestral fish. as well tightly regulated mammalian/mechanistic target rapamycin complex 1 (mTORC1) pathway participate cellular response during Streptococcus agalactiae infection. Blockade mTORC1 signaling...
Background: N6-methyladenosine (m 6 A) RNA methylation is dynamically and reversibly regulated by methyl-transferases ("writers"), binding proteins ("readers"), demethylases ("erasers").The m A restored to adenosine thus achieve demethylation modification.The abnormality of epigenetic modification in cancer has been increasingly attended.However, we are rarely aware its diagnostic, progressive prognostic performance lung adenocarcinoma (LUAD).Methods Results: The expression 13 widely...
The toxicity and related mechanisms of aflatoxin B1 (AFB1) M1 (AFM1) in the mouse kidney were studied, role l-proline alleviating damage was investigated. In a 28-day model, thirty mice divided into six groups: control (without treatment), group (10 g/kg body weight (b.w.)), AFB1 (0.5 mg/kg b.w.), AFM1 (3.5 + group. Kidney index biochemical indicators detected, pathological staining observed. Using human embryonic 293 (HEK 293) cell apoptosis rate apoptotic proteins expressions detected....
An essential component of heme transport in Gram-negative bacterial pathogens is the periplasmic protein that shuttles between outer and inner membranes. We have solved first crystal structures two such proteins, ShuT from Shigella dysenteriae PhuT Pseudomonas aeruginosa. Both share a common architecture typical Class III binding proteins. The binds narrow cleft N- C-terminal domains coordinated by Tyr residue. A comparison heme-free (apo) -bound (holo) indicates little change structure...